EKS-SCP-00043

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SCP-00043

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/CDC7	289.9	6.0E-87	68	432	365

Status

Reviewed

Ensembl Protein

SPBC776.12c.1:pep

UniProt Accession

P50582; O94678;

Protein Name

Cell cycle serine/threonine-protein kinase hsk1

Protein Synonyms/Alias

Cdc7-related kinase; Minichromosome maintenance protein kinase;

Gene Name

hsk1

Gene Synonyms/Alias

hsk1; SPBC776.12c;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
SPBC776.12c	SPBC776.12c.1:pep	SPBC776.12c.1

Organism

Schizosaccharomyces pombe

Functional Description

Required for G1/S transition. Plays a role in DNAreplication checkpoint signaling through regulating rad3 and cds1. Involved in the maintenance of mitotic chromosome structures during S phase through regulating the function of rad21. Required for initiation of mitotic DNA replication through phosphorylating mcm2/cdc19. Required for genome integrity.

Protein Length

507

Protein Sequence
(FASTA)

MAEAHITLSP KVTHEQQTDI DSECEITEVD DENVNENKSQ EMIQDIPARD REEIENITRT 60

FVELQENYRL IEKIGEGTFS SVYKAEDLHY GRYINDWDIQ SEVLKESSFG KEKIPVNEDS 120

RKPKYVAIKK IYATSSPARI YNELEILYLL RGSSVIAPLI TALRNEDQVL VVLPYYEHTD 180

FRQYYSTFSY RDMSIYFRCL FQAMQQTQTL GIIHRDIKPS NFLFDVRTKH GVLVDFGLAE 240

RYDGRQQSHS CRCTNSNAAE LAHDFSIAQE TSLGYIKNDT RPSKRANRAG TRGFRAPEVL 300

FKCSSQSPKV DIWSAGVILL SFLTKRFPMF NSKDDVDALM EIACIFGKSE MRQCAALHGC 360

TFETNVSTLT EKRVNFRKLI LWASCGSASI YKEKLRHKPS QEERLCLDFL EKCLELDCNK 420

RISAEEALDH DFLYLDNLAY EKKDDDTAFD NSFGETSFEK DEDLTAKHLS HILDFKEQEE 480

TDEPTSLSKR KRSIDEILPN DALQDGA 507

Nucleotide Sequence
(FASTA)

