EKS-DRM-00166

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-DRM-00166

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PEK	365.3	2.8E-109	590	1067	478

Status

Reviewed

Ensembl Protein

FBpp0303059

UniProt Accession

Q9NIV1; Q8SX61; Q9VNB8;

Protein Name

Eukaryotic translation initiation factor 2-alpha kinase

Protein Synonyms/Alias

PRKR-like endoplasmic reticulum kinase; DmPEK; PEK; PERK;

Gene Name

PEK

Gene Synonyms/Alias

PEK; EIF2AK3; CG2087;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
FBgn0037327	FBpp0303059	FBtr0330026
FBgn0037327	FBpp0303060	FBtr0330027
FBgn0037327	FBpp0078417	FBtr0078770

Organism

Drosophila melanogaster

Functional Description

Phosphorylates the alpha subunit of eukaryotictranslation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis (By similarity).

Protein Length

1110

Protein Sequence
(FASTA)

MFAPWPTMKT RNQWTRLSSM RLLYISTLDG RLSALDIAKS GKLRWSVPTG PGPLISSSIH 60

RLELTNNGQF VRMIPSLSGG IYKFDGDSID PIPITAEHLL SSSAKFSDDL VISGGKETRS 120

YGVSVRTGQL LYECSLNGCV NSTEEGLAID DTIREPDEED QLEDGEQLRD EAGYIVRHDP 180

LLDDVIIVRR QTQTVRAVES RTGVERWNFS VGQHELDLVR PSECQLQPRD ELELAVLDVD 240

IKVVVPEGII CAFSKSEPQT MLWKYKFDHP IVSAWNTNAD DELQPIDLFS SAQWLWDQDE 300

NDTELPNAPQ SPPSIYLGMY DKQLYIQESI RLRQEIMDQT KVYQQLTGDT SLMPRIPWKP 360

ISASSKSLVI FRKDQEDPEM IAEGAVAQGG ELVPYDDENF AVAAQSVLNA SEFVNGNGFY 420

FYTTGDLNGP QECSTQNNPT DLPAITAPTS PTNATSEGTE ATGNHSVNDD LGFSLDDIDA 480

PVKVVILSLW FWWKEIVVIA FTSAVILNIF MGQRNQRVER EYLVIERHVP VQTAIEATEA 540

STQALLGPVV PMQRPGNRFS FPPGQANQRT ISESTTHSGE HYTSRFQSDF ELMQCLGRGG 600

FGVVFEAKNK LDENRYAIKR ITLPNKESSR QRVLREARTL ASCEHHNIVR YFHSWTETPP 660

TGWQEEEDRK LLAHELSTSI QIETPDDSTM PSLTEQLKEK RQQQLLSWVS DAANSTACSH 720

DFHLPGESSL KNIREEYDYD EEEDSLIEFR SESQSAALRA EEEDDTDDDY EEDEEQQGDH 780

EKRHRSSVSI DIHSASFDLK NINYSQHQLV SNSFQIESVR PKSSGSDDAN DDNKARRKPL 840

TLALAQNHNN NQNGSQPTPS SATILNGTVA KPSKVYLYIQ MQLCRKESLR DWLRDNRSET 900

RAAHIGDIFH QIVDAVDYVH LKGLIHRDLK PSNIFFSQDG QIKIGDFGLV TDMADIPNLV 960

AKCGDQSGLP SCARHTQQVG THLYMSPEQL LGQHYDYKVD IYSLGLIFFE LHVYFSTEME 1020

RIKTLRSLRD GQYPKDFAVN YPQQYDLLQQ MLSAQPEQRP QTKQLKSQLR NILQLPHLLS 1080

EGQSEQAELA ERARRLSRSR TFSSSSEPHQ 1110

Nucleotide Sequence
(FASTA)

