| Tag | Content |
|---|
| EKPD ID | EKS-SAC-00101 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| CMGC/MAPK | 399.0 | 3.5E-120 | 14 | 309 | 296 |
|
| Status | Reviewed |
| Ensembl Protein | YBL016W |
| UniProt Accession | P16892; D6VPY4; |
| Protein Name | Mitogen-activated protein kinase FUS3 |
| Protein Synonyms/Alias | MAP kinase FUS3; |
| Gene Name | FUS3 |
| Gene Synonyms/Alias | FUS3; DAC2; YBL016W; YBL03.21, YBL0303; |
| Ensembl Information | |
| Organism | Saccharomyces cerevisiae |
| Functional Description | Together with closely related KSS1, FUS3 is the finalkinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated FUS3 activates the mating but suppresses the filamentation pathway, whereas activated KSS1 activates both pathways. Pheromone-activated FUS3 functions by inhibiting the binding of the transcriptional activator STE12 to filamentation specific genes while inducing its binding to and activity at mating specific genes. Non-activated FUS3 has a repressive effect on STE12 transcriptional activity. KSS1 can partially compensate for the lack of FUS3 but mating efficiency is reduced and the filamentation program is partially activated upon pheromone signaling. FUS3 phosphorylates STE7, STE5, FAR1, DIG1, DIG2 and STE12. |
| Protein Length | 353 |
Protein Sequence
(FASTA) | | MPKRIVYNIS SDFQLKSLLG EGAYGVVCSA THKPTGEIVA IKKIEPFDKP LFALRTLREI 60 | | KILKHFKHEN IITIFNIQRP DSFENFNEVY IIQELMQTDL HRVISTQMLS DDHIQYFIYQ 120 | | TLRAVKVLHG SNVIHRDLKP SNLLINSNCD LKVCDFGLAR IIDESAADNS EPTGQQSGMT 180 | | EYVATRWYRA PEVMLTSAKY SRAMDVWSCG CILAELFLRR PIFPGRDYRH QLLLIFGIIG 240 | | TPHSDNDLRC IESPRAREYI KSLPMYPAAP LEKMFPRVNP KGIDLLQRML VFDPAKRITA 300 | | KEALEHPYLQ TYHDPNDEPE GEPIPPSFFE FDHYKEALTT KDLKKLIWNE IFS 353 |
|
Nucleotide Sequence
(FASTA) | | ATGCCAAAGA GAATTGTATA CAATATATCC AGTGACTTCC AGTTGAAGTC GTTACTGGGA 60 | | GAGGGTGCAT ACGGTGTGGT ATGTTCTGCA ACGCATAAGC CCACGGGAGA AATCGTGGCA 120 | | ATAAAAAAGA TCGAACCATT CGATAAGCCT TTGTTCGCAT TACGTACGCT GCGTGAAATA 180 | | AAGATCCTGA AGCACTTCAA GCACGAAAAT ATCATAACAA TCTTCAACAT TCAACGCCCT 240 | | GACTCGTTCG AAAACTTCAA TGAGGTCTAC ATAATTCAAG AGCTAATGCA GACAGATTTA 300 | | CACCGTGTAA TCTCCACCCA GATGCTGAGT GACGATCATA TACAATATTT TATATACCAA 360 | | ACCTTGAGAG CAGTGAAAGT GCTGCATGGT TCGAACGTCA TCCATCGTGA TTTAAAGCCC 420 | | TCCAACCTTC TCATAAACTC CAACTGTGAC TTGAAAGTAT GTGATTTCGG TTTAGCAAGA 480 | | ATCATTGACG AGTCAGCCGC GGACAATTCA GAGCCCACAG GTCAGCAAAG CGGCATGACC 540 | | GAGTATGTGG CCACACGTTG GTACAGGGCG CCAGAGGTGA TGTTAACCTC TGCCAAATAC 600 | | TCAAGGGCCA TGGACGTGTG GTCCTGCGGA TGTATTCTCG