EKS-SCP-00022

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SCP-00022

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	241.5	2.1E-72	10	272	263

Status

Reviewed

Ensembl Protein

SPCC1259.13.1:pep

UniProt Accession

P34208;

Protein Name

Serine/threonine-protein kinase chk1

Protein Synonyms/Alias

Checkpoint kinase 1;

Gene Name

chk1

Gene Synonyms/Alias

chk1; rad27; SPCC1259.13;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
SPCC1259.13	SPCC1259.13.1:pep	SPCC1259.13.1

Organism

Schizosaccharomyces pombe

Functional Description

Serine/threonine-protein kinase which is required forcheckpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Binds to and phosphorylates CDC25. This leads to negative regulation of CDC25 and prevents mitotic entry.

Protein Length

496

Protein Sequence
(FASTA)

MAQKLDNFPY HIGREIGTGA FASVRLCYDD NAKIYAVKFV NKKHATSCMN AGVWARRMAS 60

EIQLHKLCNG HKNIIHFYNT AENPQWRWVV LEFAQGGDLF DKIEPDVGID EDVAQFYFAQ 120

LMEGISFMHS KGVAHRDLKP ENILLDYNGN LKISDFGFAS LFSYKGKSRL LNSPVGSPPY 180

AAPEITQQYD GSKVDVWSCG IILFALLLGN TPWDEAISNT GDYLLYKKQC ERPSYHPWNL 240

LSPGAYSIIT GMLRSDPFKR YSVKHVVQHP WLTSSTPFRT KNGNCADPVA LASRLMLKLR 300

IDLDKPRLAS SRASQNDSGF SMTQPAFKKN DQKELDRVEV YGALSQPVQL NKNIDVTEIL 360

EKDPSLSQFC ENEGFIKRLA KKAKNFYEIC PPERLTRFYS RASRETIIDH LYDSLRLLAI 420

SVTMKYVRNQ TILYVNLHDK RKCLLQGVIE LTNLGHNLEL INFIKRNGDP LEWRKFFKNV 480

VSSIGKPIVL TDVSQN 496

Nucleotide Sequence
(FASTA)

