EPS-SAC-00010

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-SAC-00010

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
PPP/PP5	584.4	1.4E-176	188	513	326

Status

Reviewed

Ensembl Protein

YGR123C

UniProt Accession

P53043; D6VUQ5;

Protein Name

Serine/threonine-protein phosphatase T

Protein Synonyms/Alias

PPT;

Gene Name

PPT1

Gene Synonyms/Alias

PPT1; YGR123C; G6347;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YGR123C	YGR123C	YGR123C

Organism

Saccharomyces cerevisiae

Functional Description

Protein phosphatase that specifically binds to anddephosphorylates the molecular chaperone Hsp90 (HSC82 and HSP82). Dephosphorylation positively regulates the Hsp90 chaperone machinery.

Protein Length

513

Protein Sequence
(FASTA)

MSTPTAADRA KALERKNEGN VFVKEKHFLK AIEKYTEAID LDSTQSIYFS NRAFAHFKVD 60

NFQSALNDCD EAIKLDPKNI KAYHRRALSC MALLEFKKAR KDLNVLLKAK PNDPAATKAL 120

LTCDRFIREE RFRKAIGGAE NEAKISLCQT LNLSSFDANA DLANYEGPKL EFEQLYDDKN 180

AFKGAKIKNM SQEFISKMVN DLFLKGKYLP KKYVAAIISH ADTLFRQEPS MVELENNSTP 240

DVKISVCGDT HGQFYDVLNL FRKFGKVGPK HTYLFNGDFV DRGSWSCEVA LLFYCLKILH 300

PNNFFLNRGN HESDNMNKIY GFEDECKYKY SQRIFNMFAQ SFESLPLATL INNDYLVMHG 360

GLPSDPSATL SDFKNIDRFA QPPRDGAFME LLWADPQEAN GMGPSQRGLG HAFGPDITDR 420

FLRNNKLRKI FRSHELRMGG VQFEQKGKLM TVFSAPNYCD SQGNLGGVIH VVPGHGILQA 480

GRNDDQNLII ETFEAVEHPD IKPMAYSNGG FGL 513

Nucleotide Sequence
(FASTA)

