EKS-DRM-00026

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DRM-00026

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	371.6	3.1E-111	804	1098	295

Status

Reviewed

Ensembl Protein

FBpp0078013

UniProt Accession

Q9VP22; Q8T9E1; Q95SE1;

Protein Name

Cyclin-dependent kinase 12

Protein Synonyms/Alias

Cell division protein kinase 12; dCdk12;

Gene Name

Cdk12

Gene Synonyms/Alias

Cdk12; CG7597;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
FBgn0037093	FBpp0078014	FBtr0078358
FBgn0037093	FBpp0078013	FBtr0078357
FBgn0037093	FBpp0304962	FBtr0332716

Organism

Drosophila melanogaster

Functional Description

Cyclin-dependent kinase which displays CTD kinaseactivity: hyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit, thereby acting as a key regulator of transcription elongation.

Protein Length

1157

Protein Sequence
(FASTA)

MHASSAAATA LVEYSDVSSE DFSDQEAGDL DADAGKGAGN IKKPKPAPDN QFSKGRLDAK 60

PDKEGYDNYR SRRAEDSSDP VAAGSRQTSS SEATNPREEP SQASNTSKDE LWGREIYMSS 120

DSIDTDELEA EMKRQKRKKQ KKEKHKHKSK KKSKKRKKKR AKSYSSIDSM SDNDINALLD 180

RRYTPPTAPS KSNERTVSAA PSSFTPHNLK ESSSPATPPP VRRPNTNSNY YGESSLETAN 240

SALGSNLQVT VTNKQSISNR LRSPPPSSRS SGNGPRFGNS PRTPPPSHYS SSGGGGVGSG 300

SVVRDSRSSR YVNSPHKEDV SAHHRSSHDH GYQGRYSGAG SSSHDTRKVK RLSPELDRYN 360

HQPSTPPHKR RKFSDGREVG LGNFEHSRHH SGKYERYSRD RYSRRSSRSP SVQHSRSRQS 420

PSGGLSSGSN AFRHGGSHKH KYGTTVSSTP SHTTRTSKRA SGTGTSGDRY SRSPRTSSRY 480

MESSPPSPVG ASGSHHYHHR RSPRMRQRTR GDSRRRSPSS ASSESSASRS RSPTSRDLKH 540

KREEYIKKIS ETSLFAELVK DRHKRQKALK EIIERQEENS NSNSNGALTI NDNSSSVDGN 600

TPNAADGRSA PGSGTPAAAS TTSNGLQALG SKPDLDLNNI PMPNKQNDSV VSNPASNADV 660

PDSVAQLKQP LLVPPFSASK NNIKPKSLTS LPLPPGMNVL DLAGARSPSP GQKKESDEKN 720

VTSSGSANKS VLNLPMPPVI PGSEELSGDD DVIDSPEDFD APAVGTVHGH GGGPGTTRQR 780

PVILNRRDSR NNVRDWGERC VDVFEMIAQI GEGTYGQVYK ARDHHTNDMV ALKKVRLEHE 840

KEGFPITAVR EIKILRQLNH RNIVNLHEIV TDKQDAVEFR KDKGSFYLVF EYMDHDLMGL 900

LESGMVDFNE ENNASIMKQL LDGLNYCHKK NFLHRDIKCS NILMNNRGKV KLADFGLARL 960

YNADDRERPY TNKVITLWYR PPELLLGEER YGPSIDVWSC GCILGELFVK RPLFQANAEM 1020

AQLETISKIC GSPVPAVWPN VIKLPLFHTL KQKKTHRRRL REDFEFMPAP ALDLLDKMLD 1080

LDPDKRITAE DALRSPWLRK INPDEMPTPQ LPTWQDCHEL WSKKRRRQMR EQQESLPPTV 1140

IASTKYQQHG ATMVGDA 1157

Nucleotide Sequence
(FASTA)

