EKS-CAE-00230

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-CAE-00230

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	228.2	1.5E-67	42	305	264

Status

Reviewed

Ensembl Protein

Y59A8B.14b

UniProt Accession

Q9GN62; G5EES7; G5EGA8; Q9Y0C1;

Protein Name

Serine/threonine-protein kinase par-4

Protein Synonyms/Alias

Gene Name

par-4

Gene Synonyms/Alias

par-4; Y59A8B.14;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
Y59A8B.14	Y59A8B.14c	Y59A8B.14c
Y59A8B.14	Y59A8B.14a	Y59A8B.14a
Y59A8B.14	Y59A8B.14b	Y59A8B.14b

Organism

Caenorhabditis elegans

Functional Description

Required for cytoplasmic partitioning and asymmetriccell division in early embryogenesis. Involved in mediating cell polarization via regulation of anillin family scaffold proteins. Phosphorylates and restricts the asymmetry effectors mex-5 and mex-6 to the anterior cytoplasm of the zygote and maintains these phosphorylations until fertilization. Phosphorylates and regulates aak-2 in response to oxidative stress. May also play a role in motility, behavioral response, regulation of lifespan and dauer formation through this pathway. Acts downstream of unc-40 in dendrite outgrowth.

Protein Length

476

Protein Sequence
(FASTA)

MRQFINQQVN NAMMFNQDNS EFQHIEFEPI VKQKGPKIIE GYMWGGQIGT GSYGKVKECI 60

DMYTLTRRAV KIMKYDKLRK ITNGWENIRS EMSILRRMNH RNVIKLIEIF NIPAKGKVYM 120

VFEYCIGSVQ QLLDMEPARR LTIGESHAIF IELCQGLNYL HSKRVSHKDI KPGNLLVSID 180

FTVKICDFGV AEQINLFQRD GRCTKVNGTP KFQPPECIYG NHDFFDGYKA DMWSAGVTLY 240

NLVSGKYPFE KPVLLKLYEC IGTEPLQMPT NVQLTKDLQD LLTKLLEKDF NERPTCLETM 300

IHPWFLSTFP EDQGLGRIME RMRTGDRPLT MLSSMTALYD GITPEDELII EDNLGIIQQI 360

LPINLTSEAV LERGSFPGFK FLEAKPGDGP DGVEGSEDSA APLGPQRRPS SRSMPTCAPP 420

GPAAGNAQNS TAENGAETDG VASASDPPPT AAPGAPPRRR KRNFFSCIFR SRTDSA 476

Nucleotide Sequence
(FASTA)