ATGTTTGCAC CTTGGCCAAC TATGAAAACC CGTAATCAGT GGACGCGCTT ATCAAGCATG 60

CGACTCCTAT ACATTTCCAC GCTTGATGGT CGACTGAGCG CTCTGGACAT CGCCAAGTCC 120

GGCAAACTGC GCTGGAGTGT GCCCACCGGA CCGGGGCCAC TAATCTCGTC CAGCATCCAT 180

CGATTGGAGC TGACCAACAA TGGGCAGTTC GTACGCATGA TTCCCTCTTT GAGCGGCGGC 240

ATCTACAAGT TCGACGGGGA CTCCATCGAT CCCATTCCAA TCACGGCGGA GCACCTACTC 300

AGCTCGAGCG CCAAATTCAG CGATGATCTG GTTATATCCG GAGGCAAGGA GACACGCTCA 360

TACGGTGTTT CCGTTCGCAC TGGCCAGCTG CTGTATGAAT GTTCTCTAAA CGGATGTGTA 420

AACTCTACGG AGGAAGGGCT TGCAATCGAC GACACCATTA GGGAGCCCGA TGAGGAGGAT 480

CAGCTGGAGG ACGGAGAACA GTTAAGGGAC GAGGCCGGCT ACATAGTGCG CCATGATCCT 540

CTCCTTGACG ACGTTATTAT CGTCCGCCGC CAGACACAAA CGGTTCGGGC CGTGGAATCC 600

CGGACCGGAG TGGAGCGTTG GAACTTTAGC GTAGGTCAGC ATGAGTTGGA TCTGGTGAGA 660

CCTTCCGAGT GCCAGCTACA GCCGCGCGAT GAGCTGGAGC TGGCTGTTTT GGATGTGGAT 720

ATAAAGGTGG TAGTGCCCGA GGGAATTATC TGCGCCTTCA GCAAAAGTGA GCCACAGACT 780

ATGCTGTGGA AGTACAAGTT TGATCACCCA ATTGTGAGTG CCTGGAATAC AAATGCCGAC 840

GACGAATTGC AGCCTATTGA TTTGTTTAGC TCGGCCCAGT GGCTTTGGGA CCAGGACGAA 900

AATGACACGG AGCTGCCCAA TGCGCCACAA AGCCCGCCAT CCATCTACTT GGGCATGTAC 960

GATAAACAGT TGTATATTCA GGAGTCAATA CGTCTACGCC AGGAGATTAT GGACCAGACC 1020

AAGGTCTATC AGCAGCTTAC AGGCGACACC AGCCTGATGC CCAGGATTCC CTGGAAGCCG 1080

ATATCCGCCA GTAGCAAATC GCTGGTAATC TTCCGGAAAG ACCAGGAGGA CCCTGAAATG 1140

ATTGCTGAGG GAGCTGTGGC ACAAGGAGGG GAACTGGTGC CCTATGATGA TGAAAACTTC 1200

GCTGTGGCAG CGCAGTCCGT ATTGAATGCA TCGGAGTTTG TCAATGGCAA TGGTTTTTAC 1260

TTCTACACCA CTGGCGATCT GAACGGCCCG CAAGAGTGCA GTACGCAGAA TAATCCAACT 1320

GACCTTCCAG CAATCACAGC TCCAACATCG CCTACCAATG CCACATCTGA GGGAACGGAA 1380

GCTACTGGAA ACCATAGCGT CAACGACGAT TTGGGCTTTA GTTTGGATGA CATTGATGCG 1440

CCTGTAAAGG TTGTGATATT GTCGTTGTGG TTCTGGTGGA AGGAAATCGT GGTCATAGCA 1500

TTTACGTCGG CTGTGATTCT AAATATTTTC ATGGGCCAAC GAAACCAGCG CGTGGAGCGA 1560

GAGTATCTGG TCATTGAACG TCATGTGCCT GTTCAAACGG CCATCGAGGC CACCGAAGCA 1620

TCCACACAGG CTTTACTAGG TCCAGTGGTG CCGATGCAGC GACCTGGAAA TCGTTTTAGC 1680

TTCCCACCTG GACAAGCAAA TCAGCGCACC ATTTCGGAGT CTACAACTCA TTCAGGCGAG 1740

CATTACACTT CCCGCTTTCA GTCCGACTTC GAGTTGATGC AGTGCCTTGG TCGCGGTGGT 1800

TTCGGAGTGG TCTTCGAAGC TAAGAACAAG CTGGATGAGA ACCGATACGC CATTAAGCGC 1860

ATAACGCTAC CCAATAAGGA ATCTTCAAGA CAGCGAGTCC TTCGCGAGGC TCGAACTTTG 1920

GCTAGTTGTG AGCACCACAA CATCGTGCGA TACTTCCATT CCTGGACCGA GACACCGCCG 1980

