EKS-CAE-00217

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00217

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	340.9	6.2E-102	25	277	253

Status

Reviewed

Ensembl Protein

T01C8.1c.1

UniProt Accession

Q95ZQ4; Q22068; Q86FL6;

Protein Name

5'-AMP-activated protein kinase catalytic subunit alpha-2

Protein Synonyms/Alias

AMPK subunit alpha-2;

Gene Name

aak-2

Gene Synonyms/Alias

aak-2; T01C8.1;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
T01C8.1	T01C8.1c.1	T01C8.1c.1
T01C8.1	T01C8.1b	T01C8.1b
T01C8.1	T01C8.1a	T01C8.1a
T01C8.1	T01C8.1c.2	T01C8.1c.2

Organism

Caenorhabditis elegans

Functional Description

Acts as a sensor that couples lifespan to informationabout energy levels and insulin-like signals. Role in motility and response to oxidative stress. Causes germ cell cycle arrest upon dauer entry.

Protein Length

562

Protein Sequence
(FASTA)

MSSPGGETST KQQQELKAQI KIGHYILKET LGVGTFGKVK VGIHETTQYK VAVKILNRQK 60

IKSLDVVGKI RREIQNLSLF RHPHIIRLYQ VISTPSDIFM IMEHVSGGEL FDYIVKHGRL 120

KTAEARRFFQ QIISGVDYCH RHMVVHRDLK PENLLLDEQN NVKIADFGLS NIMTDGDFLR 180

TSCGSPNYAA PEVISGKLYA GPEVDVWSCG VILYALLCGT LPFDDEHVPS LFRKIKSGVF 240

PTPDFLERPI VNLLHHMLCV DPMKRATIKD VIAHEWFQKD LPNYLFPPIN ESEASIVDIE 300

AVREVTERYH VAEEEVTSAL LGDDPHHHLS IAYNLIVDNK RIADETAKLS IEEFYQVTPN 360

KGPGPVHRHP ERIAASVSSK ITPTLDNTEA SGANRNKRAK WHLGIRSQSR PEDIMFEVFR 420

AMKQLDMEWK VLNPYHVIVR RKPDAPAADP PKMSLQLYQV DQRSYLLDFK SLADEESGSA 480

SASSSRHASM SMPQKPAGIR GTRTSSMPQA MSMEASIEKM EVHDFSDMSC DVTPPPSPGG 540

AKLSQTMQFF EICAALIGTL AR 562

Nucleotide Sequence
(FASTA)

