EKS-SAC-00083

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00083

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/PEK	334.5	1.7E-100	599	981	383

Status

Reviewed

Ensembl Protein

YDR283C

UniProt Accession

P15442; D6VSR3;

Protein Name

Serine/threonine-protein kinase GCN2

Protein Synonyms/Alias

Gene Name

GCN2

Gene Synonyms/Alias

GCN2; AAS1; YDR283C;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YDR283C	YDR283C	YDR283C

Organism

Saccharomyces cerevisiae

Functional Description

Stimulates GCN4 translation in amino acid-starved cellsby phosphorylating the alpha subunit of eIF-2 (SUI2) on 'Ser-52'.

Protein Length

1659

Protein Sequence
(FASTA)

MSLSHLTLDQ YYEIQCNELE AIRSIYMDDF TDLTKRKSSW DKQPQIIFEI TLRSVDKEPV 60

ESSITLHFAM TPMYPYTAPE IEFKNVQNVM DSQLQMLKSE FKKIHNTSRG QEIIFEITSF 120

TQEKLDEFQN VVNTQSLEDD RLQRIKETKE QLEKEEREKQ QETIKKRSDE QRRIDEIVQR 180

ELEKRQDDDD DLLFNRTTQL DLQPPSEWVA SGEAIVFSKT IKAKLPNNSM FKFKAVVNPK 240

PIKLTSDIFS FSKQFLVKPY IPPESPLADF LMSSEMMENF YYLLSEIELD NSYFNTSNGK 300

KEIANLEKEL ETVLKAKHDN VNRLFGYTVE RMGRNNATFV WKIRLLTEYC NYYPLGDLIQ 360

SVGFVNLATA RIWMIRLLEG LEAIHKLGIV HKCINLETVI LVKDADFGST IPKLVHSTYG 420

YTVLNMLSRY PNKNGSSVEL SPSTWIAPEL LKFNNAKPQR LTDIWQLGVL FIQIISGSDI 480

VMNFETPQEF LDSTSMDETL YDLLSKMLNN DPKKRLGTLE LLPMKFLRTN IDSTINRFNL 540

VSESVNSNSL ELTPGDTITV RGNGGRTLSQ SSIRRRSFNV GSRFSSINPA TRSRYASDFE 600

EIAVLGQGAF GQVVKARNAL DSRYYAIKKI RHTEEKLSTI LSEVMLLASL NHQYVVRYYA 660

AWLEEDSMDE NVFESTDEES DLSESSSDFE ENDLLDQSSI FKNRTNHDLD NSNWDFISGS 720

GYPDIVFENS SRDDENEDLD HDTSSTSSSE SQDDTDKESK SIQNVPRRRN FVKPMTAVKK 780

KSTLFIQMEY CENRTLYDLI HSENLNQQRD EYWRLFRQIL EALSYIHSQG IIHRDLKPMN 840

IFIDESRNVK IGDFGLAKNV HRSLDILKLD SQNLPGSSDN LTSAIGTAMY VATEVLDGTG 900

HYNEKIDMYS LGIIFFEMIY PFSTGMERVN ILKKLRSVSI EFPPDFDDNK MKVEKKIIRL 960

LIDHDPNKRP GARTLLNSGW LPVKHQDEVI KEALKSLSNP SSPWQQQVRE SLFNQSYSLT 1020

NDILFDNSVP TSTPFANILR SQMTEEVVKI FRKHGGIENN APPRIFPKAP IYGTQNVYEV 1080

LDKGGTVLQL QYDLTYPMAR YLSKNPSLIS KQYRMQHVYR PPDHSRSSLE PRKFGEIDFD 1140

IISKSSSESG FYDAESLKII DEILTVFPVF EKTNTFFILN HADILESVFN FTNIDKAQRP 1200

LVSRMLSQVG FARSFKEVKN ELKAQLNISS TALNDLELFD FRLDFEAAKK RLYKLMIDSP 1260

HLKKIEDSLS HISKVLSYLK PLEVARNVVI SPLSNYNSAF YKGGIMFHAV YDDGSSRNMI 1320

AAGGRYDTLI SFFARPSGKK SSNTRKAVGF NLAWETIFGI AQNYFKLASG NRIKKRNRFL 1380

KDTAVDWKPS RCDVLISSFS NSLLDTIGVT ILNTLWKQNI KADMLRDCSS VDDVVTGAQQ 1440

DGIDWILLIK QQAYPLTNHK RKYKPLKIKK LSTNVDIDLD LDEFLTLYQQ ETGNKSLIND 1500

SLTLGDKADE FKRWDENSSA GSSQEGDIDD VVAGSTNNQK VIYVPNMATR SKKANKREKW 1560

VYEDAARNSS NMILHNLSNA PIITVDALRD ETLEIISITS LAQKEEWLRK VFGSGNNSTP 1620

RSFATSIYNN LSKEAHKGNR WAILYCHKTG KSSVIDLQR 1659

Nucleotide Sequence
(FASTA)