ATGGCTCAAA AATTAGATAA CTTTCCTTAC CATATTGGTA GAGAAATAGG TACTGGGGCT 60

TTTGCTTCCG TCCGTTTATG TTACGATGAT AATGCTAAAA TATATGCTGT CAAATTTGTC 120

AATAAAAAAC ATGCTACTAG TTGTATGAAT GCAGGGGTAT GGGCTAGAAG GATGGCTAGT 180

GAAATACAAC TTCACAAACT ATGCAATGGA CATAAAAATA TCATTCATTT TTATAATACA 240

GCAGAAAATC CCCAATGGAG ATGGGTAGTA CTTGAATTTG CTCAAGGTGG TGACTTATTT 300

GACAAAATAG AGCCTGATGT AGGTATTGAT GAGGACGTCG CTCAGTTTTA TTTTGCTCAA 360

CTTATGGAAG GTATTTCCTT CATGCACTCT AAAGGTGTCG CGCATCGAGA CTTGAAACCT 420

GAGAACATCC TTTTAGATTA CAATGGTAAC TTAAAGATTT CAGACTTCGG CTTTGCATCT 480

TTATTTTCCT ATAAAGGTAA AAGTAGGCTT TTGAATAGTC CAGTGGGTAG TCCACCATAC 540

GCTGCTCCAG AAATTACACA GCAGTATGAT GGTTCAAAAG TTGATGTCTG GTCCTGCGGT 600

ATAATTCTTT TTGCTTTGTT ATTAGGCAAC ACACCTTGGG ATGAAGCAAT TAGCAACACT 660

GGTGACTACT TGCTTTATAA AAAACAATGT GAACGCCCGT CTTACCACCC ATGGAACTTG 720

TTGTCTCCAG GAGCCTATTC GATTATCACC GGAATGCTTC GAAGTGATCC ATTTAAGCGC 780

TACTCTGTCA AACATGTTGT ACAGCACCCA TGGCTTACCT CAAGTACACC ATTTCGAACT 840

AAAAATGGGA ATTGTGCCGA TCCTGTAGCA TTGGCATCAA GGCTCATGTT GAAACTTCGT 900

ATTGATTTAG ATAAACCACG TCTTGCCTCA TCTAGAGCAT CTCAAAACGA TAGCGGTTTT 960

TCAATGACAC AGCCAGCTTT TAAAAAAAAT GACCAAAAGG AGTTGGATCG TGTAGAAGTT 1020

TATGGCGCCT TATCCCAGCC AGTACAACTT AATAAAAATA TTGACGTTAC TGAAATCCTT 1080

GAAAAAGACC CCTCATTGAG TCAGTTTTGT GAGAATGAAG GTTTTATTAA AAGATTGGCC 1140

AAAAAGGCTA AGAATTTCTA CGAAATTTGT CCTCCTGAAA GACTCACTAG GTTTTATTCT 1200

AGGGCGTCGA GAGAAACCAT TATAGATCAT CTTTATGACA GTCTACGACT ACTTGCAATC 1260

TCAGTGACTA TGAAATATGT TCGAAATCAG ACCATCCTTT ATGTTAATTT ACATGATAAA 1320

CGGAAATGTC TCTTGCAAGG TGTTATTGAA CTAACTAATT TAGGCCATAA CTTAGAGCTT 1380

ATAAACTTTA TAAAGCGTAA TGGGGATCCT CTTGAATGGA GAAAATTTTT TAAAAACGTT 1440

GTCAGTTCAA TAGGGAAGCC GATTGTTCTT ACAGATGTTT CACAAAATTA A 1491

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl.....seeslekikrEieilkkl  55

         y++++++G+g+f+ V+l+ + +  +  avK+++kk++        +++++ +Ei+++k +

Q: 10    YHIGREIGTGAFASVRLCYDDN-AKIYAVKFVNKKHAtscmnAGVWARRMASEIQLHKLC  68

         899***************9875.5789*******998666643344789***********

S: 56    k.hpniikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYl  114

         + h+nii++++  e+ +  ++vle+a+gg+Lfd+ie +  ++e+ a+ +f+ql+++++++

Q: 69    NgHKNIIHFYNTAENPQWRWVVLEFAQGGDLFDKIEPDVGIDEDVAQFYFAQLMEGISFM  128

         77**********************************************************

S: 115   hskgivHrDlKpeNllldkkenikiaDFGlanlfkeg...klletfcGsppYaaPellkg  171

         hskg++HrDlKpeN+lld ++n+ki+DFG+a+lf+++   +ll++ +GsppYaaPe+++

Q: 129   HSKGVAHRDLKPENILLDYNGNLKISDFGFASLFSYKgksRLLNSPVGSPPYAAPEITQ-  187

         ********************************9998878899***************87.

S: 172   kkYegpkvDvWslGvvLyalvtgklPfdeenlkellekilkgkleipkk.....lskele  226

          +Y+g+kvDvWs+G++L+al+ g++P+de+  ++    + k+++e p++     ls+ +

Q: 188   -QYDGSKVDVWSCGIILFALLLGNTPWDEAISNTGDYLLYKKQCERPSYhpwnlLSPGAY  246

         .9***************************9444433333444444444434446******

S: 227   sLlrklLvvdpekRatieeilkhewl  252

         s+++ +L  dp kR++++++++h+wl

Q: 247   SIITGMLRSDPFKRYSVKHVVQHPWL  272

         *************************7

Domain Sequence
(FASTA)

YHIGREIGTG AFASVRLCYD DNAKIYAVKF VNKKHATSCM NAGVWARRMA SEIQLHKLCN 60

GHKNIIHFYN TAENPQWRWV VLEFAQGGDL FDKIEPDVGI DEDVAQFYFA QLMEGISFMH 120

SKGVAHRDLK PENILLDYNG NLKISDFGFA SLFSYKGKSR LLNSPVGSPP YAAPEITQQY 180

DGSKVDVWSC GIILFALLLG NTPWDEAISN TGDYLLYKKQ CERPSYHPWN LLSPGAYSII 240

TGMLRSDPFK RYSVKHVVQH PWL 263

Keyword

ATP-binding; Cell cycle; Complete proteome; DNA damage; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>

Gene Ontology

GO:0005829	; C:cytosol
GO:0005654	; C:nucleoplasm
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007049	; P:cell cycle
GO:0000077	; P:DNA damage checkpoint

KEGG

spo:SPCC1259.13

;

InterPros

IPR020636	; Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013