ATGTCGTCTC CAGGAGGAGA AACGTCGACG AAGCAGCAAC AAGAGCTCAA AGCTCAAATC 60

AAAATCGGAC ATTACATTCT CAAGGAGACA CTCGGAGTTG GTACTTTTGG TAAAGTAAAA 120

GTTGGAATCC ATGAGACAAC TCAATACAAA GTGGCTGTCA AGATTCTGAA CCGTCAGAAG 180

ATCAAGTCAC TGGATGTCGT TGGAAAGATT CGCCGCGAAA TCCAAAACCT CTCGCTCTTC 240

CGCCATCCGC ATATCATCCG CCTCTACCAA GTCATCAGTA CACCTTCTGA CATTTTCATG 300

ATTATGGAGC ACGTTTCCGG CGGGGAGCTC TTTGACTACA TTGTTAAGCA CGGACGGCTG 360

AAGACCGCAG AAGCTCGTCG CTTCTTTCAA CAAATCATTT CCGGCGTTGA CTACTGTCAT 420

CGTCATATGG TTGTCCATAG AGATTTGAAG CCAGAGAATT TGTTGCTCGA TGAGCAGAAC 480

AATGTGAAGA TTGCGGACTT TGGACTTTCA AATATTATGA CGGATGGTGA CTTCTTACGC 540

ACCAGCTGCG GATCGCCAAA TTATGCTGCC CCTGAGGTTA TTAGCGGAAA GTTGTACGCA 600

GGTCCCGAAG TTGATGTATG GTCGTGTGGA GTCATTTTGT ATGCACTTCT TTGTGGAACC 660

CTGCCATTTG ATGATGAGCA CGTGCCAAGT CTTTTCAGAA AAATTAAATC TGGCGTATTC 720

CCAACTCCAG ACTTTCTGGA GCGCCCAATT GTAAATCTGC TTCACCATAT GCTCTGCGTA 780

GACCCGATGA AGAGGGCCAC CATCAAGGAC GTCATTGCTC ACGAGTGGTT CCAGAAGGAT 840

TTGCCGAACT ACTTGTTCCC ACCAATCAAC GAGAGTGAGG CTTCCATTGT TGATATTGAG 900

GCTGTCCGAG AGGTCACTGA GCGCTATCAT GTCGCCGAAG AGGAAGTCAC CTCAGCATTG 960

CTGGGAGATG ATCCACATCA CCATTTGTCG ATTGCATATA ACTTGATTGT TGATAACAAG 1020

AGAATTGCCG ATGAGACTGC CAAGTTGTCA ATTGAGGAGT TTTATCAAGT GACGCCGAAT 1080

AAGGGACCTG GACCAGTTCA TCGCCATCCA GAGCGCATTG CAGCGTCAGT CAGCAGCAAG 1140

ATCACACCAA CTCTCGACAA CACGGAAGCC AGTGGCGCGA ACCGCAACAA ACGTGCCAAG 1200

TGGCATCTGG GTATCCGATC CCAAAGTCGT CCGGAAGACA TCATGTTCGA GGTGTTCCGT 1260

GCGATGAAGC AGCTGGACAT GGAGTGGAAG GTGCTCAACC CATATCATGT GATTGTTCGT 1320

CGCAAGCCTG ATGCACCCGC TGCCGACCCG CCAAAGATGT CGTTGCAGTT GTACCAAGTG 1380

GATCAGAGAA GTTACTTGTT GGATTTCAAG AGTTTGGCCG ACGAGGAGTC TGGATCCGCC 1440

AGTGCATCTT CATCCCGACA CGCATCAATG TCTATGCCGC AGAAGCCGGC CGGAATTCGT 1500

GGAACTAGAA CGTCAAGCAT GCCACAGGCG ATGAGTATGG AGGCGAGTAT TGAGAAAATG 1560

GAAGTGCATG ATTTTTCGGA CATGTCGTGC GATGTGACAC CACCACCATC TCCTGGAGGA 1620

GCTAAGCTTT CCCAGACTAT GCAATTCTTT GAGATTTGCG CCGCATTGAT TGGAACACTG 1680

GCTCGTTAA 1689

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl.seeslekikrEieilkklkhpn  59

         y l +tlG g+fgkVk+ +h++t+ kvavKi++++k+ s + + ki+rEi+ l+ ++hp+

Q: 25    YILKETLGVGTFGKVKVGIHETTQYKVAVKILNRQKIkSLDVVGKIRREIQNLSLFRHPH  84

         7899********************************95556778****************

S: 60    iikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlhskgi  119

         ii+l++vi+t +++++++e++sggeLfdyi+++grlk  ear++f+q++s+v+Y+h++ +

Q: 85    IIRLYQVISTPSDIFMIMEHVSGGELFDYIVKHGRLKTAEARRFFQQIISGVDYCHRHMV  144

         ************************************************************

S: 120   vHrDlKpeNllldkkenikiaDFGlanlfkegklletfcGsppYaaPellkgkkYegpkv  179

         vHrDlKpeNllld+++n+kiaDFGl+n++++g+ l+t+cGsp+YaaPe+++gk Y gp+v

Q: 145   VHRDLKPENLLLDEQNNVKIADFGLSNIMTDGDFLRTSCGSPNYAAPEVISGKLYAGPEV  204

         ************************************************************

S: 180   DvWslGvvLyalvtgklPfdeenlkellekilkgkleipkklskelesLlrklLvvdpek  239

         DvWs+Gv+Lyal++g+lPfd+e++ +l++ki++g + +p+ l++ + +Ll+++L+vdp k

Q: 205   DVWSCGVILYALLCGTLPFDDEHVPSLFRKIKSGVFPTPDFLERPIVNLLHHMLCVDPMK  264

         ************************************************************

S: 240   Ratieeilkhewl  252

         Rati+++ +hew+

Q: 265   RATIKDVIAHEWF  277

         ************7

Domain Sequence
(FASTA)

YILKETLGVG TFGKVKVGIH ETTQYKVAVK ILNRQKIKSL DVVGKIRREI QNLSLFRHPH 60

IIRLYQVIST PSDIFMIMEH VSGGELFDYI VKHGRLKTAE ARRFFQQIIS GVDYCHRHMV 120

VHRDLKPENL LLDEQNNVKI ADFGLSNIMT DGDFLRTSCG SPNYAAPEVI SGKLYAGPEV 180

DVWSCGVILY ALLCGTLPFD DEHVPSLFRK IKSGVFPTPD FLERPIVNLL HHMLCVDPMK 240

RATIKDVIAH EWF 253

Keyword

Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Stress response; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Saccharomyces cerevisiae"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0030424	; C:axon
GO:0005737	; C:cytoplasm
GO:0043025	; C:neuronal cell body
GO:0004679	; F:AMP-activated protein kinase activity
GO:0005524	; F:ATP binding
GO:0008340	; P:determination of adult lifespan
GO:0051729	; P:germline cell cycle switching, mitotic to meiotic cell cycle
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0061066	; P:positive regulation of dauer larval development
GO:0006950	; P:response to stress

KEGG

cel:CELE_T01C8.1

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013