ACTGGTTGGC AGGAAGAAGA GGATCGCAAG CTGCTTGCCC ACGAGCTGTC CACCTCTATC 2040

CAGATAGAGA CGCCGGACGA TAGTACAATG CCTTCGTTGA CGGAGCAGTT GAAGGAGAAA 2100

CGCCAACAGC AGCTCTTGTC CTGGGTTTCG GATGCAGCCA ATAGCACCGC TTGTAGTCAC 2160

GACTTTCATC TACCAGGCGA GTCATCACTC AAGAACATAC GCGAGGAGTA CGACTACGAT 2220

GAGGAAGAGG ACTCGCTCAT TGAATTCCGC AGCGAGTCGC AGTCAGCCGC TTTACGGGCA 2280

GAGGAGGAGG ATGATACCGA CGACGACTAT GAAGAGGATG AAGAGCAGCA GGGAGACCAT 2340

GAGAAACGAC ATCGCAGCTC CGTGTCCATT GACATCCATT CGGCCTCGTT TGACCTTAAG 2400

AACATAAACT ACTCACAGCA CCAACTGGTC TCCAATTCGT TTCAAATCGA ATCTGTGCGC 2460

CCCAAGAGCA GTGGAAGTGA TGATGCGAAT GACGATAATA AAGCTAGAAG GAAGCCCCTT 2520

ACCTTGGCAC TGGCCCAGAA TCACAATAAC AACCAAAACG GGAGCCAACC TACGCCCTCC 2580

AGTGCCACGA TTCTGAACGG AACGGTGGCC AAGCCCAGCA AGGTGTACCT CTACATCCAG 2640

ATGCAGCTAT GCCGCAAGGA GAGTCTGCGC GACTGGTTAA GGGACAACCG GTCGGAAACA 2700

AGGGCTGCCC ACATTGGTGA CATTTTTCAC CAGATCGTTG ATGCCGTTGA CTATGTACAT 2760

TTGAAGGGTC TGATTCATCG GGACCTGAAA CCAAGCAACA TATTCTTCTC GCAGGATGGA 2820

CAGATAAAGA TTGGAGACTT CGGGCTCGTC ACCGACATGG CCGATATCCC CAACCTGGTG 2880

GCCAAGTGTG GGGACCAGAG TGGCCTGCCC TCCTGTGCCC GCCACACCCA GCAGGTGGGC 2940

ACCCACTTAT ACATGTCGCC GGAGCAGCTT TTGGGCCAGC ACTACGACTA CAAGGTGGAC 3000

ATCTATTCAC TGGGACTGAT CTTCTTCGAG CTGCACGTGT ACTTCAGCAC TGAAATGGAG 3060

CGGATCAAGA CACTGCGCAG TCTTCGGGAC GGCCAGTATC CCAAGGATTT CGCTGTCAAT 3120

TACCCGCAGC AGTACGACTT GCTCCAGCAA ATGCTATCCG CCCAGCCAGA GCAACGCCCG 3180

CAGACCAAGC AGTTGAAAAG CCAGCTGCGC AACATTCTGC AGCTACCTCA TCTCCTCTCG 3240

GAAGGACAAA GCGAGCAGGC GGAGCTGGCG GAGCGGGCCA GGCGCCTGAG TCGCAGCCGC 3300

ACCTTCAGCA GCTCCAGTGA GCCGCATCAG TGA 3333

Domain Profile

S: 1     feeikvlGkGgfgvVlkaknkldkkeyaikriklnkakkelekverevkaLaklehpniv  60

         fe +++lG+GgfgvV++aknkld+++yaikri+l+++++++++v+re+++La++eh+niv

Q: 590   FELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNIV  649

         7889********************************************************

S: 61    rYfaawleekeesl..............................................  74

         rYf++w+e++++++

Q: 650   RYFHSWTETPPTGWqeeedrkllahelstsiqietpddstmpslteqlkekrqqqllswv  709

         **************9999999999999999999999998888888888888888888888

S: 75    .....................................................tdsseve  81

Q: 710   sdaanstacshdfhlpgesslknireeydydeeedsliefrsesqsaalraeeE------  763

         888888888888888888888888888888877655555554444333333330......