ATGGCAGAGG CCCATATCAC ACTATCACCT AAAGTAACTC ATGAACAACA AACAGATATT 60

GATTCTGAAT GTGAAATCAC TGAGGTGGAC GATGAAAATG TAAACGAAAA CAAATCTCAA 120

GAAATGATTC AAGATATCCC TGCACGAGAC CGCGAAGAAA TTGAAAATAT CACAAGGACG 180

TTCGTAGAGC TACAAGAGAA CTATCGGCTA ATTGAAAAAA TTGGAGAAGG TACGTTCTCC 240

AGTGTATATA AAGCGGAAGA CCTACATTAT GGGAGGTATA TTAATGATTG GGACATTCAA 300

AGTGAAGTGT TAAAGGAATC TTCTTTTGGA AAAGAAAAAA TTCCTGTTAA TGAAGACAGC 360

CGGAAACCGA AGTACGTTGC TATAAAAAAA ATTTACGCGA CAAGTAGTCC TGCTAGAATA 420

TACAATGAAT TAGAAATCCT ATATTTATTA AGGGGATCGT CGGTAATAGC CCCGCTAATA 480

ACTGCTTTAA GAAATGAAGA TCAGGTACTC GTAGTTTTAC CCTACTACGA GCATACTGAC 540

TTTCGTCAAT ATTATAGCAC TTTTTCCTAT CGCGATATGA GCATTTATTT TCGATGCCTT 600

TTTCAAGCTA TGCAGCAAAC TCAGACTTTG GGAATCATTC ATAGAGACAT TAAGCCTAGT 660

AACTTTCTCT TCGATGTTCG TACCAAGCAT GGTGTCTTAG TTGACTTTGG ATTAGCAGAG 720

CGTTATGATG GAAGACAACA ATCACATTCT TGCCGGTGCA CTAATTCAAA TGCGGCCGAA 780

TTAGCACATG ATTTTTCGAT TGCGCAGGAA ACATCTCTTG GATACATAAA AAACGATACT 840

CGACCAAGCA AACGGGCCAA TAGAGCCGGA ACTCGAGGAT TTCGAGCACC TGAAGTATTG 900

TTTAAATGCT CATCTCAATC ACCCAAAGTT GATATTTGGT CTGCTGGGGT TATTTTGTTA 960

TCTTTCCTTA CTAAACGTTT CCCCATGTTT AATTCAAAGG ACGACGTGGA TGCACTCATG 1020

GAGATAGCAT GTATATTTGG AAAAAGCGAA ATGCGCCAAT GTGCTGCTTT ACACGGTTGT 1080

ACGTTTGAGA CAAATGTAAG CACGCTTACA GAAAAAAGAG TAAACTTTAG GAAACTTATT 1140

TTGTGGGCCA GTTGTGGTTC AGCCAGTATA TACAAAGAAA AGCTTCGTCA TAAACCAAGC 1200

CAAGAAGAGC GATTGTGCCT GGATTTTTTA GAGAAATGTC TTGAACTTGA CTGTAACAAA 1260

AGGATTTCAG CTGAGGAAGC ATTGGATCAC GATTTCTTGT ATCTCGATAA TTTAGCTTAC 1320

GAAAAGAAAG ATGACGATAC AGCTTTTGAT AATAGCTTTG GAGAAACCTC TTTCGAGAAA 1380

GACGAAGACT TAACTGCTAA GCACTTATCC CATATCCTTG ATTTTAAAGA GCAGGAAGAA 1440

ACAGATGAAC CTACTTCTTT ATCAAAGCGG AAGCGAAGTA TTGATGAAAT TTTACCAAAT 1500

GATGCTTTGC AGGATGGAGC TTGA 1524

Domain Profile

S: 1     fkikdkiGeGtfssvyla.............................taqleeglee...  28

         +++ +kiGeGtfssvy+a

Q: 68    YRLIEKIGEGTFSSVYKAedlhygryindwdiqsevlkessfgkekiP--------Vned  119

         67899**********************999998887666665544333........0344

S: 29    .....kialkhliptshPeriaaeLelltvlgGqenvvglkecfrkndsvvivlpylehe  83

              ++a+k++++ts+P ri++eLe+l+ l G++ +++l +++r +d+v++vlpy+eh

Q: 120   srkpkYVAIKKIYATSSPARIYNELEILYLLRGSSVIAPLITALRNEDQVLVVLPYYEHT  179

         555669******************************************************

S: 84    sfldllrslsfqevreYlrnLlvalkrvhkfgivhrDvkpsnfLynrrlkkfaLvDfGLa  143

         +f++ +  +s++++  Y+r L++a+++    gi+hrD+kpsnfL++ r+k+ +LvDfGLa

Q: 180   DFRQYYSTFSYRDMSIYFRCLFQAMQQTQTLGIIHRDIKPSNFLFDVRTKHGVLVDFGLA  239

         ************************************************************

S: 144   q..seaqkeecsrnllsaavekevdtcdcygtdkvcsvclsrrqqvanraGtpGfraPev  201

         +    +q+ +  r + s+a+e   d    + t+       +r  ++anraGt+GfraPev

Q: 240   EryDGRQQSHSCRCTNSNAAELAHDFSIAQETSLGYIKNDTRPSKRANRAGTRGFRAPEV  299

         96556777777788888888888886666667666666778899****************

S: 202   LlkipdqttaiDiwsaGvilLsllsgryPffkasddlealaeivtilGsreiikaakafg  261

         L+k+++q++++DiwsaGvilLs+l++r+P+f+++dd++al+ei+ i+G+ e++++a  +g

Q: 300   LFKCSSQSPKVDIWSAGVILLSFLTKRFPMFNSKDDVDALMEIACIFGKSEMRQCAALHG  359

         ********************************************************8877

S: 262   ksvllskevp.............................dsdsyePdeaydlldklleln  292

          +     +v                              ++ s e   + d+l+k+lel+

Q: 360   CTFET--NVStltekrvnfrklilwascgsasiykeklrHKPSQEERLCLDFLEKCLELD  417

         65433..222234455568888887777777776666553444446779***********

S: 293   Pakritaeealkhpf  307

          +kri+aeeal h f

Q: 418   CNKRISAEEALDHDF  432

         ************987

Domain Sequence
(FASTA)

YRLIEKIGEG TFSSVYKAED LHYGRYINDW DIQSEVLKES SFGKEKIPVN EDSRKPKYVA 60

IKKIYATSSP ARIYNELEIL YLLRGSSVIA PLITALRNED QVLVVLPYYE HTDFRQYYST 120

FSYRDMSIYF RCLFQAMQQT QTLGIIHRDI KPSNFLFDVR TKHGVLVDFG LAERYDGRQQ 180

SHSCRCTNSN AAELAHDFSI AQETSLGYIK NDTRPSKRAN RAGTRGFRAP EVLFKCSSQS 240

PKVDIWSAGV ILLSFLTKRF PMFNSKDDVD ALMEIACIFG KSEMRQCAAL HGCTFETNVS 300

TLTEKRVNFR KLILWASCGS ASIYKEKLRH KPSQEERLCL DFLEKCLELD CNKRISAEEA 360

LDHDF 365

Keyword

ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona savignyi"; ?>Dasypus novemcinctus"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>

Gene Ontology

GO:0031431	; C:Dbf4-dependent protein kinase complex
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0051301	; P:cell division
GO:0051276	; P:chromosome organization
GO:0006270	; P:DNA replication initiation
GO:0000082	; P:G1/S transition of mitotic cell cycle
GO:0007533	; P:mating type switching
GO:0033314	; P:mitotic cell cycle DNA replication checkpoint
GO:0070602	; P:regulation of centromeric sister chromatid cohesion
GO:2000045	; P:regulation of G1/S transition of mitotic cell cycle
GO:0043111	; P:replication fork arrest
GO:0048478	; P:replication fork protection

KEGG

spo:SPBC776.12c

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013