ATGTCATTGA GTCATCTCAC TTTAGATCAA TATTATGAAA TACAGTGCAA CGAACTTGAA 60

GCGATACGTT CCATTTATAT GGATGACTTT ACTGACTTAA CTAAAAGAAA GTCTAGCTGG 120

GATAAGCAGC CACAGATTAT ATTCGAAATT ACGCTTCGAT CTGTTGACAA AGAGCCGGTT 180

GAATCTTCTA TAACATTACA TTTTGCGATG ACCCCAATGT ATCCTTATAC CGCTCCAGAA 240

ATAGAATTCA AAAATGTACA AAATGTAATG GATAGTCAAT TGCAAATGCT GAAAAGTGAA 300

TTTAAGAAAA TCCACAACAC CTCCCGAGGC CAAGAGATTA TATTTGAAAT TACATCTTTT 360

ACTCAAGAAA AACTGGACGA ATTTCAAAAT GTGGTAAATA CACAGTCCTT GGAAGATGAT 420

CGATTACAAA GAATCAAAGA AACTAAAGAA CAATTAGAAA AGGAAGAAAG AGAAAAACAA 480

CAGGAAACAA TAAAAAAAAG ATCAGATGAG CAGCGAAGGA TAGATGAAAT TGTTCAAAGA 540

GAGTTGGAGA AAAGACAAGA TGATGATGAT GATTTGCTAT TCAACAGAAC AACCCAGTTA 600

GATTTACAAC CACCTTCAGA ATGGGTTGCA TCAGGTGAAG CTATTGTCTT TTCAAAAACT 660

ATAAAGGCAA AATTGCCTAA TAACTCAATG TTCAAGTTTA AAGCAGTTGT AAATCCTAAG 720

CCAATAAAAC TGACATCAGA TATATTTAGT TTTTCCAAAC AATTTCTTGT GAAGCCTTAT 780

ATACCACCAG AATCTCCGTT GGCAGATTTT TTAATGTCTT CTGAAATGAT GGAAAATTTC 840

TACTATTTGC TATCTGAAAT TGAATTGGAT AATAGCTATT TCAACACAAG TAATGGAAAA 900

AAAGAAATAG CAAATTTAGA GAAAGAGTTA GAGACGGTGT TGAAAGCTAA GCATGACAAT 960

GTGAATCGAT TGTTCGGTTA TACGGTGGAG CGCATGGGGA GAAATAATGC AACGTTTGTT 1020

TGGAAAATAA GACTCTTGAC AGAGTACTGT AACTACTATC CATTGGGAGA TTTGATACAA 1080

TCTGTTGGAT TTGTTAACTT AGCAACAGCG CGTATTTGGA TGATTAGATT GCTTGAAGGA 1140

TTGGAGGCCA TACACAAATT GGGAATTGTT CATAAATGTA TCAACTTAGA AACCGTGATC 1200

CTGGTGAAGG ATGCAGATTT TGGAAGCACT ATTCCCAAGT TAGTTCACTC CACTTATGGC 1260

TACACTGTTT TGAATATGCT ATCGAGATAT CCAAATAAAA ATGGTTCTTC GGTTGAGTTA 1320

TCTCCAAGTA CATGGATAGC CCCTGAGTTG TTGAAATTCA ATAACGCCAA ACCTCAAAGA 1380

TTAACTGATA TTTGGCAACT TGGTGTTTTG TTTATCCAGA TAATCAGTGG ATCTGATATA 1440

GTGATGAATT TTGAAACGCC TCAAGAATTC CTAGATTCAA CAAGTATGGA TGAAACTTTA 1500

TATGATCTTC TTTCGAAAAT GCTTAATAAC GATCCGAAGA AAAGATTAGG AACATTAGAA 1560

CTACTGCCCA TGAAATTCTT AAGGACCAAT ATTGACTCTA CAATCAATCG ATTTAACTTA 1620

GTTTCCGAAA GTGTCAATTC TAATTCCTTG GAGTTAACTC CTGGAGATAC CATAACCGTT 1680

CGGGGCAATG GAGGTAGAAC ACTTTCACAA TCGAGTATAC GAAGAAGATC ATTTAATGTT 1740

GGTTCCAGAT TCTCTTCTAT AAATCCTGCA ACGCGATCAC GATATGCTTC TGACTTTGAA 1800

GAGATTGCAG TTTTAGGCCA GGGCGCATTT GGACAAGTTG TCAAGGCACG TAATGCTCTC 1860

GATAGCAGAT ACTATGCGAT CAAGAAGATT AGACATACAG AAGAAAAGTT ATCTACTATA 1920

TTGAGTGAAG TAATGCTGTT AGCAAGCTTA AATCATCAAT ATGTTGTGCG TTACTATGCT 1980