S: 82    aeaeleesesseesdtk...........................................  98

                      +dt+

Q: 764   -------------DDTDddyeedeeqqgdhekrhrssvsidihsasfdlkninysqhqlv  810

         .............3333555555556666777777778899999999999**********

S: 99    ............................................................  98

Q: 811   snsfqiesvrpkssgsddanddnkarrkpltlalaqnhnnnqngsqptpssatilngtva  870

         ************************************************************

S: 99    .....llyiqmelceketLkdwiderkllesrselvmrlfkqllegleylHskgliHrdL  153

              +lyiqm+lc+ke+L+dw++++ ++e+r  ++ ++f+q++++++y+H kgliHrdL

Q: 871   kpskvYLYIQMQLCRKESLRDWLRDN-RSETRAAHIGDIFHQIVDAVDYVHLKGLIHRDL  929

         **************************.5666666666***********************

S: 154   kpsniFldsddqvKigDFgLatalaealkedvkqdaqske...akhtgqvgtklymsPeq  210

         kpsniF+++d+q+KigDFgL+t++a++ +  +k+ +qs+    a+ht+qvgt+lymsPeq

Q: 930   KPSNIFFSQDGQIKIGDFGLVTDMADIPNLVAKCGDQSGLpscARHTQQVGTHLYMSPEQ  989

         ******************************99999999987778****************

S: 211   leksqYnkkvDifsLGlilfeLlypfstemervetltelrdgkipedfddkvpkekkllq  270

         l +++Y+ kvDi+sLGli+feL++ fstemer++tl++lrdg++p+df+ ++p++++llq

Q: 990   LLGQHYDYKVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQ  1049

         ************************************************************

S: 271   kllsrdpkeRpsasellk  288

         ++ls +p++Rp++++l +

Q: 1050  QMLSAQPEQRPQTKQLKS  1067

         ***************976

Domain Sequence
(FASTA)

FELMQCLGRG GFGVVFEAKN KLDENRYAIK RITLPNKESS RQRVLREART LASCEHHNIV 60

RYFHSWTETP PTGWQEEEDR KLLAHELSTS IQIETPDDST MPSLTEQLKE KRQQQLLSWV 120

SDAANSTACS HDFHLPGESS LKNIREEYDY DEEEDSLIEF RSESQSAALR AEEEDDTDDD 180

YEEDEEQQGD HEKRHRSSVS IDIHSASFDL KNINYSQHQL VSNSFQIESV RPKSSGSDDA 240

NDDNKARRKP LTLALAQNHN NNQNGSQPTP SSATILNGTV AKPSKVYLYI QMQLCRKESL 300

RDWLRDNRSE TRAAHIGDIF HQIVDAVDYV HLKGLIHRDL KPSNIFFSQD GQIKIGDFGL 360

VTDMADIPNL VAKCGDQSGL PSCARHTQQV GTHLYMSPEQ LLGQHYDYKV DIYSLGLIFF 420

ELHVYFSTEM ERIKTLRSLR DGQYPKDFAV NYPQQYDLLQ QMLSAQPEQR PQTKQLKS 478

Keyword

ATP-binding; Complete proteome; Endoplasmic reticulum; Glycoprotein; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Signal; Stress response; Transferase; Translation regulation; Transmembrane; Transmembrane helix; Unfolded protein response.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005789	; C:endoplasmic reticulum membrane
GO:0016021	; C:integral to membrane
GO:0005524	; F:ATP binding
GO:0004686	; F:elongation factor-2 kinase activity
GO:0010389	; P:regulation of G2/M transition of mitotic cell cycle
GO:0006417	; P:regulation of translation
GO:0006986	; P:response to unfolded protein

KEGG

dme:Dmel_CG2087

;

InterPros

IPR011009	; Kinase-like_dom.
IPR018391	; PQQ_beta_propeller_repeat.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR011047	; Quinonprotein_ADH-like.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 2.

SMARTs

SM00564

; PQQ; 2.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013