EKS-SCP-00003

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SCP-00003

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE11	317.4	2.2E-95	394	658	265

Status

Reviewed

Ensembl Protein

SPBC1D7.05.1:pep

UniProt Accession

P28829; Q7LGJ0;

Protein Name

Protein kinase byr2

Protein Synonyms/Alias

MAPK kinase kinase; MAPKKK; Protein kinase ste8;

Gene Name

byr2

Gene Synonyms/Alias

byr2; ste8; SPBC1D7.05, SPBC2F12.01;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
SPBC1D7.05	SPBC1D7.05.1:pep	SPBC1D7.05.1

Organism

Schizosaccharomyces pombe

Functional Description

Serine/threonine protein kinase involved in conjugationand sporulation. It is thought that it phosphorylates the byr1 protein kinase which itself phosphorylate the spk1 kinase.

Protein Length

659

Protein Sequence
(FASTA)

MEYYTSKEVA EWLKSIGLEK YIEQFSQNNI EGRHLNHLTL PLLKDLGIEN TAKGKQFLKQ 60

RDYLREFPRP CILRFIACNG QTRAVQSRGD YQKTLAIALK KFSLEDASKF IVCVSQSSRI 120

KLITEEEFKQ ICFNSSSPER DRLIIVPKEK PCPSFEDLRR SWEIELAQPA ALSSQSSLSP 180

KLSSVLPTST QKRSVRSNNA KPFESYQRPP SELINSRISD FFPDHQPKLL EKTISNSLRR 240

NLSIRTSQGH NLGNFGQEIL PRSSRRARPS ELVCPLSSLR ISVAEDVNRL PRIDRGFDPP 300

LTVSSTQRIS RPPSLQKSIT MVGVEPLYQS NGNEKSSKYN VFSESAHGNH QVLSFSPGSS 360

PSFIEQPSPI SPTSTTSEDT NTLEEDTDDQ SIKWIRGALI GSGSFGQVYL GMNASSGELM 420

AVKQVILDSV SESKDRHAKL LDALAGEIAL LQELSHEHIV QYLGSNLNSD HLNIFLEYVP 480

GGSVAGLLTM YGSFEETLVK NFIKQTLKGL EYLHSRGIVH RDIKGANILV DNKGKIKISD 540

FGISKKLELN STSTKTGGAR PSFQGSSFWM APEVVKQTMH TEKTDIWSLG CLVIEMLTSK 600

HPYPNCDQMQ AIFRIGENIL PEFPSNISSS AIDFLEKTFA IDCNLRPTAS ELLSHPFVS 659

Nucleotide Sequence
(FASTA)