GCATGGTTAG AAGAAGACAG TATGGATGAA AACGTTTTTG AATCAACTGA TGAAGAAAGT 2040

GACTTGAGCG AATCTTCCTC TGATTTTGAG GAAAATGATT TATTAGATCA AAGCAGTATT 2100

TTTAAAAATA GAACAAATCA CGATTTGGAT AATAGTAACT GGGATTTCAT ATCGGGGTCA 2160

GGATATCCGG ATATTGTCTT TGAAAATAGT TCTCGTGATG ATGAAAATGA AGATCTAGAC 2220

CATGATACTT CCTCGACTTC CTCGAGCGAA AGTCAAGATG ATACTGATAA AGAATCAAAG 2280

AGTATCCAGA ACGTTCCAAG AAGGAGGAAT TTTGTAAAAC CGATGACTGC TGTTAAGAAG 2340

AAAAGTACGC TTTTTATTCA AATGGAGTAC TGTGAAAATA GAACGCTATA TGATTTGATC 2400

CATTCTGAAA ATTTAAATCA ACAACGTGAT GAATATTGGA GGTTATTTCG ACAAATTTTG 2460

GAAGCACTGA GTTATATACA TTCCCAGGGT ATCATTCATA GGGATCTGAA GCCAATGAAT 2520

ATTTTTATAG ATGAATCGAG AAATGTTAAA ATCGGTGATT TTGGGTTAGC TAAGAACGTC 2580

CATAGATCTC TGGATATACT TAAGCTAGAT TCACAGAATT TGCCAGGCAG CTCAGATAAT 2640

TTAACATCCG CCATTGGTAC AGCAATGTAT GTTGCTACTG AAGTTTTAGA TGGTACAGGT 2700

CACTATAATG AAAAGATTGA TATGTATTCA CTTGGAATCA TTTTTTTTGA AATGATCTAT 2760

CCTTTCAGTA CAGGTATGGA GAGAGTTAAT ATTTTGAAAA AGTTACGATC AGTGTCGATA 2820

GAATTTCCTC CTGATTTCGA CGATAATAAG ATGAAAGTTG AAAAGAAAAT TATAAGGTTA 2880

CTCATAGACC ATGATCCCAA TAAAAGGCCT GGTGCTAGGA CATTATTAAA TAGTGGTTGG 2940

CTTCCTGTGA AGCATCAGGA TGAAGTAATC AAAGAGGCTT TAAAAAGTTT GTCGAATCCT 3000

TCATCCCCTT GGCAACAGCA AGTTCGAGAA AGTTTATTTA ACCAATCTTA CAGTCTAACA 3060

AATGATATTC TATTTGATAA CTCAGTTCCA ACATCCACTC CTTTCGCAAA CATTCTCAGG 3120

TCCCAAATGA CAGAAGAGGT AGTTAAAATT TTCAGGAAAC ATGGAGGAAT TGAAAATAAT 3180

GCTCCTCCGA GGATTTTTCC AAAGGCCCCC ATATACGGTA CGCAGAATGT ATATGAAGTG 3240

CTTGACAAGG GCGGTACCGT CTTGCAGTTA CAATATGATT TAACTTATCC TATGGCTAGG 3300

TATCTATCTA AAAATCCAAG TCTGATTTCT AAGCAATATA GGATGCAGCA CGTTTACCGA 3360

CCTCCTGATC ATTCAAGGTC AAGTTTGGAA CCTAGAAAGT TTGGTGAGAT TGACTTCGAC 3420

ATAATTTCAA AATCTTCCTC AGAGTCAGGA TTTTATGATG CAGAAAGCTT GAAAATTATC 3480

GATGAAATAT TAACCGTATT TCCTGTATTT GAGAAAACAA ACACTTTTTT CATATTAAAT 3540

CATGCTGATA TTTTGGAGAG TGTTTTCAAC TTTACAAATA TTGATAAAGC CCAAAGGCCT 3600

CTAGTTTCAC GAATGTTGTC GCAAGTAGGC TTTGCAAGGT CCTTCAAGGA AGTAAAGAAT 3660

GAACTAAAGG CGCAACTGAA CATATCTTCT ACGGCATTGA ATGATTTGGA GTTATTTGAT 3720

TTTAGACTGG ACTTCGAAGC AGCCAAAAAA CGCCTGTATA AATTGATGAT TGATAGTCCG 3780

CATCTAAAAA AAATTGAGGA CTCTTTGTCC CATATATCAA AGGTTCTCAG TTACCTAAAA 3840

CCCTTAGAAG TTGCAAGAAA TGTTGTGATA TCTCCTTTGA GTAACTACAA TAGCGCTTTT 3900

TACAAAGGAG GTATCATGTT TCATGCAGTT TATGACGATG GATCCTCACG TAATATGATA 3960

