EKS-ORS-00640

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-ORS-00640

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	321.2	1.4E-95	13	268	256

Status

Reviewed

Ensembl Protein

LOC_Os12g03810.2

UniProt Accession

Q2QY53;

Protein Name

CBL-interacting protein kinase 32

Protein Synonyms/Alias

OsCIPK32;

Gene Name

CIPK32

Gene Synonyms/Alias

CIPK32; Os12g0132200, LOC_Os12g03810;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
LOC_Os12g03810	LOC_Os12g03810.3	LOC_Os12g03810.3
LOC_Os12g03810	LOC_Os12g03810.1	LOC_Os12g03810.1
LOC_Os12g03810	LOC_Os12g03810.2	LOC_Os12g03810.2

Organism

Oryza sativa

Functional Description

CIPK serine-threonine protein kinases interact with CBLproteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium- dependent manner (By similarity).

Protein Length

438

Protein Sequence
(FASTA)

MSTTKVKRRV GKYELGRTIG EGTFAKVKFA RDTETGDPVA IKILDKEKVL KHKMVEQIKR 60

EISTMKLIKH PNVVRIYEVM GSKTKIYIVL EYVTGGELFD TIVNHGRMRE DEARRYFQQL 120

INAVDYCHSR GVYHRDLKPE NLLLDSYGNL KVSDFGLSAL SQQIKDDGLL HTTCGTPNYV 180

APEVLEDQGY DGAMADLWSC GVILFVLLAG YLPFEDSNLM TLYKKISNAE FTFPPWTSFP 240

AKRLLTRILD PNPMTRVTIP EILEDEWFKK GYKRPEFDEK YDTTLDDVYA VFNDSEEHHV 300

TEKKEEPEAL NAFELISMSA GLNLGNLFDS EQEFKRETRF TSKCPPKEIV RKIEEAAKPL 360

GFDVQKKNYK LRLEKVKAGR KGNLNVATEI LQVAPSLHMV EVRKAKGDTL EFHKFYKNLS 420

RTLKDVVWKS DDLQNQLS 438

Nucleotide Sequence
(FASTA)