CTGAACTTTT CTTAAGACGG 660 | | CCAATCTTCC CTGGCAGAGA TTATCGCCAT CAACTACTAC TGATATTCGG TATCATCGGT 720 | | ACACCTCACT CAGATAATGA TTTGCGGTGT ATAGAGTCAC CCAGGGCTAG AGAGTACATA 780 | | AAGTCGCTTC CCATGTACCC TGCCGCGCCA CTGGAGAAGA TGTTCCCTCG AGTCAACCCG 840 | | AAAGGCATAG ATCTTTTACA GCGTATGCTT GTTTTTGACC CTGCGAAGAG GATTACTGCT 900 | | AAGGAGGCAC TGGAGCATCC GTATTTGCAA ACATACCACG ATCCAAACGA CGAACCTGAA 960 | | GGCGAACCCA TCCCACCCAG CTTCTTCGAG TTTGATCACT ACAAGGAGGC ACTAACGACG 1020 | | AAAGACCTCA AGAAACTCAT TTGGAACGAA ATATTTAGTT AG 1062 |
|
| Domain Profile | S: 2 eslkplgeGaygvvvsavdkrteervaikklsrpfqketsakrtlRElkllkelkheNii 61 | + ++ lgeGaygvv+sa++k t+e vaikk+ pf+k a rtlRE+k+lk++kheNii | Q: 14 QLKSLLGEGAYGVVCSATHKPTGEIVAIKKIE-PFDKPLFALRTLREIKILKHFKHENII 72 | 667889*************************9.*************************** |
| S: 62 klldvftpeeeleelkdvYlvtelmetdLkkviksqklsdehiklllyqilrglkylHsa 121 | +++++ +p++ +e++++vY+++elm+tdL++vi++q lsd+hi++++yq lr++k lH + | Q: 73 TIFNIQRPDS-FENFNEVYIIQELMQTDLHRVISTQMLSDDHIQYFIYQTLRAVKVLHGS 131 | *********9.************************************************* |
| S: 122 nviHrDlKPsNllvnedcelkildFGlarsadkekekk.........lteyvatrwYraP 172 | nviHrDlKPsNll+n++c+lk++dFGlar+ d++ + +teyvatrwYraP | Q: 132 NVIHRDLKPSNLLINSNCDLKVCDFGLARIIDESAADNseptgqqsgMTEYVATRWYRAP 191 | ******************************88776655678999**************** |
| S: 173 eillslkeytkavDiWsvGCIlaElltgkplfpgkdeidqlekilevlgtp.seeflkki 231 | e++l++ +y++a+D+Ws+GCIlaEl+ ++p+fpg+d+ +ql +i+ ++gtp s+++l++i | Q: 192 EVMLTSAKYSRAMDVWSCGCILAELFLRRPIFPGRDYRHQLLLIFGIIGTPhSDNDLRCI 251 | ***************************************************44689**** |
| S: 232 eseearnyikslpkkkkkdfeelfpkaseealdLleklLvldpdkRisveeaLehpYl 289 | es +ar+yikslp+++++++e++fp+ +++ +dLl+++Lv+dp kRi+++eaLehpYl | Q: 252 ESPRAREYIKSLPMYPAAPLEKMFPRVNPKGIDLLQRMLVFDPAKRITAKEALEHPYL 309 | *********************************************************7 |
|
Domain Sequence
(FASTA) | | QLKSLLGEGA YGVVCSATHK PTGEIVAIKK IEPFDKPLFA LRTLREIKIL KHFKHENIIT 60 | | IFNIQRPDSF ENFNEVYIIQ ELMQTDLHRV ISTQMLSDDH IQYFIYQTLR AVKVLHGSNV 120 | | IHRDLKPSNL LINSNCDLKV CDFGLARIID ESAADNSEPT GQQSGMTEYV ATRWYRAPEV 180 | | MLTSAKYSRA MDVWSCGCIL AELFLRRPIF PGRDYRHQLL LIFGIIGTPH SDNDLRCIES 240 | | PRAREYIKSL PMYPAAPLEK MFPRVNPKGI DLLQRMLVFD PAKRITAKEA LEHPYL 296 |
|
| Keyword | 3D-structure; ATP-binding; Cell cycle; Cell division; Complete proteome; Conjugation; Cytoplasm; Kinase; Mitosis; Nucleotide-binding; Nucleus; Periplasm; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
| Prosites | |
| Prints | |
| Created Date | 20-Feb-2013 |