GCTGCTGGAG GGAGGTATGA CACTTTGATA TCCTTTTTTG CCAGACCATC AGGAAAAAAG 4020

AGCAGCAATA CTCGTAAGGC TGTAGGTTTC AACTTAGCGT GGGAAACAAT ATTCGGTATA 4080

GCCCAAAACT ATTTCAAACT CGCTTCTGGA AATAGGATAA AGAAGAGAAA TAGGTTTTTG 4140

AAAGATACAG CTGTTGATTG GAAGCCAAGC AGGTGTGATG TATTGATATC GAGTTTTTCG 4200

AACTCTTTGT TGGACACAAT CGGGGTTACA ATACTGAATA CATTGTGGAA GCAAAACATT 4260

AAAGCGGATA TGTTAAGGGA TTGTTCCTCG GTGGATGATG TCGTTACTGG CGCTCAACAG 4320

GATGGTATAG ACTGGATTTT GCTGATTAAG CAACAAGCGT ATCCACTAAC CAATCACAAG 4380

AGAAAGTACA AGCCATTAAA AATAAAAAAA TTGAGCACTA ATGTTGACAT AGATTTAGAT 4440

CTTGATGAGT TTTTAACCTT GTACCAACAA GAAACTGGTA ATAAATCTTT GATCAACGAT 4500

AGTCTCACTT TGGGCGATAA GGCTGATGAA TTTAAAAGAT GGGATGAAAA CAGCAGTGCC 4560

GGTAGTAGTC AAGAAGGTGA CATAGATGAT GTTGTTGCTG GTTCGACTAA TAATCAAAAG 4620

GTAATTTATG TTCCAAACAT GGCTACAAGA TCTAAGAAAG CTAATAAAAG GGAAAAGTGG 4680

GTTTATGAGG ATGCAGCCAG AAATTCTTCG AATATGATAT TACACAATTT ATCCAATGCA 4740

CCAATTATCA CTGTTGATGC CTTAAGAGAT GAAACTTTAG AAATAATCTC AATTACTTCT 4800

TTGGCTCAGA AGGAAGAATG GCTGAGAAAA GTTTTTGGGT CAGGTAATAA CTCGACTCCT 4860

AGAAGCTTTG CCACGAGCAT TTATAATAAC CTCTCCAAAG AGGCTCATAA AGGGAATAGG 4920

TGGGCAATAT TATACTGCCA CAAAACCGGA AAATCATCTG TTATCGATTT ACAGAGGTAG 4980

Domain Profile

S: 1     feeikvlGkGgfgvVlkaknkldkkeyaikriklnkakkelekverevkaLaklehpniv  60

         feei+vlG+G+fg+V+ka+n ld+++yaik+i+++++k  l+++++ev++La+l+h+ +v

Q: 599   FEEIAVLGQGAFGQVVKARNALDSRYYAIKKIRHTEEK--LSTILSEVMLLASLNHQYVV  656

         89*******************************99966..********************

S: 61    rYfaawleekeesl..............................................  74

         rY+aawlee++

Q: 657   RYYAAWLEEDSMDEnvfestdeesdlsesssdfeendlldqssifknrtnhdldnsnwdf  716

         *********997776677777777777777777777777777777777777777777777

S: 75    ................tdsseveaeaeleesesseesdtk....................  98

                         +                      +

Q: 717   isgsgypdivfenssrD----------------------Denedldhdtsstsssesqdd  754

         77777777777766542......................033333333333333333334

S: 99    ............................llyiqmelceketLkdwiderkllesrselvm  130

                                      l+iqme+ce++tL d+i++++l+++r+e++

Q: 755   tdkesksiqnvprrrnfvkpmtavkkksTLFIQMEYCENRTLYDLIHSENLNQQRDEYW-  813

         4478889999999**********************************************.