ATGCATGCAT CCTCCGCGGC GGCCACTGCA CTTGTGGAGT ACTCGGACGT CAGCTCAGAG 60

GACTTCTCGG ATCAGGAGGC AGGGGACTTG GATGCGGACG CCGGCAAGGG TGCCGGAAAC 120

ATCAAAAAAC CGAAGCCAGC TCCGGATAAT CAGTTCTCCA AAGGTCGTCT TGATGCCAAG 180

CCCGATAAAG AGGGTTATGA TAACTACAGA AGTAGAAGGG CGGAAGACTC AAGCGACCCA 240

GTGGCGGCCG GATCGAGGCA AACGTCTAGC AGCGAGGCCA CGAATCCGCG GGAGGAACCT 300

TCGCAGGCAT CCAACACCTC GAAGGACGAG CTGTGGGGCA GAGAGATATA CATGTCCAGC 360

GATTCTATCG ACACCGATGA GCTGGAGGCG GAAATGAAGC GGCAGAAGCG CAAAAAGCAG 420

AAAAAGGAAA AACACAAACA CAAGTCGAAG AAAAAGTCCA AGAAGCGCAA GAAGAAGCGG 480

GCCAAGTCAT ACTCCAGCAT CGATTCGATG TCAGACAATG ACATCAATGC CTTACTGGAC 540

CGGCGCTATA CTCCGCCGAC GGCTCCTAGC AAGAGCAACG AACGGACGGT TAGCGCTGCG 600

CCGTCCTCCT TCACCCCACA CAACCTCAAG GAGAGCAGTT CGCCAGCCAC TCCACCGCCA 660

GTGCGTCGCC CGAATACGAA CAGCAACTAC TACGGCGAGT CGTCCCTGGA GACGGCCAAC 720

TCTGCACTGG GAAGCAACCT GCAGGTCACA GTTACGAATA AGCAGTCGAT CAGCAACCGG 780

CTTCGTTCTC CTCCTCCGTC CTCCCGCTCC AGCGGCAACG GGCCGAGGTT TGGAAACTCC 840

CCGCGAACTC CACCGCCCTC TCACTACAGC TCCAGTGGCG GAGGAGGGGT TGGCAGTGGG 900

TCAGTGGTCC GCGATTCACG CAGCTCCCGC TATGTAAATA GTCCCCACAA AGAGGATGTA 960

AGTGCGCATC ACCGCTCTAG CCACGATCAC GGATATCAGG GACGTTACTC TGGTGCTGGG 1020

TCGAGCAGTC ACGATACCAG GAAGGTGAAA CGACTGTCGC CGGAGCTGGA CCGCTACAAC 1080

CATCAGCCAA GCACGCCACC CCACAAGCGT CGGAAGTTTA GCGATGGCAG GGAAGTGGGC 1140

CTCGGAAACT TTGAGCACAG TAGACACCAC TCTGGAAAGT ACGAACGATA CAGCAGAGAT 1200

CGATATTCTA GGCGGTCATC AAGGAGCCCC AGTGTGCAGC ACTCACGAAG TCGGCAATCT 1260

CCCAGCGGCG GCCTGTCAAG TGGTTCGAAC GCGTTTCGCC ATGGTGGCAG TCACAAACAC 1320

AAGTATGGGA CCACTGTTAG CTCGACGCCG TCGCATACGA CGAGGACCTC AAAACGCGCT 1380

TCGGGAACTG GTACCTCTGG CGATCGCTAC TCGAGGTCGC CCAGAACAAG TTCGCGGTAC 1440

ATGGAGTCAT CACCGCCATC GCCAGTCGGA GCTTCCGGTT CGCATCACTA TCATCATCGC 1500

CGTTCGCCAA GGATGCGCCA GAGGACCAGA GGCGACAGTC GTCGCCGGTC GCCCAGTTCG 1560

GCCAGCAGCG AAAGTTCTGC CTCTCGCAGC CGATCACCAA CGTCAAGAGA TCTAAAGCAC 1620

AAGCGCGAAG AGTACATTAA GAAGATCAGT GAGACTAGCC TATTTGCCGA GCTGGTAAAG 1680

GATCGTCACA AAAGGCAGAA GGCGCTGAAG GAAATCATTG AGCGGCAAGA GGAGAACAGC 1740

AATAGCAACA GCAACGGTGC ACTGACTATT AACGACAACA GTTCCTCCGT GGATGGCAAT 1800

ACACCCAACG CGGCTGATGG CCGATCTGCC CCTGGAAGTG GAACACCAGC AGCGGCGTCA 1860

