EPS-CAE-00013

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-CAE-00013

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPM/PP2C	156.3	1.9E-45	168	420	253

Status

Reviewed

Ensembl Protein

T19C3.8

UniProt Accession

P49594;

Protein Name

Ca(2+)/calmodulin-dependent protein kinase phosphatase

Protein Synonyms/Alias

CaM-kinase phosphatase; CaMKPase; Feminization of XX and XO animals protein 2; Sex-determining protein fem-2;

Gene Name

fem-2

Gene Synonyms/Alias

fem-2; T19C3.8;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
T19C3.8	T19C3.8	T19C3.8

Organism

Caenorhabditis elegans

Functional Description

Probable phosphatase involved in the regulation of sexdetermination. Plays an important role in regulating a pathway transducing a non-cell-autonomous signal to a nuclear transcription factor. Promotes apoptosis. Together with fem-3 associates with the CBC(fem-1) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of tra-1.

Protein Length

449

Protein Sequence
(FASTA)

MEKVNEERDA VFEDHIGDRR RSVRSLLEEA FADEMEKTSY DVEVADTPQP HIPIRFRHPP 60

IAGPVHDVFG DAIHDIFQKM MKRGQAVDFC HWVSHLIATE IDEKFSEVAF RDVQYNPDIY 120

VTDSTTEAKK LFNDKIWPAI DKILQQNAET CPILSEKWSG IHVSGDQLKG QRHKQEDRFL 180

AYPNGQYMDR GEDPISVLAV FDGHGGHECS QYAAGHLWET WLEVRKSRDP SDSLEDQLRK 240

SLELLDERMT VRSVKECWKG GSTAVCCAID MDQKLMALAW LGDSPGYVMS NIEFRQLTRG 300

HSPSDEREAR RVEEAGGQLF VIGGELRVNG VLNLTRALGD VPGRPMISNE PETCQVPIES 360

SDYLVLLACD GISDVFNERD LYQLVEAFAN DYPVEDYAEL SRFICTKAIE AGSADNVSVV 420

IGFLRPPQDV WKLMKHESDD EDSDVTDEE 449

Nucleotide Sequence
(FASTA)

