EKS-SAC-00063

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00063

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/BUB	386.0	2.4E-116	705	980	276

Status

Reviewed

Ensembl Protein

YGR188C

UniProt Accession

P41695; D6VUX1;

Protein Name

Checkpoint serine/threonine-protein kinase BUB1

Protein Synonyms/Alias

Gene Name

BUB1

Gene Synonyms/Alias

BUB1; YGR188C; G7542;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YGR188C	YGR188C	YGR188C

Organism

Saccharomyces cerevisiae

Functional Description

Involved in cell cycle checkpoint enforcement. Theformation of a MAD1-BUB1-BUB3 complex seems to be required for the spindle checkpoint mechanism. Catalyzes the phosphorylation of BUB3 and its autophosphorylation. Associates with centromere (CEN) DNA via interaction with SKP1. The association with SKP1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle depolymerization or kinetochore assembly defects.

Protein Length

1021

Protein Sequence
(FASTA)

MNLDLGSTVR GYESDKDTFP QSKGVSSSQK EQHSQLNQTK IAYEQRLLND LEDMDDPLDL 60

FLDYMIWIST SYIEVDSESG QEVLRSTMER CLIYIQDMET YRNDPRFLKI WIWYINLFLS 120

NNFHESENTF KYMFNKGIGT KLSLFYEEFS KLLENAQFFL EAKVLLELGA ENNCRPYNRL 180

LRSLSNYEDR LREMNIVENQ NSVPDSRERL KGRLIYRTAP FFIRKFLTSS LMTDDKENRA 240

NLNSNVGVGK SAPNVYQDSI VVADFKSETE RLNLNSSKQP SNQRLKNGNK KTSIYADQKQ 300

SNNPVYKLIN TPGRKPERIV FNFNLIYPEN DEEFNTEEIL AMIKGLYKVQ RRGKKHTEDY 360

TSDKNRKKRK LDVLVERRQD LPSSQPPVVP KSTRIEVFKD DDNPSQSTHH KNTQVQVQTT 420

TSILPLKPVV DGNLAHETPV KPSLTSNASR SPTVTAFSKD AINEVFSMFN QHYSTPGALL 480

DGDDTTTSKF NVFENFTQEF TAKNIEDLTE VKDPKQETVS QQTTSTNETN DRYERLSNSS 540

TRPEKADYMT PIKETTETDV VPIIQTPKEQ IRTEDKKSGD NTETQTQLTS TTIQSSPFLT 600

QPEPQAEKLL QTAEHSEKSK EHYPTIIPPF TKIKNQPPVI IENPLSNNLR AKFLSEISPP 660

LFQYNTFYNY NQELKMSSLL KKIHRVSRNE NKNPIVDFKK TGDLYCIRGE LGEGGYATVY 720

LAESSQGHLR ALKVEKPASV WEYYIMSQVE FRLRKSTILK SIINASALHL FLDESYLVLN 780

YASQGTVLDL INLQREKAID GNGIMDEYLC MFITVELMKV LEKIHEVGII HGDLKPDNCM 840

IRLEKPGEPL GAHYMRNGED GWENKGIYLI DFGRSFDMTL LPPGTKFKSN WKADQQDCWE 900

MRAGKPWSYE ADYYGLAGVI HSMLFGKFIE TIQLQNGRCK LKNPFKRYWK KEIWGVIFDL 960

LLNSGQASNQ ALPMTEKIVE IRNLIESHLE QHAENHLRNV ILSIEEELSH FQYKGKPSRR 1020

F 1021

Nucleotide Sequence
(FASTA)