ACAACTTCCA ATGGGCTTCA AGCTTTGGGT AGCAAGCCGG ATCTGGACCT CAACAACATT 1920

CCCATGCCAA ACAAGCAGAA CGATTCTGTG GTTTCGAATC CGGCTTCAAA CGCCGATGTT 1980

CCGGACAGTG TCGCGCAGTT GAAGCAACCT TTGCTGGTGC CACCGTTCTC GGCTAGTAAG 2040

AACAACATAA AACCGAAGAG CCTGACTTCT TTGCCGCTGC CGCCGGGTAT GAATGTGCTC 2100

GACTTGGCCG GTGCAAGGTC TCCCTCTCCG GGGCAAAAGA AGGAATCGGA CGAAAAGAAC 2160

GTTACTTCAA GCGGCAGCGC CAACAAGAGC GTGCTCAATC TTCCTATGCC GCCAGTAATT 2220

CCCGGCAGCG AAGAGCTTAG TGGCGATGAT GATGTTATCG ACAGTCCTGA AGACTTTGAT 2280

GCACCCGCGG TTGGCACTGT GCACGGTCAC GGTGGTGGGC CAGGGACAAC TCGTCAGCGC 2340

CCAGTGATAC TCAACCGTCG TGACTCGCGA AACAACGTAC GCGACTGGGG CGAGCGCTGT 2400

GTGGATGTTT TTGAGATGAT TGCGCAAATA GGGGAAGGAA CTTACGGACA GGTTTACAAG 2460

GCGCGGGATC ATCATACCAA TGACATGGTA GCACTGAAGA AGGTTCGGCT GGAGCACGAG 2520

AAGGAGGGCT TTCCCATTAC AGCTGTTCGT GAAATCAAAA TTCTGAGACA ACTTAACCAT 2580

CGCAATATAG TCAACCTGCA TGAAATTGTG ACGGACAAGC AGGATGCTGT GGAGTTCCGT 2640

AAAGACAAGG GATCCTTCTA CTTGGTGTTT GAGTACATGG ATCACGACTT GATGGGTCTG 2700

CTGGAGTCTG GCATGGTCGA CTTCAACGAG GAAAACAACG CAAGCATTAT GAAGCAGCTC 2760

TTGGACGGCC TCAACTACTG CCACAAAAAG AATTTTCTGC ATCGAGACAT CAAGTGCTCG 2820

AACATTTTAA TGAACAACAG GGGTAAAGTA AAACTAGCTG ACTTTGGGCT GGCACGACTA 2880

TACAATGCGG ACGATCGGGA ACGCCCCTAC ACGAACAAAG TAATTACCTT GTGGTATCGT 2940

CCTCCTGAGC TTTTGCTGGG TGAGGAGCGT TACGGCCCGT CGATAGACGT GTGGTCCTGC 3000

GGGTGCATCC TCGGCGAACT CTTTGTGAAG CGGCCCCTGT TCCAGGCTAA TGCGGAAATG 3060

GCGCAGCTTG AAACGATATC AAAGATCTGT GGATCCCCGG TTCCTGCCGT GTGGCCGAAT 3120

GTAATTAAGC TGCCTCTGTT CCACACTCTC AAACAAAAGA AGACGCACCG CCGCCGTCTA 3180

CGAGAAGACT TTGAATTCAT GCCAGCCCCT GCACTTGATC TGCTGGACAA AATGCTTGAT 3240

TTAGATCCGG ACAAGCGCAT CACAGCAGAA GACGCGCTCC GGTCACCGTG GCTAAGGAAA 3300

ATCAACCCCG ACGAAATGCC AACGCCACAA TTGCCCACAT GGCAGGACTG CCATGAGCTT 3360

TGGAGTAAGA AGCGCCGTCG GCAGATGCGG GAGCAACAGG AGTCGCTTCC TCCTACGGTG 3420

ATAGCCTCGA CCAAATACCA ACAGCATGGA GCAACCATGG TGGGGGATGC TTAG 3474

Domain Profile

S: 1     yeklekigeGtygkvykakdketgevvAlKkirlekekegvpitalrEisllkelkhkni  60

         +e++++igeGtyg+vyka+d++t+++vAlKk+rle+ekeg+pita+rEi++l++l+h+ni

Q: 804   FEMIAQIGEGTYGQVYKARDHHTNDMVALKKVRLEHEKEGFPITAVREIKILRQLNHRNI  863