ATGAGTACAA CCAAAGTGAA GAGACGTGTG GGAAAGTATG AGCTAGGAAG AACAATCGGA 60

GAGGGCACAT TCGCGAAGGT CAAGTTCGCG AGGGATACCG AGACCGGTGA TCCAGTAGCC 120

ATCAAGATCC TAGATAAGGA GAAAGTTCTC AAGCATAAGA TGGTCGAACA GATTAAAAGG 180

GAAATTTCGA CAATGAAGTT GATTAAGCAC CCAAATGTTG TCCGTATATA TGAGGTGATG 240

GGAAGCAAGA CAAAAATCTA CATTGTTTTA GAATATGTTA CCGGTGGTGA GCTCTTTGAT 300

ACAATTGTTA ACCATGGTCG TATGAGGGAA GATGAGGCAA GAAGGTACTT CCAACAATTA 360

ATTAATGCTG TTGATTATTG TCATAGCAGG GGTGTGTACC ACAGGGACTT AAAACCAGAA 420

AATTTACTAC TAGATTCATA TGGGAATCTG AAGGTCTCTG ATTTTGGGCT GAGTGCACTT 480

TCTCAGCAAA TTAAGGATGA TGGTTTGCTG CACACAACCT GCGGGACACC AAATTATGTT 540

GCACCGGAGG TCCTTGAAGA TCAAGGATAT GATGGAGCAA TGGCAGATTT GTGGTCATGT 600

GGAGTTATCC TGTTCGTTCT GCTAGCAGGG TATTTGCCTT TTGAGGACTC TAATCTTATG 660

ACGTTGTATA AGAAAATATC AAATGCAGAG TTTACGTTTC CACCTTGGAC GTCTTTTCCT 720

GCCAAGAGGT TGTTAACAAG AATCCTTGAT CCAAACCCAA TGACGAGAGT AACAATTCCA 780

GAAATATTAG AGGATGAGTG GTTCAAAAAG GGCTACAAAC GCCCAGAGTT TGACGAAAAA 840

TATGACACAA CATTGGATGA TGTTTATGCT GTCTTCAATG ACTCTGAAGA GCACCATGTG 900

ACAGAAAAGA AAGAAGAACC AGAAGCTCTC AATGCATTTG AACTGATTTC AATGTCAGCA 960

GGCCTCAATC TTGGGAATTT GTTTGATTCA GAGCAGGAAT TCAAAAGAGA AACAAGGTTC 1020

ACATCAAAAT GTCCACCAAA AGAAATTGTC CGCAAGATTG AGGAAGCCGC AAAACCTCTA 1080

GGATTTGATG TTCAGAAGAA AAATTACAAG TTAAGGTTGG AAAAGGTAAA AGCAGGGAGA 1140

AAAGGAAATC TCAATGTTGC TACTGAGATA CTGCAGGTTG CACCCTCTCT TCATATGGTT 1200

GAAGTACGAA AAGCAAAAGG CGACACCCTC GAATTTCATA AGTTCTACAA GAACCTCTCC 1260

AGAACCTTAA AGGATGTTGT TTGGAAATCA GATGATCTTC AAAATCAACT TTCCTAG 1317

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl.seeslekikrEieilkklkhpn  59

         y+l++t+Geg+f+kVk a+++et+  va+Ki++k+k+ +++ +e+ikrEi+++k +khpn

Q: 13    YELGRTIGEGTFAKVKFARDTETGDPVAIKILDKEKVlKHKMVEQIKREISTMKLIKHPN  72

         99********************************9987889999****************

S: 60    iikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgi  119

         +++++ev+ +k+k+y+vley++ggeLfd+i+++gr++e+ear++f+ql++av+Y+hs+g+

Q: 73    VVRIYEVMGSKTKIYIVLEYVTGGELFDTIVNHGRMREDEARRYFQQLINAVDYCHSRGV  132

         ************************************************************

S: 120   vHrDlKpeNllldkkenikiaDFGlan...lfkegklletfcGsppYaaPellkgkkYeg  176

          HrDlKpeNllld+ +n+k++DFGl++   ++k++ ll+t+cG+p+Y+aPe+l+++ Y+g

Q: 133   YHRDLKPENLLLDSYGNLKVSDFGLSAlsqQIKDDGLLHTTCGTPNYVAPEVLEDQGYDG  192

         ************************99866678888*************************

S: 177   pkvDvWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvd  236

           +D+Ws+Gv+L++l++g lPf+++nl +l++ki++ ++++p + s  +++Ll+++L  +

Q: 193   AMADLWSCGVILFVLLAGYLPFEDSNLMTLYKKISNAEFTFPPWTSFPAKRLLTRILDPN  252

         ************************************************************

S: 237   pekRatieeilkhewl  252

         p +R+ti eil++ew+

Q: 253   PMTRVTIPEILEDEWF  268

         ***************7

Domain Sequence
(FASTA)

YELGRTIGEG TFAKVKFARD TETGDPVAIK ILDKEKVLKH KMVEQIKREI STMKLIKHPN 60

VVRIYEVMGS KTKIYIVLEY VTGGELFDTI VNHGRMREDE ARRYFQQLIN AVDYCHSRGV 120

YHRDLKPENL LLDSYGNLKV SDFGLSALSQ QIKDDGLLHT TCGTPNYVAP EVLEDQGYDG 180

AMADLWSCGV ILFVLLAGYL PFEDSNLMTL YKKISNAEFT FPPWTSFPAK RLLTRILDPN 240

PMTRVTIPEI LEDEWF 256

Keyword

ATP-binding; Complete proteome; Kinase; Manganese; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Arabidopsis lyrata"; ?>Arabidopsis thaliana"; ?>Brassica rapa"; ?>Glycine max"; ?>Hordeum vulgare"; ?>Musa acuminata"; ?>Oryza brachyantha"; ?>Oryza indica"; ?>Populus trichocarpa"; ?>Setaria italica"; ?>Solanum lycopersicum"; ?>Solanum tuberosum"; ?>Sorghum bicolor"; ?>Vitis vinifera"; ?>Zea mays"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0009628	; P:response to abiotic stimulus
GO:0009737	; P:response to abscisic acid stimulus
GO:0009735	; P:response to cytokinin stimulus
GO:0007165	; P:signal transduction

KEGG

dosa:Os12t0132200-01	;
osa:4351419	;

InterPros

IPR020636	; Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009	; Kinase-like_dom.
IPR018451	; NAF/FISL_domain.
IPR004041	; NAF_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF03822	; NAF; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50816	; NAF; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013