ATGGAATATT ATACCTCGAA GGAGGTAGCA GAATGGTTGA AAAGTATCGG ATTGGAGAAG 60

TATATCGAAC AATTTTCACA AAATAACATT GAAGGACGAC ACCTAAATCA TTTAACCTTG 120

CCTTTACTGA AAGACCTAGG CATTGAAAAC ACCGCCAAAG GCAAACAATT TTTAAAACAA 180

AGAGACTATT TACGAGAGTT TCCACGTCCA TGTATACTGA GGTTTATTGC CTGTAACGGG 240

CAAACTCGAG CAGTTCAATC ACGAGGAGAT TACCAGAAAA CACTCGCTAT TGCTTTGAAA 300

AAGTTTAGCC TTGAAGATGC TTCAAAATTC ATTGTATGCG TTTCACAGAG TAGTCGAATT 360

AAGCTGATTA CTGAAGAAGA ATTTAAACAA ATTTGTTTTA ATTCATCTTC ACCGGAACGC 420

GACAGGTTAA TTATTGTGCC AAAAGAAAAG CCTTGTCCAT CGTTTGAAGA CCTCCGCCGT 480

TCTTGGGAGA TTGAATTGGC TCAACCGGCA GCATTATCAT CACAGTCCTC CCTTTCTCCT 540

AAACTTTCCT CTGTTCTTCC CACGAGCACT CAGAAACGAA GTGTCCGCTC AAATAATGCG 600

AAACCATTTG AATCCTACCA GCGACCTCCT AGCGAGCTTA TTAATTCTAG AATTTCCGAT 660

TTTTTCCCCG ATCATCAACC AAAGCTACTG GAAAAAACAA TATCAAACTC TCTTCGTAGA 720

AACCTTAGCA TACGTACGTC TCAAGGGCAC AATCTTGGTA ATTTTGGTCA AGAAATTTTG 780

CCAAGAAGCT CTCGGCGGGC TCGCCCTTCC GAATTGGTTT GTCCTCTTTC CTCTCTGAGG 840

ATTTCTGTTG CTGAAGATGT TAACCGACTT CCTAGGATTG ATAGGGGATT TGATCCTCCA 900

CTTACTGTAT CTTCTACTCA GCGTATTTCT AGACCACCAT CGCTTCAAAA ATCCATAACC 960

ATGGTTGGTG TGGAACCTTT ATACCAGTCC AACGGAAATG AAAAATCGTC GAAGTATAAT 1020

GTTTTTTCAG AAAGCGCGCA TGGCAACCAT CAAGTACTTT CCTTTTCACC TGGTTCATCC 1080

CCATCATTTA TTGAGCAACC ATCCCCAATT TCTCCAACTT CCACTACAAG TGAGGACACA 1140

AATACACTTG AAGAGGACAC TGATGATCAG TCTATCAAAT GGATACGTGG TGCGTTAATA 1200

GGTTCAGGAT CGTTTGGACA AGTTTATCTC GGTATGAATG CATCTAGTGG TGAGTTGATG 1260

GCTGTTAAGC AAGTGATCTT AGATTCTGTT TCTGAATCAA AAGACCGCCA TGCCAAACTT 1320

TTAGATGCAT TAGCGGGTGA AATTGCTCTT CTACAGGAGT TGTCTCATGA GCATATTGTC 1380

CAATATCTCG GTTCTAACTT AAATTCGGAC CACTTGAACA TTTTTTTGGA ATATGTTCCT 1440

GGGGGGTCGG TTGCCGGATT GCTTACAATG TATGGAAGCT TTGAGGAAAC GCTAGTCAAG 1500

AACTTTATTA AGCAAACCCT TAAAGGCTTA GAATACCTTC ATTCACGCGG CATTGTACAC 1560

CGTGATATAA AAGGTGCGAA CATCTTGGTT GATAATAAAG GGAAGATTAA AATTTCGGAC 1620

TTTGGGATAT CGAAGAAACT TGAACTAAAT AGTACCTCTA CGAAGACGGG TGGTGCTCGC 1680

CCTTCTTTTC AAGGCTCTTC TTTTTGGATG GCTCCCGAAG TGGTGAAGCA AACTATGCAT 1740

ACGGAGAAAA CAGATATTTG GTCTTTGGGA TGTTTGGTAA TCGAGATGCT CACTAGTAAG 1800

CACCCTTATC CGAATTGTGA TCAAATGCAG GCCATTTTCA GAATTGGGGA GAACATTCTG 1860

CCGGAATTCC CTTCTAACAT TTCTTCATCG GCCATAGATT TCCTTGAGAA AACGTTTGCC 1920

ATAGATTGTA ATCTTAGACC TACAGCATCA GAGTTGTTGA GCCACCCGTT CGTTTCATAA 1980

Domain Profile

S: 1     wqkrevigkGsfgsvyaaldvdtgellavKeveik...pekksekkevkalkeEikllke  57

         w ++++ig+Gsfg+vy+++++++gel+avK+v ++   + k+++ k ++al+ Ei+ll+e

Q: 394   WIRGALIGSGSFGQVYLGMNASSGELMAVKQVILDsvsESKDRHAKLLDALAGEIALLQE  453

         78999***************************8873333344445599************

S: 58    lkHenivkyyGveveeeelkiflEyvegGslssllekygkleesvikkytkqileglayL  117

         l+He iv+y+G++ ++++l+iflEyv+gGs++ ll++yg++ee+++k++ kq l+gl+yL

Q: 454   LSHEHIVQYLGSNLNSDHLNIFLEYVPGGSVAGLLTMYGSFEETLVKNFIKQTLKGLEYL  513

         ************************************************************

S: 118   HekkivHRDiKgaNilldssgvvklsDFGaskrlaelntkaae...lesltGtlaymaPE  174

         H+++ivHRDiKgaNil+d++g +k+sDFG+sk+l+ ++t++++   + s++G+ ++maPE

Q: 514   HSRGIVHRDIKGANILVDNKGKIKISDFGISKKLELNSTSTKTggaRPSFQGSSFWMAPE  573

         **********************************98877755566688************

S: 175   vikkkekgygrkaDiWslgcvviemltgkrPfaeldeelaimfkvgakktkPeipeklse  234

         v+k++   +++k+DiWslgc viemlt+k+P+ ++ ++++++f++g +   Pe+p+++s+

Q: 574   VVKQT--MHTEKTDIWSLGCLVIEMLTSKHPYPNC-DQMQAIFRIGENI-LPEFPSNISS  629

         **998..679*************************.9*********998.**********

S: 235   egkdflekcleldpkkrpsaeeLLkhefl  263

         ++ dflek++ +d + rp+a+eLL+h+f+

Q: 630   SAIDFLEKTFAIDCNLRPTASELLSHPFV  658

         ***************************96

Domain Sequence
(FASTA)

WIRGALIGSG SFGQVYLGMN ASSGELMAVK QVILDSVSES KDRHAKLLDA LAGEIALLQE 60

LSHEHIVQYL GSNLNSDHLN IFLEYVPGGS VAGLLTMYGS FEETLVKNFI KQTLKGLEYL 120

HSRGIVHRDI KGANILVDNK GKIKISDFGI SKKLELNSTS TKTGGARPSF QGSSFWMAPE 180

VVKQTMHTEK TDIWSLGCLV IEMLTSKHPY PNCDQMQAIF RIGENILPEF PSNISSSAID 240

FLEKTFAIDC NLRPTASELL SHPFV 265

Keyword

3D-structure; ATP-binding; Complete proteome; Conjugation; Kinase; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Sporulation; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0004709	; F:MAP kinase kinase kinase activity
GO:0000751	; P:cell cycle arrest in response to pheromone
GO:0031142	; P:induction of conjugation upon nitrogen starvation
GO:0032005	; P:signal transduction involved in conjugation with cellular fusion
GO:0030435	; P:sporulation resulting in formation of a cellular spore

KEGG

spo:SPBC1D7.05

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR001660	; SAM.
IPR013761	; SAM/pointed.
IPR011510	; SAM_2.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF07647	; SAM_2; 1.

SMARTs

SM00220	; S_TKc; 1.
SM00454	; SAM; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50105	; SAM_DOMAIN; 1.

Prints

Created Date

20-Feb-2013