EKS-SAC-00050

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00050

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CAMK/RAD53	397.2	1.0E-119	200	480	281

Status

Reviewed

Ensembl Protein

YDL101C

UniProt Accession

P39009; D6VRP9;

Protein Name

DNA damage response protein kinase DUN1

Protein Synonyms/Alias

Gene Name

DUN1

Gene Synonyms/Alias

DUN1; YDL101C; D2370;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YDL101C	YDL101C	YDL101C

Organism

Saccharomyces cerevisiae

Functional Description

Transducer of the DNA damage signal. Phosphorylates SML1on serine residues. Cooperates with the PAN deadenylation complex in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress.

Protein Length

513

Protein Sequence
(FASTA)

MSLSTKREHS GDVTDSSFKR QQRSNKPSSE YTCLGHLVNL IPGKEQKVEI TNRNVTTIGR 60

SRSCDVILSE PDISTFHAEF HLLQMDVDNF QRNLINVIDK SRNGTFINGN RLVKKDYILK 120

NGDRIVFGKS CSFLFKYASS SSTDIENDDE KVSSESRSYK NDDEVFKKPQ ISATSSQNAT 180

TSAAIRKLNK TRPVSFFDKY LLGKELGAGH YALVKEAKNK KTGQQVAVKI FHAQQNDDQK 240

KNKQFREETN ILMRVQHPNI VNLLDSFVEP ISKSQIQKYL VLEKIDDGEL FERIVRKTCL 300

RQDESKALFK QLLTGLKYLH EQNIIHRDIK PENILLNITR RENPSQVQLG PWDEDEIDIQ 360

VKIADFGLAK FTGEMQFTNT LCGTPSYVAP EVLTKKGYTS KVDLWSAGVI LYVCLCGFPP 420

FSDQLGPPSL KEQILQAKYA FYSPYWDKID DSVLHLISNL LVLNPDERYN IDEALNHPWF 480

NDIQQQSSVS LELQRLQITD NKIPKTYSEL SCL 513

Nucleotide Sequence
(FASTA)

