EKS-SAC-00026

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00026

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/CAMKL	232.3	1.9E-69	17	281	265

Status

Reviewed

Ensembl Protein

YBR274W

UniProt Accession

P38147; D6VQS0;

Protein Name

Serine/threonine-protein kinase CHK1

Protein Synonyms/Alias

Checkpoint kinase 1;

Gene Name

CHK1

Gene Synonyms/Alias

CHK1; YBR274W; YBR1742;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YBR274W	YBR274W	YBR274W

Organism

Saccharomyces cerevisiae

Functional Description

Serine/threonine-protein kinase which is required forcheckpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit.

Protein Length

527

Protein Sequence
(FASTA)

MSLSQVSPLP HIKDVVLGDT VGQGAFACVK NAHLQMDPSI ILAVKFIHVP TCKKMGLSDK 60

DITKEVVLQS KCSKHPNVLR LIDCNVSKEY MWIILEMADG GDLFDKIEPD VGVDSDVAQF 120

YFQQLVSAIN YLHVECGVAH RDIKPENILL DKNGNLKLAD FGLASQFRRK DGTLRVSMDQ 180

RGSPPYMAPE VLYSEEGYYA DRTDIWSIGI LLFVLLTGQT PWELPSLENE DFVFFIENDG 240

NLNWGPWSKI EFTHLNLLRK ILQPDPNKRV TLKALKLHPW VLRRASFSGD DGLCNDPELL 300

AKKLFSHLKV SLSNENYLKF TQDTNSNNRY ISTQPIGNEL AELEHDSMHF QTVSNTQRAF 360

TSYDSNTNYN SGTGMTQEAK WTQFISYDIA ALQFHSDEND CNELVKRHLQ FNPNKLTKFY 420

TLQPMDVLLP ILEKALNLSQ IRVKPDLFAN FERLCELLGY DNVFPLIINI KTKSNGGYQL 480

CGSISIIKIE EELKSVGFER KTGDPLEWRR LFKKISTICR DIILIPN 527

Nucleotide Sequence
(FASTA)