ATGTCAACAC CCACAGCAGC AGATCGCGCG AAGGCACTTG AAAGAAAGAA CGAAGGAAAT 60

GTTTTTGTGA AGGAAAAACA TTTTTTAAAG GCCATTGAAA AATATACAGA GGCGATCGAT 120

TTAGATTCTA CACAGTCTAT CTATTTTTCG AACAGAGCAT TTGCTCACTT TAAGGTAGAT 180

AACTTTCAAA GCGCTTTAAA TGACTGTGAT GAAGCCATTA AACTGGATCC CAAAAATATC 240

AAAGCTTATC ATAGACGTGC ATTATCCTGC ATGGCTCTGC TAGAATTTAA AAAGGCTAGG 300

AAAGATTTGA ATGTGCTTCT AAAGGCGAAG CCCAATGATC CGGCTGCTAC TAAGGCGTTA 360

CTTACATGTG ATAGGTTCAT TAGAGAAGAA AGATTTAGGA AAGCTATCGG AGGCGCAGAA 420

AATGAAGCCA AGATCAGTTT GTGTCAGACT TTGAACTTGA GTTCATTTGA TGCTAATGCA 480

GATTTAGCCA ATTATGAAGG TCCAAAGTTA GAATTTGAAC AGCTGTATGA CGATAAGAAT 540

GCCTTTAAAG GTGCAAAGAT CAAGAACATG TCTCAAGAGT TTATTTCCAA AATGGTTAAC 600

GATCTTTTCC TAAAGGGAAA ATATTTACCA AAAAAGTACG TTGCAGCTAT TATCTCTCAT 660

GCTGATACCT TATTTCGTCA AGAGCCATCT ATGGTAGAAC TAGAAAATAA CTCTACTCCT 720

GATGTTAAGA TATCAGTATG TGGTGATACG CATGGCCAAT TTTATGATGT TTTGAATTTA 780

TTTCGCAAGT TTGGTAAAGT AGGTCCTAAG CACACTTATC TATTCAATGG TGATTTTGTC 840

GACCGTGGTT CTTGGTCGTG TGAAGTTGCA CTATTATTCT ACTGTTTGAA AATATTACAT 900

CCAAATAATT TCTTTTTGAA CAGAGGTAAT CATGAGAGTG ATAATATGAA CAAAATCTAT 960

GGGTTTGAAG ATGAGTGTAA ATACAAATAT TCACAAAGGA TATTTAACAT GTTTGCACAA 1020

AGTTTTGAGA GCCTTCCATT GGCTACCTTA ATTAATAATG ATTACCTTGT AATGCATGGT 1080

GGTTTACCAA GTGATCCTTC AGCAACTTTG TCTGATTTCA AGAATATTGA TAGATTTGCC 1140

CAACCACCTA GAGATGGCGC CTTTATGGAA CTTCTATGGG CGGACCCACA AGAAGCCAAC 1200

GGGATGGGTC CTTCTCAGCG TGGTTTAGGA CATGCTTTTG GACCCGACAT TACAGATAGA 1260

TTTCTAAGAA ACAACAAATT GCGCAAGATT TTTAGATCTC ACGAGTTAAG AATGGGTGGT 1320

GTGCAATTCG AGCAGAAGGG TAAATTAATG ACAGTTTTCA GTGCTCCAAA CTATTGTGAT 1380

AGCCAAGGTA ATCTTGGGGG TGTTATTCAT GTTGTACCAG GCCATGGTAT TTTACAGGCT 1440

GGCAGGAATG ACGACCAAAA TCTAATAATT GAGACATTCG AAGCTGTTGA GCACCCAGAT 1500

ATAAAACCAA TGGCCTATTC TAATGGTGGT TTTGGTTTAT AG 1542

Domain Profile

S: 1     kkvsiefikklvndlklkkkllrkkavailvsvadvlfkle...velennskedvkitvc  57

         k++s+efi+k+vndl+lk+k+l+kk+va+++s+ad+lf++e   velenns++dvki+vc

Q: 188   KNMSQEFISKMVNDLFLKGKYLPKKYVAAIISHADTLFRQEpsmVELENNSTPDVKISVC  247

         69**************************************99999***************

S: 58    GdthGqfydllnifelnGlpseknpylfnGdfvdrGsfsveviltlfglkllyPdhffll  117

         GdthGqfyd+ln+f+++G++++k++ylfnGdfvdrGs+s+ev+l++++lk+l+P++ffl+

Q: 248   GDTHGQFYDVLNLFRKFGKVGPKHTYLFNGDFVDRGSWSCEVALLFYCLKILHPNNFFLN  307

         ************************************************************

S: 118   rGnhesdnmnkiyGfegevkakytaklyelfaevfewlPlaqlingkvlvmhGGlfsedg  177

         rGnhesdnmnkiyGfe+e+k+ky+++++++fa++fe+lPla+lin+++lvmhGGl+s+++

Q: 308   RGNHESDNMNKIYGFEDECKYKYSQRIFNMFAQSFESLPLATLINNDYLVMHGGLPSDPS  367

         ************************************************************

S: 178   vtlddirkiernaqPPeeGalcellWsdPqeanGrgvskrGvglqfGpdvtkrfleenkl  237

         +tl+d+++i+r+aqPP++Ga++ellW+dPqeanG+g+s+rG+g++fGpd+t+rfl++nkl

Q: 368   ATLSDFKNIDRFAQPPRDGAFMELLWADPQEANGMGPSQRGLGHAFGPDITDRFLRNNKL  427

         ************************************************************

S: 238   dyiirshevkaegveveqkgklvtvfsapnycdsqgnlasvihv..........rnddlr  287

         ++i+rshe++++gv++eqkgkl+tvfsapnycdsqgnl++vihv          rndd++

Q: 428   RKIFRSHELRMGGVQFEQKGKLMTVFSAPNYCDSQGNLGGVIHVvpghgilqagRNDDQN  487

         ************************************************************

S: 288   liietfeavehpdvkpmaysngllgl  313

         liietfeavehpd+kpmaysng++gl

Q: 488   LIIETFEAVEHPDIKPMAYSNGGFGL  513

         ***********************986

Domain Sequence
(FASTA)

KNMSQEFISK MVNDLFLKGK YLPKKYVAAI ISHADTLFRQ EPSMVELENN STPDVKISVC 60

GDTHGQFYDV LNLFRKFGKV GPKHTYLFNG DFVDRGSWSC EVALLFYCLK ILHPNNFFLN 120

RGNHESDNMN KIYGFEDECK YKYSQRIFNM FAQSFESLPL ATLINNDYLV MHGGLPSDPS 180

ATLSDFKNID RFAQPPRDGA FMELLWADPQ EANGMGPSQR GLGHAFGPDI TDRFLRNNKL 240

RKIFRSHELR MGGVQFEQKG KLMTVFSAPN YCDSQGNLGG VIHVVPGHGI LQAGRNDDQN 300

LIIETFEAVE HPDIKPMAYS NGGFGL 326

Keyword

3D-structure; Complete proteome; Hydrolase; Iron; Manganese; Metal-binding; Nucleus; Protein phosphatase; Reference proteome; Repeat; TPR repeat.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0046872	; F:metal ion binding
GO:0004722	; F:protein serine/threonine phosphatase activity
GO:0006470	; P:protein dephosphorylation

KEGG

sce:YGR123C

;

InterPros

IPR004843	; Metallo_PEstase_dom.
IPR013235	; PPP_dom.
IPR006186	; Ser/Thr-sp_prot-phosphatase.
IPR011236	; Ser/Thr_PPase_5.
IPR001440	; TPR-1.
IPR013026	; TPR-contain_dom.
IPR011990	; TPR-like_helical.
IPR013105	; TPR_2.
IPR019734	; TPR_repeat.

Pfam

PF00149	; Metallophos; 1.
PF08321	; PPP5; 1.
PF00515	; TPR_1; 1.
PF07719	; TPR_2; 1.

SMARTs

SM00156	; PP2Ac; 1.
SM00028	; TPR; 3.

Prosites

PS00125	; SER_THR_PHOSPHATASE; 1.
PS50005	; TPR; 3.
PS50293	; TPR_REGION; 1.

Prints

PR00114

; STPHPHTASE.

Created Date

20-Feb-2013