         7899********************************************************

S: 61    vkLlevva.........kdkkklyLvfefleqdLkkllkkkkkkklsleevkslllqlle  111

         v+L+e+v+         kdk ++yLvfe++++dL+ ll++    ++++e+  s+++qll+

Q: 864   VNLHEIVTdkqdavefrKDKGSFYLVFEYMDHDLMGLLESGMV-DFNEENNASIMKQLLD  922

         ****************9999*********************99.****************

S: 112   glaylHsnkilHRDlKpqNlLiskegklklaDfGlarafssplk..tytkevvTlwYraP  169

         gl+y+H++++lHRD+K++N+L++++gk+klaDfGlar ++++ +   yt++v+TlwYr+P

Q: 923   GLNYCHKKNFLHRDIKCSNILMNNRGKVKLADFGLARLYNADDRerPYTNKVITLWYRPP  982

         ***************************************88776436*************

S: 170   elLlgakeYstavDiWsvgcilaelltrkplfqgkseidqlerifkllgtpsekvwpevs  229

         elLlg+++Y+ ++D+Ws+gcil el+ ++plfq++ e+ qle+i+k++g+p  +vwp+v

Q: 983   ELLLGEERYGPSIDVWSCGCILGELFVKRPLFQANAEMAQLETISKICGSPVPAVWPNVI  1042

         ************************************************************

S: 230   klpeyk.ksfpkkkkkklkekvkklkekaldllekllaldpkkRisakealqhkyf  284

         klp ++  + +k+++++l+e ++ +   aldll+k+l ldp+kRi+a++al+++++

Q: 1043  KLPLFHtLKQKKTHRRRLREDFEFMPAPALDLLDKMLDLDPDKRITAEDALRSPWL  1098

         ******5566667777******888888************************9996

Domain Sequence
(FASTA)

FEMIAQIGEG TYGQVYKARD HHTNDMVALK KVRLEHEKEG FPITAVREIK ILRQLNHRNI 60

VNLHEIVTDK QDAVEFRKDK GSFYLVFEYM DHDLMGLLES GMVDFNEENN ASIMKQLLDG 120

LNYCHKKNFL HRDIKCSNIL MNNRGKVKLA DFGLARLYNA DDRERPYTNK VITLWYRPPE 180

LLLGEERYGP SIDVWSCGCI LGELFVKRPL FQANAEMAQL ETISKICGSP VPAVWPNVIK 240

LPLFHTLKQK KTHRRRLRED FEFMPAPALD LLDKMLDLDP DKRITAEDAL RSPWL 295

Keyword

ATP-binding; Chromosome; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0000228	; C:nuclear chromosome
GO:0019908	; C:nuclear cyclin-dependent protein kinase holoenzyme complex
GO:0005703	; C:polytene chromosome puff
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0008353	; F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0022008	; P:neurogenesis
GO:0006911	; P:phagocytosis, engulfment
GO:0070816	; P:phosphorylation of RNA polymerase II C-terminal domain

KEGG

dme:Dmel_CG7597

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013