ATGAGTCTCT CGCAGGTGTC ACCTTTACCC CATATCAAGG ATGTTGTTTT AGGAGATACA 60

GTAGGCCAAG GCGCATTCGC CTGCGTTAAA AATGCTCATC TTCAAATGGA TCCCTCCATT 120

ATTCTAGCTG TTAAATTCAT TCATGTTCCC ACTTGCAAAA AAATGGGACT CAGTGACAAG 180

GATATCACAA AAGAGGTTGT TTTGCAATCG AAGTGTTCTA AGCATCCTAA TGTTTTGAGA 240

CTTATCGATT GTAATGTCTC TAAAGAATAT ATGTGGATAA TTCTGGAGAT GGCAGATGGT 300

GGTGATCTAT TTGATAAGAT TGAGCCTGAC GTTGGAGTTG ATTCCGACGT GGCCCAATTT 360

TACTTTCAAC AGCTCGTTAG TGCTATTAAT TATCTGCATG TAGAATGTGG AGTTGCTCAC 420

AGAGACATCA AGCCTGAAAA CATCTTACTC GATAAGAACG GAAATCTGAA GCTAGCTGAT 480

TTTGGGCTCG CCTCTCAATT TAGGAGGAAA GATGGTACAT TACGTGTATC CATGGATCAA 540

AGGGGTTCTC CACCCTACAT GGCTCCTGAA GTATTATATT CTGAAGAGGG TTATTACGCA 600

GATCGAACAG ATATATGGTC TATTGGCATC CTCTTGTTCG TATTGTTGAC TGGTCAAACG 660

CCTTGGGAAT TACCTTCATT AGAGAACGAA GATTTCGTCT TTTTTATTGA AAATGATGGA 720

AATTTAAACT GGGGACCCTG GTCAAAGATA GAATTTACTC ACTTGAACCT ACTCCGAAAA 780

ATTTTACAAC CTGACCCAAA TAAGAGGGTG ACATTGAAGG CTTTAAAGTT ACATCCTTGG 840

GTATTACGTC GAGCTTCATT TTCTGGGGAT GATGGTCTCT GTAATGACCC TGAACTCTTG 900

GCTAAGAAAC TGTTTTCTCA CTTAAAAGTC TCACTGAGTA ACGAAAATTA TTTGAAATTC 960

ACTCAAGATA CAAACTCTAA TAATAGGTAC ATTTCTACTC AGCCAATTGG TAACGAATTG 1020

GCTGAGCTTG AACACGACTC AATGCATTTC CAGACAGTTT CGAATACACA ACGTGCATTT 1080

ACCTCGTATG ATTCAAATAC GAACTATAAT AGCGGAACAG GTATGACACA AGAGGCTAAG 1140

TGGACGCAAT TCATAAGCTA TGATATCGCT GCCTTACAGT TTCATTCTGA TGAAAATGAT 1200

TGTAATGAAT TAGTAAAACG ACATTTACAA TTTAATCCGA ATAAACTTAC CAAGTTCTAC 1260

ACGTTGCAAC CTATGGATGT TTTGTTACCG ATTTTGGAGA AAGCCTTGAA TTTATCACAA 1320

ATTAGAGTAA AACCCGACCT TTTTGCGAAT TTTGAAAGAT TGTGCGAATT ATTGGGTTAT 1380

GATAACGTTT TCCCACTTAT TATAAATATT AAAACCAAAA GTAATGGGGG TTATCAATTA 1440

TGCGGTAGCA TTTCCATCAT AAAGATTGAA GAAGAGTTGA AAAGTGTCGG ATTTGAAAGA 1500

AAAACTGGTG ATCCTTTAGA ATGGAGAAGA TTGTTCAAGA AAATTTCAAC TATCTGTAGG 1560

GATATTATCC TAATTCCCAA CTGA 1584

Domain Profile

S: 3     llktlGegsfgkVklakhk.etkekvavKiikkkklsee..slekikrEieilkkl.khp  58

         l++t+G+g+f+ Vk a+ + +++  +avK+i+  + ++   s ++i++E+ +++k+ khp

Q: 17    LGDTVGQGAFACVKNAHLQmDPSIILAVKFIHVPTCKKMglSDKDITKEVVLQSKCsKHP  76

         6789***********9977589999*******9877555114569********99956**

S: 59    niikllevietkkklylvleyasggeLfdyieekgrlkekearklfkqlvsavkYlh.sk  117

         n+++l+++  +k++++++le+a+gg+Lfd+ie +  ++++ a+ +f+qlvsa++Ylh ++

Q: 77    NVLRLIDCNVSKEYMWIILEMADGGDLFDKIEPDVGVDSDVAQFYFQQLVSAINYLHvEC  136

         *********************************************************889

S: 118   givHrDlKpeNllldkkenikiaDFGlanlfkeg....klletfcGsppYaaPel.lkgk  172

         g++HrD+KpeN+lldk++n+k+aDFGla++f+++    ++  ++ GsppY+aPe+ ++++

Q: 137   GVAHRDIKPENILLDKNGNLKLADFGLASQFRRKdgtlRVSMDQRGSPPYMAPEVlYSEE  196

         *******************************9998888888899**********956779

S: 173   kYegpkvDvWslGvvLyalvtgklPfdee..nlkellekilk.gkleipkk..lskeles  227

          Y + ++D+Ws+G++L++l+tg++P++ +  ++++++  i++ g+l++  +  ++ +  +

Q: 197   GYYADRTDIWSIGILLFVLLTGQTPWELPslENEDFVFFIENdGNLNWGPWskIEFTHLN  256

         9**************************99554566666665516678887778999999*

S: 228   LlrklLvvdpekRatieeilkhewl  252

         Llrk+L+ dp+kR+t++ ++ h+w+

Q: 257   LLRKILQPDPNKRVTLKALKLHPWV  281

         ************************7

Domain Sequence
(FASTA)

LGDTVGQGAF ACVKNAHLQM DPSIILAVKF IHVPTCKKMG LSDKDITKEV VLQSKCSKHP 60

NVLRLIDCNV SKEYMWIILE MADGGDLFDK IEPDVGVDSD VAQFYFQQLV SAINYLHVEC 120

GVAHRDIKPE NILLDKNGNL KLADFGLASQ FRRKDGTLRV SMDQRGSPPY MAPEVLYSEE 180

GYYADRTDIW SIGILLFVLL TGQTPWELPS LENEDFVFFI ENDGNLNWGP WSKIEFTHLN 240

LLRKILQPDP NKRVTLKALK LHPWV 265

Keyword

ATP-binding; Cell cycle; Complete proteome; DNA damage; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Caenorhabditis elegans"; ?>Canis familiaris"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Pan troglodytes"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007049	; P:cell cycle
GO:0000077	; P:DNA damage checkpoint
GO:0048478	; P:replication fork protection

KEGG

sce:YBR274W

;

InterPros

IPR020636	; Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013