ATGAGTTTGT CCACGAAAAG AGAGCACTCT GGTGATGTAA CTGACTCTTC ATTCAAAAGA 60

CAGCAACGAA GTAATAAGCC CAGCTCAGAA TACACTTGTC TGGGCCATCT GGTGAACTTA 120

ATACCCGGAA AGGAGCAAAA AGTGGAAATA ACAAATAGAA ACGTTACTAC AATCGGTAGA 180

AGTAGGTCGT GCGACGTTAT ATTGAGTGAG CCTGACATCT CGACTTTCCA TGCTGAGTTT 240

CATCTACTGC AAATGGATGT GGATAATTTC CAAAGAAATT TGATTAATGT TATCGATAAG 300

AGCAGAAACG GAACTTTTAT TAATGGTAAT CGTTTGGTGA AGAAAGACTA CATCCTTAAG 360

AATGGTGACA GAATTGTCTT TGGCAAAAGT TGCTCCTTTT TGTTCAAGTA CGCATCTTCA 420

TCTTCTACTG ACATCGAAAA TGACGATGAA AAGGTAAGTT CAGAAAGTCG TTCATATAAG 480

AACGACGACG AAGTTTTCAA GAAACCGCAA ATAAGTGCTA CAAGTAGCCA AAATGCTACA 540

ACGAGTGCCG CAATTAGAAA ACTGAATAAG ACAAGGCCTG TGTCGTTCTT TGATAAATAT 600

TTATTAGGTA AAGAATTAGG TGCAGGGCAC TACGCCCTGG TGAAAGAAGC CAAAAACAAA 660

AAAACTGGTC AGCAAGTAGC TGTGAAAATA TTCCACGCTC AGCAAAATGA CGACCAAAAA 720

AAGAATAAAC AATTTAGGGA GGAAACTAAC ATCCTAATGA GAGTGCAACA TCCAAACATT 780

GTCAACTTAC TAGATAGTTT TGTAGAGCCA ATCAGCAAAT CTCAGATTCA AAAATATTTG 840

GTACTGGAAA AGATCGACGA TGGCGAACTA TTTGAAAGAA TCGTCAGAAA AACATGTTTG 900

AGACAGGATG AATCCAAAGC TCTATTCAAA CAGTTACTTA CCGGATTGAA GTATCTGCAT 960

GAGCAAAACA TCATTCACAG AGACATCAAG CCAGAAAATA TCTTATTAAA TATAACAAGG 1020

CGCGAAAATC CAAGTCAAGT CCAACTTGGC CCATGGGATG AAGATGAAAT TGATATTCAA 1080

GTTAAAATAG CGGATTTTGG CCTAGCAAAA TTTACAGGAG AAATGCAATT CACAAACACA 1140

CTTTGTGGTA CGCCTTCTTA TGTAGCGCCC GAAGTCCTCA CAAAGAAAGG ATACACATCA 1200

AAAGTAGATC TTTGGAGTGC TGGTGTCATA TTATATGTCT GTTTATGTGG TTTTCCTCCA 1260

TTCAGTGATC AATTGGGGCC ACCTTCATTG AAGGAACAGA TCTTACAAGC TAAGTATGCG 1320

TTTTACTCTC CGTACTGGGA TAAAATCGAT GACTCAGTAC TACATTTAAT ATCCAATCTT 1380

TTGGTCCTAA ATCCTGATGA AAGGTACAAT ATAGATGAAG CTTTGAACCA CCCTTGGTTC 1440

AATGACATAC AGCAACAAAG CTCGGTCTCA TTAGAATTGC AGCGTTTACA AATTACTGAC 1500

AATAAAATAC CCAAAACATA CTCAGAATTA TCTTGCCTCT AA 1542

Domain Profile

S: 1     yilskelGkGafgkvklayekktgkkvavkilskrksirssreikkakeveeeveilkkl  60

         y+l+kelG+G++++vk+a++kktg++vavki+++++    ++++kk+k+++ee++il+++

Q: 200   YLLGKELGAGHYALVKEAKNKKTGQQVAVKIFHAQQ----NDDQKKNKQFREETNILMRV  255

         7899********************************....********************

S: 61    shPnivklkdvfeeees.....kylvlelvegGelldkvvakkllkedlskllfkqllta  115

         +hPniv+l d+f+e+ s     kylvle++++Gel++++v+k++l++d+sk+lfkqllt+

Q: 256   QHPNIVNLLDSFVEPISksqiqKYLVLEKIDDGELFERIVRKTCLRQDESKALFKQLLTG  315

         **************999889999*************************************

S: 116   vkylhekgiihrDlkPeniLl................sekeedvlvkitDfGlakvlgev  159

         +kylhe++iihrD+kPeniLl                +e+e+d++vki+DfGlak++ge+

Q: 316   LKYLHEQNIIHRDIKPENILLnitrrenpsqvqlgpwDEDEIDIQVKIADFGLAKFTGEM  375

         ************************************************************

S: 160   slmktlCGtpsyvaPevlvkkgraeytskvDiWslGvvlfvilsGypPfseeltqvslke  219

         ++++tlCGtpsyvaPevl+kkg   ytskvD+Ws+Gv+l+v+l+G+pPfs++l+++slke

Q: 376   QFTNTLCGTPSYVAPEVLTKKG---YTSKVDLWSAGVILYVCLCGFPPFSDQLGPPSLKE  432

         **********************...***********************************

S: 220   qilkgkyafieekwkkiseeakdlikklLvvdPeerlsieealnhpWl  267

         qil++kyaf++++w+ki++++++li++lLv++P+er++i+ealnhpW+

Q: 433   QILQAKYAFYSPYWDKIDDSVLHLISNLLVLNPDERYNIDEALNHPWF  480

         ***********************************************7

Domain Sequence
(FASTA)

YLLGKELGAG HYALVKEAKN KKTGQQVAVK IFHAQQNDDQ KKNKQFREET NILMRVQHPN 60

IVNLLDSFVE PISKSQIQKY LVLEKIDDGE LFERIVRKTC LRQDESKALF KQLLTGLKYL 120

HEQNIIHRDI KPENILLNIT RRENPSQVQL GPWDEDEIDI QVKIADFGLA KFTGEMQFTN 180

TLCGTPSYVA PEVLTKKGYT SKVDLWSAGV ILYVCLCGFP PFSDQLGPPS LKEQILQAKY 240

AFYSPYWDKI DDSVLHLISN LLVLNPDERY NIDEALNHPW F 281

Keyword

3D-structure; ATP-binding; Complete proteome; DNA damage; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Callithrix jacchus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gallus gallus"; ?>Homo sapiens"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>

Gene Ontology

GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004672	; F:protein kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0000077	; P:DNA damage checkpoint
GO:0048478	; P:replication fork protection

KEGG

sce:YDL101C

;

InterPros

IPR000253	; FHA_dom.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.
IPR008984	; SMAD_FHA_domain.

Pfam

PF00498	; FHA; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00240	; FHA; 1.
SM00220	; S_TKc; 1.

Prosites

PS50006	; FHA_DOMAIN; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013