EKS-SCP-00027

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SCP-00027

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PLK	344.3	1.1E-103	41	296	256

Status

Reviewed

Ensembl Protein

SPAC23C11.16.1:pep

UniProt Accession

P50528;

Protein Name

Serine/threonine-protein kinase plo1

Protein Synonyms/Alias

Gene Name

plo1

Gene Synonyms/Alias

plo1; SPAC23C11.16;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
SPAC23C11.16	SPAC23C11.16.1:pep	SPAC23C11.16.1

Organism

Schizosaccharomyces pombe

Functional Description

Required to form a bipolar spindle, the actin ring andseptum. Functions upstream of the whole septum formation pathway, including actin ring formation (regulated by late septation genes) and septal material deposition (regulated by early septation genes). Behaves as a "septum-promoting factor", and could also be involved in inducing other late events of cell division.

Protein Length

683

Protein Sequence
(FASTA)

MASVAIKEPK TAITPKKKSK ASRLCFTPPT NLHNNKKNIF YTRYDCIGEG GFARCFRVKD 60

NYGNIYAAKV IAKRSLQNDK TKLKLFGEIK VHQSMSHPNI VGFIDCFEDS TNIYLILELC 120

EHKSLMELLR KRKQLTEPEV RYLMMQILGA LKYMHKKRVI HRDLKLGNIM LDESNNVKIG 180

DFGLAALLMD DEERKMTICG TPNYIAPEIL FNSKEGHSFE VDLWSAGVVM YALLIGKPPF 240

QDKEVKTIYR KIKANSYSFP SNVDISAEAK DLISSLLTHD PSIRPSIDDI VDHEFFHTGY 300

MASTLPDEIL HSMPIWPSSQ SKSSFQRNLD FVASASGVGF GNSAGVEKNK PYALRTDEVD 360

NDRILPSVLS PRDRVNPVMK IGPETKPVPS KLSTALHAAR KSTDGSLGSR VKVLREESQS 420

FVPTKSAVTE QVEPIQLIRS LSANTVSRLS KVGNMKSDIW ISVKKTALKI GMALEAHTHA 480

LTSEDADSEP VLFITKWVDY SNKYGLGYQL SDESVGVHFN DDTSLLFSAD EEVVEYALHP 540

KDTEIKPYIY PASKVPESIR SKLQLLKHFK SYMGQNLSKA VQDESFEKPK NSTSNTMLFM 600

QHYLRTRQAI MFRLSNGIFQ FNFLDHRKVV ISSTARKIIV LDKERERVEL PLQEASAFSE 660

DLRSRLKYIR ETLESWASKM EVS 683

Nucleotide Sequence
(FASTA)