ATGGAAAAAG TAAACGAGGA GCGGGATGCG GTTTTCGAGG ATCACATCGG CGATCGCCGT 60

CGAAGTGTCC GGTCACTTCT GGAGGAGGCA TTTGCAGATG AAATGGAGAA AACTAGCTAT 120

GACGTCGAGG TTGCCGACAC TCCACAACCG CATATTCCAA TACGTTTCCG TCATCCACCA 180

ATCGCCGGAC CAGTTCATGA TGTTTTCGGA GACGCGATTC ACGACATTTT CCAGAAAATG 240

ATGAAAAGAG GCCAGGCGGT CGACTTTTGT CACTGGGTGT CTCACTTGAT CGCCACAGAA 300

ATTGACGAGA AATTCAGTGA AGTTGCGTTC AGAGATGTTC AGTATAATCC TGATATTTAT 360

GTTACCGATA GTACCACAGA AGCCAAAAAG CTATTCAACG ACAAAATTTG GCCGGCGATC 420

GACAAGATTC TCCAGCAAAA CGCCGAAACG TGCCCGATTC TGTCTGAAAA GTGGTCTGGA 480

ATCCACGTGT CGGGCGATCA ACTGAAAGGG CAACGTCACA AGCAAGAAGA TCGGTTTTTG 540

GCGTATCCTA ATGGGCAATA TATGGATCGT GGAGAGGATC CAATTTCGGT GTTAGCGGTG 600

TTCGATGGTC ACGGTGGTCA CGAGTGCTCT CAGTACGCAG CCGGGCACCT TTGGGAGACA 660

TGGTTGGAGG TTCGAAAATC GCGAGATCCT TCAGATAGCC TCGAAGATCA GCTTCGCAAG 720

TCGCTGGAGC TTCTCGACGA ACGAATGACT GTCAGAAGTG TGAAAGAGTG TTGGAAGGGT 780

GGAAGTACAG CTGTCTGCTG TGCAATCGAC ATGGATCAGA AGCTTATGGC GCTGGCGTGG 840

CTCGGTGACT CACCCGGATA CGTCATGTCG AACATTGAAT TCCGTCAGTT GACACGTGGA 900

CACTCGCCAT CCGATGAACG GGAAGCTCGG CGTGTTGAGG AAGCTGGCGG CCAGCTTTTT 960

GTGATTGGAG GCGAATTGCG GGTGAACGGA GTTCTAAATC TCACCCGAGC ACTCGGAGAT 1020

GTTCCTGGAC GGCCGATGAT CTCTAATGAA CCGGAAACGT GTCAGGTGCC TATTGAAAGC 1080

TCGGATTATC TGGTGCTCCT GGCTTGTGAT GGAATTTCTG ATGTTTTCAA CGAGCGTGAC 1140

CTGTACCAGT TGGTGGAGGC ATTTGCGAAT GATTATCCTG TTGAAGACTA CGCGGAGCTC 1200

TCCCGTTTCA TCTGTACAAA AGCGATTGAG GCTGGAAGTG CCGACAACGT GTCTGTTGTG 1260

ATTGGCTTTT TGCGTCCACC GCAGGATGTG TGGAAGCTTA TGAAACATGA ATCAGACGAT 1320

GAAGATTCCG ATGTCACTGA TGAGGAATAA 1350

Domain Profile

S: 8     vngrrnemedavtsspnflkspekkdrlaffgvyDGhggsevakfcseklheilaeeles  67

         ++g+r++ ed++ ++pn       +d +++ +v+DGhgg+e +++++ +l+e + e  +

Q: 168   LKGQRHKQEDRFLAYPNGQYMDRGEDPISVLAVFDGHGGHECSQYAAGHLWETWLEVRK-  226

         789**************9999999999***************************99866.

S: 68    seseerkvkkalakaFlstdeelkvleeksldidksGstavvalikds..klyvanvGdS  125

         s + ++ +++ l+k+++ +de++  +++  +++ k Gstav++ i+ +   +  a +GdS

Q: 227   SRDPSDSLEDQLRKSLELLDERM--TVRSVKECWKGGSTAVCCAIDMDqkLMALAWLGDS  284

         8999999****************..666668**************9776345559*****

S: 126   ravLcrngkavelssdHkpsredEleRieeaggsvilkdga.RvlGvLavsRalGdlylk  184

             + n +  +l++ H+ps e+E +R+eeagg+     g+ Rv+GvL+++RalGd+

Q: 285   PGYVMSNIEFRQLTRGHSPSDEREARRVEEAGGQLFVIGGElRVNGVLNLTRALGDVPGR  344

         99999999999*************************************************

S: 185   pyvtpePevtshrltsedefliLasDGlwdvvsneeavslvrdvle..kkeaeqeaakkL  242

         p+++ ePe+ +++++s+d  + La+DG+ dv+++ ++++lv+ + +    e+  e +  +

Q: 345   PMISNEPETCQVPIESSDYLVLLACDGISDVFNERDLYQLVEAFANdyPVEDYAELSRFI  404

         **********************************************5545556666778*

S: 243   vklalargskdnvsai  258

         ++ a++ gs dnvs++

Q: 405   CTKAIEAGSADNVSVV  420

         *************776

Domain Sequence
(FASTA)

LKGQRHKQED RFLAYPNGQY MDRGEDPISV LAVFDGHGGH ECSQYAAGHL WETWLEVRKS 60

RDPSDSLEDQ LRKSLELLDE RMTVRSVKEC WKGGSTAVCC AIDMDQKLMA LAWLGDSPGY 120

VMSNIEFRQL TRGHSPSDER EARRVEEAGG QLFVIGGELR VNGVLNLTRA LGDVPGRPMI 180

SNEPETCQVP IESSDYLVLL ACDGISDVFN ERDLYQLVEA FANDYPVEDY AELSRFICTK 240

AIEAGSADNV SVV 253

Keyword

Apoptosis; Complete proteome; Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase; Reference proteome; Ubl conjugation pathway.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0033192	; F:calmodulin-dependent protein phosphatase activity
GO:0046872	; F:metal ion binding
GO:0042006	; P:masculinization of hermaphroditic germ-line
GO:0035970	; P:peptidyl-threonine dephosphorylation
GO:0043280	; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process

KEGG

cel:CELE_T19C3.8

;

InterPros

IPR001932	; PP2C-like.
IPR000222	; PP2C_Mn2_Asp60_BS.
IPR015655	; Protein_Pase_2C.

Pfam

PF00481

; PP2C; 1.

SMARTs

SM00331	; PP2C_SIG; 1.
SM00332	; PP2Cc; 1.

Prosites

PS01032

; PP2C; 1.

Prints

Created Date

20-Feb-2013