S: 131   rlfkqllegleylHskgliHrdLkpsniFldsddqvKigDFgLatala...ealkedvkq  187

         rlf+q+le+l+y+Hs+g+iHrdLkp+niF+d++++vKigDFgLa++ +   ++lk d+++

Q: 814   RLFRQILEALSYIHSQGIIHRDLKPMNIFIDESRNVKIGDFGLAKNVHrslDILKLDSQN  873

         *********************************************999555667788888

S: 188   daqskeakhtgqvgtklymsPeql.eksqYnkkvDifsLGlilfeLlypfstemervetl  246

          + s++ + t+ +gt++y+++e+l  + +Yn+k+D++sLG+i+fe++ypfst+merv++l

Q: 874   LPGSSD-NLTSAIGTAMYVATEVLdGTGHYNEKIDMYSLGIIFFEMIYPFSTGMERVNIL  932

         777777.*****************7789********************************

S: 247   telrd..gkipedfdd.kvpkekkllqkllsrdpkeRpsasellksell  292

         ++lr+  +++p dfdd k+++ekk+++ l+++dp++Rp a++ll+s +l

Q: 933   KKLRSvsIEFPPDFDDnKMKVEKKIIRLLIDHDPNKRPGARTLLNSGWL  981

         *****99*******999***************************99875

Domain Sequence
(FASTA)

FEEIAVLGQG AFGQVVKARN ALDSRYYAIK KIRHTEEKLS TILSEVMLLA SLNHQYVVRY 60

YAAWLEEDSM DENVFESTDE ESDLSESSSD FEENDLLDQS SIFKNRTNHD LDNSNWDFIS 120

GSGYPDIVFE NSSRDDENED LDHDTSSTSS SESQDDTDKE SKSIQNVPRR RNFVKPMTAV 180

KKKSTLFIQM EYCENRTLYD LIHSENLNQQ RDEYWRLFRQ ILEALSYIHS QGIIHRDLKP 240

MNIFIDESRN VKIGDFGLAK NVHRSLDILK LDSQNLPGSS DNLTSAIGTA MYVATEVLDG 300

TGHYNEKIDM YSLGIIFFEM IYPFSTGMER VNILKKLRSV SIEFPPDFDD NKMKVEKKII 360

RLLIDHDPNK RPGARTLLNS GWL 383

Keyword

3D-structure; Amino-acid biosynthesis; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Kinase; Ligase; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; Serine/threonine-protein kinase; Stress response; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Equus caballus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0022626	; C:cytosolic ribosome
GO:0004812	; F:aminoacyl-tRNA ligase activity
GO:0005524	; F:ATP binding
GO:0004694	; F:eukaryotic translation initiation factor 2alpha kinase activity
GO:0042803	; F:protein homodimerization activity
GO:0000049	; F:tRNA binding
GO:0008652	; P:cellular amino acid biosynthetic process
GO:0000077	; P:DNA damage checkpoint
GO:0006446	; P:regulation of translational initiation
GO:0006412	; P:translation

KEGG

sce:YDR283C

;

InterPros

IPR024435	; GCN2_anticodon-bd_dom.
IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR006575	; RWD-domain.
IPR008271	; Ser/Thr_kinase_AS.
IPR016255	; Ser/Thr_kinase_GCN2.
IPR016135	; UBQ-conjugating_enzyme/RWD.

Pfam

PF12745	; HGTP_anticodon2; 1.
PF00069	; Pkinase; 3.
PF05773	; RWD; 1.

SMARTs

SM00591

; RWD; 1.

Prosites

PS50862	; AA_TRNA_LIGASE_II; FALSE_NEG.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 2.
PS00108	; PROTEIN_KINASE_ST; 1.
PS50908	; RWD; 1.

Prints

Created Date

20-Feb-2013