ATGCGTCAAT TTATCAATCA ACAAGTGAAT AATGCAATGA TGTTTAATCA GGATAATTCG 60

GAATTTCAGC ACATTGAATT CGAGCCAATT GTGAAGCAAA AAGGACCGAA AATAATTGAA 120

GGATACATGT GGGGAGGACA AATTGGTACA GGATCATATG GAAAAGTGAA AGAATGTATT 180

GATATGTATA CACTTACACG ACGGGCCGTC AAAATTATGA AATATGATAA ATTACGGAAA 240

ATTACGAATG GATGGGAGAA TATTCGAAGT GAAATGTCGA TTTTAAGGCG GATGAATCAT 300

AGGAATGTTA TTAAATTGAT TGAGATCTTC AATATTCCGG CTAAGGGAAA AGTCTACATG 360

GTATTCGAGT ACTGTATTGG TTCAGTTCAA CAACTTCTCG ACATGGAACC GGCTCGACGT 420

CTAACCATCG GAGAATCTCA TGCAATTTTT ATTGAACTCT GTCAGGGACT CAATTATTTA 480

CACTCGAAAC GTGTATCGCA TAAGGACATT AAACCCGGCA ATTTGCTCGT TTCTATCGAC 540

TTTACCGTGA AAATTTGCGA TTTTGGAGTT GCAGAGCAGA TTAACTTGTT TCAGAGAGAC 600

GGACGATGCA CAAAAGTCAA CGGGACCCCG AAATTTCAGC CGCCTGAATG TATTTATGGA 660

AATCATGATT TTTTTGATGG CTACAAAGCG GATATGTGGA GTGCAGGTGT CACCCTGTAC 720

AACCTGGTTT CTGGAAAATA TCCATTCGAA AAGCCTGTTC TATTGAAATT GTATGAATGT 780

ATTGGAACTG AGCCACTACA AATGCCAACT AATGTTCAAT TGACTAAGGA TCTACAGGAT 840

TTGCTCACAA AACTTCTGGA AAAAGACTTT AACGAGCGTC CAACGTGTCT CGAAACAATG 900

ATTCATCCAT GGTTTCTGTC AACTTTCCCA GAGGATCAAG GCCTTGGCCG TATAATGGAG 960

CGAATGAGAA CAGGAGATCG ACCATTAACA ATGCTTTCAA GTATGACAGC ATTATATGAT 1020

GGAATAACAC CTGAAGATGA ATTAATTATT GAAGATAATC TTGGAATTAT ACAACAAATT 1080

TTACCCATTA ATTTGACTTC TGAAGCAGTC CTCGAACGTG GCTCATTTCC CGGCTTCAAA 1140

TTTCTCGAAG CAAAACCCGG AGATGGTCCT GACGGTGTCG AAGGATCCGA AGATTCGGCA 1200

GCACCTCTGG GACCTCAACG ACGTCCCTCA TCCCGATCAA TGCCAACGTG TGCACCGCCC 1260

GGTCCAGCAG CTGGAAATGC TCAAAATTCG ACTGCTGAAA ATGGTGCTGA AACTGATGGT 1320

GTTGCTTCAG CTTCAGATCC TCCTCCTACT GCTGCTCCTG GAGCTCCACC GCGACGCCGA 1380

AAACGCAACT TTTTCTCGTG TATCTTTCGT TCTCGTACCG ATAGTGCTTA G 1431

Domain Profile

S: 1     ykllktlGegsfgkVklakhketkekvavKiikkkkl..seeslekikrEieilkklkhp  58

         y ++ ++G+gs+gkVk++++++t ++ avKi+k +kl   ++ +e+i++E++il++++h+

Q: 42    YMWGGQIGTGSYGKVKECIDMYTLTRRAVKIMKYDKLrkITNGWENIRSEMSILRRMNHR  101

         78899*****************************99954445689***************

S: 59    niikllevi..etkkklylvleyasgg..eLfdyieekgrlkekearklfkqlvsavkYl  114

         n+ikl+e++    k k+y+v+ey+ g   +L+d+ e ++rl+  e++++f +l+++++Yl

Q: 102   NVIKLIEIFniPAKGKVYMVFEYCIGSvqQLLDM-EPARRLTIGESHAIFIELCQGLNYL  160

         ********85568999********9877689987.9************************

S: 115   hskgivHrDlKpeNllldkkenikiaDFGlan...lfkegklletfcGsppYaaPellkg  171

         hsk++ H+D+Kp Nll++ + ++ki+DFG+a+   lf+++  ++++ G+p++++Pe++ g

Q: 161   HSKRVSHKDIKPGNLLVSIDFTVKICDFGVAEqinLFQRDGRCTKVNGTPKFQPPECIYG  220

         *******************************966678999*****************988

S: 172   ..kkYegpkvDvWslGvvLyalvtgklPfdeenlkellekilkgkleipkk..lskeles  227

           + ++g k+D+Ws+Gv+Ly+lv+gk+Pf+++ l +l+e i ++ l++p++  l+k+l++

Q: 221   nhDFFDGYKADMWSAGVTLYNLVSGKYPFEKPVLLKLYECIGTEPLQMPTNvqLTKDLQD  280

         3356*********************************************998899*****

S: 228   LlrklLvvdpekRatieeilkhewl  252

         Ll+klL++d ++R+t+ e + h+w+

Q: 281   LLTKLLEKDFNERPTCLETMIHPWF  305

         ************************7

Domain Sequence
(FASTA)

YMWGGQIGTG SYGKVKECID MYTLTRRAVK IMKYDKLRKI TNGWENIRSE MSILRRMNHR 60

NVIKLIEIFN IPAKGKVYMV FEYCIGSVQQ LLDMEPARRL TIGESHAIFI ELCQGLNYLH 120

SKRVSHKDIK PGNLLVSIDF TVKICDFGVA EQINLFQRDG RCTKVNGTPK FQPPECIYGN 180

HDFFDGYKAD MWSAGVTLYN LVSGKYPFEK PVLLKLYECI GTEPLQMPTN VQLTKDLQDL 240

LTKLLEKDFN ERPTCLETMI HPWF 264

Keyword

Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Developmental protein; Kinase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Stress response; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005938	; C:cell cortex
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0008356	; P:asymmetric cell division
GO:0045167	; P:asymmetric protein localization involved in cell fate determination
GO:0008340	; P:determination of adult lifespan
GO:0030010	; P:establishment of cell polarity
GO:0018107	; P:peptidyl-threonine phosphorylation
GO:0061066	; P:positive regulation of dauer larval development
GO:0040017	; P:positive regulation of locomotion
GO:0031991	; P:regulation of actomyosin contractile ring contraction
GO:0006979	; P:response to oxidative stress

KEGG

cel:CELE_Y59A8B.14

;

InterPros

IPR020636	; Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013