EKS-DRM-00015

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DRM-00015

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CK1/VRK	358.0	3.4E-107	47	310	264

Status

Reviewed

Ensembl Protein

FBpp0084465

UniProt Accession

Q7KRY6; Q9U9Q0; Q9VBB2;

Protein Name

Nucleosomal histone kinase 1

Protein Synonyms/Alias

Protein baellchen;

Gene Name

ball

Gene Synonyms/Alias

ball; ballchen, nhk-1; CG6386;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
FBgn0027889	FBpp0084465	FBtr0085095
FBgn0027889	FBpp0084466	FBtr0085096

Organism

Drosophila melanogaster

Functional Description

Serine/threonine kinase involved in somatic mitosis andfemale meiosis. Required for spindle organization in mitosis, and for the establishment or maintenance of meiosis-specific chromosomal configurations, including the prophase I karyosome and the metaphase I spindle. Specifically phosphorylates nucleosomal H2A on 'Thr-119'.

Protein Length

599

Protein Sequence
(FASTA)

MPRVAKPKAA APAKKVVSAK KAKSKLYKMP EKVKEGTVFT DLAKGQWRIG PSIGVGGFGE 60

IYAACKVGEK NYDAVVKCEP HGNGPLFVEM HFYLRNAKLE DIKQFMQKHG LKSLGMPYIL 120

ANGSVEVNGE KHRFIVMPRY GSDLTKFLEQ NGKRLPEGTV YRLAIQMLDV YQYMHSNGYV 180

HADLKAANIL LGLEKGGAAQ AYLVDFGLAS HFVTGDFKPD PKKMHNGTIE YTSRDAHLGV 240

PTRRADLEIL GYNLIEWLGA ELPWVTQKLL AVPPKVQKAK EAFMDNIGES LKTLFPKGVP 300

PPIGDFMKYV SKLTHNQEPD YDKCRSWFSS ALKQLKIPNN GDLDFKMKPQ TSSNNNLSPP 360

GTSKAATARK AKKIDSPVLN SSLDEKISAS EDDEEEEEKS HRKKTAKKVT PSARNAKVSP 420

LKRVADSSPP SQKRVKTEPK STPRERATPK ASPKPRSTPK ASPKPQTPTA ARLRTPNAKI 480

NFSPSISLRG RPGGKTVIND DLTPQPRSKK TYEFNFELDV SMDANVIVNV KRKKKADQDK 540

ATAVDSRTPS SRSALASSSK EEASPVTRVN LRKVNGHGDS STPGRSPRTP AVTVRKYQG 599

Nucleotide Sequence
(FASTA)