ATGGCGAGTG TTGCAATTAA AGAGCCCAAA ACTGCTATAA CCCCGAAGAA GAAGTCCAAA 60

GCCTCTCGTC TTTGCTTTAC TCCGCCAACT AATCTTCATA ATAATAAAAA AAATATATTC 120

TACACTCGCT ACGATTGCAT TGGTGAAGGC GGCTTTGCGA GATGTTTTCG AGTCAAAGAT 180

AATTATGGAA ATATTTACGC TGCTAAAGTT ATTGCTAAAA GGTCACTACA AAATGATAAA 240

ACAAAGCTGA AGCTCTTTGG AGAAATAAAG GTGCACCAAT CCATGAGTCA TCCAAACATC 300

GTAGGATTTA TAGATTGTTT TGAAGATTCG ACGAATATTT ACTTAATTTT GGAGCTTTGT 360

GAACATAAAT CTTTAATGGA ACTTTTACGT AAGCGAAAAC AATTAACTGA GCCAGAGGTT 420

CGGTATTTAA TGATGCAAAT TTTGGGAGCT TTGAAATATA TGCATAAAAA GAGAGTCATT 480

CATAGAGATT TAAAGCTTGG AAATATTATG CTTGATGAGA GTAACAATGT AAAAATTGGT 540

GATTTTGGGC TTGCCGCGCT TTTAATGGAT GACGAAGAAC GTAAAATGAC TATCTGTGGT 600

ACACCGAACT ACATCGCACC TGAAATTTTG TTCAATTCAA AAGAAGGACA TTCATTTGAA 660

GTTGATCTTT GGAGTGCTGG AGTAGTCATG TATGCCCTCT TGATTGGAAA GCCACCCTTC 720

CAGGATAAAG AGGTAAAGAC AATTTATAGG AAGATAAAAG CCAACTCATA TAGTTTTCCT 780

TCAAATGTCG ACATATCTGC AGAAGCAAAA GATTTAATTT CTTCTCTTCT GACACATGAT 840

CCAAGCATTC GTCCCAGTAT TGACGATATT GTTGACCATG AATTCTTTCA CACTGGCTAT 900

ATGGCTTCTA CACTTCCAGA TGAAATTTTA CATTCAATGC CTATATGGCC TTCTTCCCAA 960

TCAAAATCAA GCTTTCAGAG AAACCTGGAC TTCGTTGCCT CTGCTTCCGG AGTAGGCTTT 1020

GGAAATTCAG CAGGTGTAGA AAAGAATAAA CCTTATGCAC TTCGAACAGA TGAAGTTGAT 1080

AATGATCGAA TATTACCAAG TGTTTTAAGT CCAAGGGACC GTGTCAACCC TGTTATGAAA 1140

ATAGGACCGG AAACCAAACC TGTACCATCA AAGCTCTCTA CAGCCCTCCA TGCAGCTCGT 1200

AAATCCACTG ATGGAAGTTT AGGCTCAAGA GTTAAAGTTT TGAGAGAAGA ATCACAATCA 1260

TTTGTTCCAA CTAAGAGCGC TGTCACTGAA CAAGTTGAAC CTATTCAATT AATTCGGTCT 1320

TTATCTGCAA ATACAGTTTC AAGACTTTCT AAGGTTGGAA ATATGAAATC AGACATTTGG 1380

ATCAGCGTAA AGAAGACAGC TCTTAAAATC GGGATGGCAT TGGAAGCACA TACACACGCT 1440

TTAACTTCAG AGGATGCTGA CTCTGAGCCC GTTCTTTTTA TTACAAAGTG GGTTGATTAT 1500

TCCAACAAAT ATGGTTTAGG ATACCAGCTA TCCGATGAAT CGGTTGGCGT ACACTTTAAT 1560

GATGATACAT CCTTGCTTTT TTCAGCTGAT GAGGAGGTTG TCGAGTATGC TTTACATCCT 1620

AAAGATACAG AAATTAAGCC ATATATTTAT CCTGCTTCCA AAGTTCCTGA ATCTATAAGA 1680

AGTAAACTTC AGCTTTTAAA GCACTTTAAA AGCTATATGG GACAAAATCT TTCGAAGGCT 1740

GTGCAGGATG AGAGTTTTGA AAAACCCAAA AACTCAACTT CCAATACCAT GCTTTTCATG 1800

CAGCATTATC TTCGTACGCG TCAAGCTATA ATGTTTAGAT TAAGTAACGG AATTTTTCAG 1860

TTCAATTTCC TGGATCATCG AAAAGTTGTT ATTTCCTCTA CCGCACGGAA GATCATTGTA 1920

TTAGATAAAG AGCGAGAGAG AGTGGAATTG CCTTTGCAAG AAGCATCCGC ATTTTCAGAA 1980

GACTTGAGAT CTCGCTTAAA GTATATTCGC GAGACGTTGG AATCGTGGGC GTCGAAAATG 2040

GAAGTGAGTT AA 2052

Domain Profile

S: 1     yergkllGkGgfakcyelkdletkevfavkvvpksllakekqrekvekeieihrslkhkn  60

         y+r   +G+Ggfa+c+++kd + ++++a+kv++k +l++ k++ k+  ei++h+s++h+n

Q: 41    YTRYDCIGEGGFARCFRVKD-NYGNIYAAKVIAKRSLQNDKTKLKLFGEIKVHQSMSHPN  99

         788999*************9.8899***********************************

S: 61    ivklyeffedkenvylvlelcsrrslaellkrrkaltepevryylrqivsglkylhekri  120

         iv ++++fed+ n+yl+lelc+++sl+ell++rk+ltepevry + qi+ +lky+h+kr+

Q: 100   IVGFIDCFEDSTNIYLILELCEHKSLMELLRKRKQLTEPEVRYLMMQILGALKYMHKKRV  159

         ************************************************************

S: 121   lhrdlklgnlflneelevkigdfGlatrlekeeerkktlcGtPnyiaPevl..skkghsl  178

         +hrdlklgn++l+e+ +vkigdfGla+ l  +eerk+t+cGtPnyiaPe+l  sk+ghs+

Q: 160   IHRDLKLGNIMLDESNNVKIGDFGLAALLMDDEERKMTICGTPNYIAPEILfnSKEGHSF  219

         **************************************************98899*****

S: 179   evdiWslGcilytllvGkPPfetkelketyekikkaeyslPss..lskeaksliakllkk  236

         evd+Ws G+++y+ll+GkPPf+ ke+k++y+kik ++ys+Ps+  +s+eak+li++ll++

Q: 220   EVDLWSAGVVMYALLIGKPPFQDKEVKTIYRKIKANSYSFPSNvdISAEAKDLISSLLTH  279

         *****************************************9866899************

S: 237   ePeerpsleevlkdefl  253

         +P+ rps+++++++ef+

Q: 280   DPSIRPSIDDIVDHEFF  296

         ****************8

Domain Sequence
(FASTA)

YTRYDCIGEG GFARCFRVKD NYGNIYAAKV IAKRSLQNDK TKLKLFGEIK VHQSMSHPNI 60

VGFIDCFEDS TNIYLILELC EHKSLMELLR KRKQLTEPEV RYLMMQILGA LKYMHKKRVI 120

HRDLKLGNIM LDESNNVKIG DFGLAALLMD DEERKMTICG TPNYIAPEIL FNSKEGHSFE 180

VDLWSAGVVM YALLIGKPPF QDKEVKTIYR KIKANSYSFP SNVDISAEAK DLISSLLTHD 240

PSIRPSIDDI VDHEFF 256

Keyword

ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Mitosis; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005816	; C:spindle pole body
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0034605	; P:cellular response to heat
GO:0000915	; P:cytokinesis, actomyosin contractile ring assembly
GO:0031031	; P:positive regulation of septation initiation signaling cascade
GO:0010687	; P:site selection involved in cell cycle cytokinesis
GO:0090307	; P:spindle assembly involved in mitosis
GO:0007089	; P:traversing start control point of mitotic cell cycle

KEGG

spo:SPAC23C11.16

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000959	; POLO_box_duplicated_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00659	; POLO_box; 2.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS50078	; POLO_BOX; 2.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013