ATGCCGCGTG TAGCCAAGCC GAAAGCCGCC GCTCCGGCCA AGAAGGTGGT GTCGGCCAAG 60

AAGGCCAAGA GCAAGCTGTA CAAGATGCCG GAGAAGGTGA AGGAGGGCAC AGTTTTCACC 120

GATTTAGCCA AAGGCCAGTG GCGCATTGGA CCCTCGATCG GAGTTGGAGG ATTCGGTGAG 180

ATCTACGCGG CTTGCAAAGT GGGTGAGAAG AACTACGATG CTGTGGTCAA ATGCGAGCCA 240

CATGGCAATG GTCCTCTGTT CGTGGAGATG CACTTCTACC TGCGCAACGC CAAGCTGGAG 300

GACATCAAAC AGTTCATGCA GAAGCACGGT CTCAAGTCCC TGGGCATGCC CTACATACTG 360

GCTAATGGTT CCGTGGAGGT CAATGGTGAA AAGCATCGAT TTATAGTCAT GCCGCGCTAC 420

GGCAGCGATT TGACCAAGTT TCTGGAACAG AACGGCAAGC GACTGCCCGA GGGCACTGTC 480

TACCGACTGG CCATTCAGAT GCTGGACGTC TATCAGTACA TGCACAGCAA CGGCTACGTT 540

CATGCCGATC TTAAGGCAGC CAACATACTG CTGGGCCTGG AAAAAGGCGG CGCTGCGCAG 600

GCGTACCTGG TGGATTTCGG CCTGGCCTCA CACTTCGTCA CCGGTGACTT CAAGCCGGAC 660

CCCAAGAAGA TGCATAATGG CACCATAGAA TACACATCAA GGGATGCTCA TCTGGGCGTG 720

CCAACCAGAC GAGCTGATCT GGAGATACTC GGTTACAATC TCATCGAGTG GCTGGGCGCG 780

GAGTTGCCGT GGGTCACGCA GAAACTACTG GCTGTACCCC CCAAAGTTCA AAAGGCCAAG 840

GAAGCATTTA TGGATAACAT AGGCGAGAGT CTGAAGACGC TGTTTCCGAA GGGAGTGCCC 900

CCGCCAATTG GGGATTTCAT GAAGTATGTC TCAAAACTAA CACACAACCA GGAGCCGGAC 960

TACGACAAGT GTCGCAGTTG GTTCTCAAGT GCGCTGAAGC AGCTGAAAAT TCCAAACAAC 1020

GGAGATCTCG ACTTTAAGAT GAAGCCACAG ACCAGCAGCA ACAATAATCT AAGTCCGCCC 1080

GGCACGAGCA AGGCAGCAAC AGCCAGGAAA GCAAAAAAAA TTGATTCGCC AGTCTTGAAC 1140

TCATCACTGG ACGAAAAAAT CTCCGCCAGC GAAGATGATG AGGAGGAGGA GGAGAAGTCA 1200

CATCGCAAGA AGACAGCCAA AAAGGTCACC CCATCAGCCC GAAACGCCAA GGTATCGCCC 1260

TTGAAGCGAG TCGCAGATAG TTCACCACCC AGCCAGAAGC GCGTTAAGAC TGAGCCCAAG 1320

TCAACACCCA GGGAGAGAGC TACGCCCAAG GCCAGTCCCA AGCCAAGGAG CACGCCGAAG 1380

GCCAGCCCCA AGCCACAGAC ACCAACAGCA GCGCGGCTCC GTACTCCCAA TGCCAAGATC 1440

AATTTCAGCC CGTCTATTTC GCTGCGTGGA CGTCCCGGTG GCAAAACCGT GATCAACGAT 1500

GACCTCACTC CACAGCCGCG TTCCAAGAAG ACCTATGAGT TTAACTTCGA GCTCGACGTT 1560

AGCATGGATG CCAACGTAAT TGTGAATGTC AAACGCAAAA AGAAAGCGGA CCAGGATAAG 1620

GCGACGGCGG TTGATTCACG TACACCCTCA TCCCGCAGCG CGTTGGCGTC CAGTTCGAAG 1680

GAGGAGGCCT CACCTGTGAC CCGAGTTAAC CTGCGCAAGG TAAACGGCCA TGGCGACTCC 1740

TCCACGCCCG GCCGTAGTCC GCGGACACCA GCCGTCACTG TGCGGAAATA CCAGGGATAG 1800

Domain Profile

S: 1     wklgkkigkggfGeiylaltckkeekdaklvvkvepkenGpLfvelkfyqraakkeqikk  60

         w++g +ig ggfGeiy+a  ck +ek++++vvk+ep+ nGpLfve++fy r+ak e+ik+

Q: 47    WRIGPSIGVGGFGEIYAA--CKVGEKNYDAVVKCEPHGNGPLFVEMHFYLRNAKLEDIKQ  104

         9***************88..****************************************

S: 61    wkkkkklkllgiPtliasGlhefegekyRflvlprlgrdLqklleqngkrlsektvlqla  120

         +++k+ lk+lg+P+++a+G++e++gek+Rf+v+pr+g+dL+k+leqngkrl e tv++la

Q: 105   FMQKHGLKSLGMPYILANGSVEVNGEKHRFIVMPRYGSDLTKFLEQNGKRLPEGTVYRLA  164

         ************************************************************

S: 121   vrlldvleylHeneYvHgdikAanllleeee..asqvyLvdyGlayrycpegkhkeyked  178

         +++ldv++y+H+n+YvH+d+kAan+ll+ e+  a+q+yLvd+Gla++++++    ++k+d

Q: 165   IQMLDVYQYMHSNGYVHADLKAANILLGLEKggAAQAYLVDFGLASHFVTG----DFKPD  220

         ***************************9998889***************99....8****

S: 179   krraHdGtleftslDaHkGvapsrRsDleiLgYcllkWltgkLPW..eallkdpekvqka  236

         ++++H+Gt+e+ts+DaH Gv p+rR+DleiLgY+l++Wl ++LPW  ++ll+ p kvqka

Q: 221   PKKMHNGTIEYTSRDAHLGV-PTRRADLEILGYNLIEWLGAELPWvtQKLLAVPPKVQKA  279

         ********************.************************988899*********

S: 237   kekfldelpellkkllkkksss..eelakyl  265

         ke+f+d+++e+lk+l++k++++  ++++ky+

Q: 280   KEAFMDNIGESLKTLFPKGVPPpiGDFMKYV  310

         *******************998667888885

Domain Sequence
(FASTA)

WRIGPSIGVG GFGEIYAACK VGEKNYDAVV KCEPHGNGPL FVEMHFYLRN AKLEDIKQFM 60

QKHGLKSLGM PYILANGSVE VNGEKHRFIV MPRYGSDLTK FLEQNGKRLP EGTVYRLAIQ 120

MLDVYQYMHS NGYVHADLKA ANILLGLEKG GAAQAYLVDF GLASHFVTGD FKPDPKKMHN 180

GTIEYTSRDA HLGVPTRRAD LEILGYNLIE WLGAELPWVT QKLLAVPPKV QKAKEAFMDN 240

IGESLKTLFP KGVPPPIGDF MKYV 264

Keyword

ATP-binding; Cell cycle; Cell division; Chromatin regulator; Chromosome; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Meiosis; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0000785	; C:chromatin
GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0035184	; F:histone threonine kinase activity
GO:0051301	; P:cell division
GO:0007143	; P:female meiosis
GO:0030717	; P:karyosome formation
GO:0000070	; P:mitotic sister chromatid segregation
GO:0007052	; P:mitotic spindle organization
GO:0022008	; P:neurogenesis
GO:0007130	; P:synaptonemal complex assembly

KEGG

dme:Dmel_CG6386

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013