ATGAATTTAG ATTTAGGTTC TACTGTACGA GGCTATGAAT CAGATAAAGA CACATTTCCG 60

CAATCGAAAG GCGTTAGCTC ATCTCAAAAA GAGCAGCATA GTCAACTGAA TCAGACAAAA 120

ATAGCTTATG AACAGAGACT TCTAAATGAT TTAGAGGATA TGGATGATCC TTTAGATTTG 180

TTTTTAGATT ATATGATATG GATTAGCACC AGCTATATTG AAGTAGATTC TGAATCAGGT 240

CAAGAAGTTC TCCGAAGTAC AATGGAAAGA TGCTTGATTT ACATACAAGA TATGGAAACC 300

TATAGGAACG ATCCCAGATT TTTAAAAATT TGGATTTGGT ATATAAATTT GTTTCTTAGC 360

AACAACTTCC ACGAGAGTGA AAATACTTTT AAATATATGT TTAACAAGGG AATCGGAACA 420

AAGCTTTCGT TATTTTATGA AGAGTTCTCC AAGCTACTAG AAAATGCTCA ATTTTTTCTA 480

GAAGCAAAGG TGTTATTAGA ACTAGGGGCT GAAAACAATT GCAGGCCTTA CAACAGACTA 540

TTAAGGTCAT TAAGCAATTA TGAAGACAGA CTCCGAGAAA TGAACATCGT AGAAAACCAG 600

AACAGTGTAC CTGATTCAAG GGAAAGATTG AAGGGAAGGT TAATTTATAG AACAGCACCA 660

TTTTTCATTC GTAAATTTTT AACCTCATCA TTGATGACTG ATGACAAGGA AAATCGTGCG 720

AACCTGAACT CTAATGTTGG AGTTGGTAAG TCTGCTCCTA ACGTATATCA AGATTCTATA 780

GTGGTTGCTG ACTTTAAGTC AGAAACAGAG AGGCTTAATT TGAATAGCTC TAAACAGCCC 840

TCTAACCAAC GCTTGAAGAA TGGAAATAAA AAAACTTCAA TATATGCAGA TCAAAAGCAA 900

TCTAACAATC CAGTCTACAA ACTAATCAAT ACTCCGGGAC GGAAACCTGA ACGGATAGTT 960

TTTAATTTTA ATCTAATATA TCCAGAAAAT GATGAGGAGT TCAACACCGA AGAAATATTG 1020

GCAATGATTA AGGGACTTTA CAAGGTACAG CGCAGAGGTA AGAAGCACAC CGAAGATTAT 1080

ACAAGTGACA AAAATAGGAA GAAACGTAAG CTCGATGTGC TGGTAGAGAG ACGGCAAGAC 1140

TTACCTTCAT CACAGCCACC CGTTGTCCCC AAATCTACGC GAATTGAGGT GTTTAAAGAT 1200

GACGATAATC CCAGCCAGAG CACACACCAT AAAAATACAC AGGTACAAGT GCAAACGACA 1260

ACGTCTATTT TACCTTTGAA GCCTGTGGTT GATGGAAACC TCGCGCATGA GACACCAGTG 1320

AAGCCCTCAC TAACATCCAA CGCTTCCCGT TCACCTACAG TAACAGCTTT TTCAAAAGAC 1380

GCTATAAATG AGGTCTTTTC CATGTTCAAT CAACATTATT CAACCCCGGG TGCGCTATTA 1440

GATGGTGATG ATACCACTAC AAGTAAATTC AACGTCTTCG AAAATTTTAC CCAAGAATTT 1500

ACAGCAAAGA ATATTGAAGA TTTAACTGAA GTTAAAGATC CTAAACAAGA GACGGTGTCA 1560

CAACAAACTA CCTCAACAAA TGAAACCAAC GATCGATATG AAAGATTGTC GAATAGTAGC 1620

ACACGGCCAG AAAAGGCAGA CTACATGACG CCTATAAAAG AAACTACTGA AACAGATGTG 1680

GTACCTATAA TCCAGACACC TAAGGAACAA ATAAGGACAG AAGATAAGAA ATCGGGGGAT 1740

AATACAGAAA CACAAACTCA ATTAACAAGC ACAACCATTC AAAGTTCTCC ATTTCTCACA 1800

CAACCTGAAC CACAAGCAGA AAAGCTTTTA CAAACTGCAG AACATTCGGA AAAGAGTAAA 1860

GAGCATTACC CAACGATCAT ACCTCCCTTC ACTAAAATAA AAAATCAACC GCCTGTCATT 1920

ATTGAAAACC CACTTAGTAA TAATTTAAGA GCGAAGTTTT TATCCGAAAT TTCGCCCCCA 1980

TTATTCCAAT ATAACACCTT TTATAACTAC AACCAAGAAT TGAAAATGAG TTCTCTATTG 2040

AAAAAAATCC ATAGGGTATC CCGCAACGAA AACAAGAACC CAATTGTTGA CTTCAAGAAG 2100

ACGGGTGATT TATATTGTAT ACGCGGAGAA TTAGGCGAAG GAGGTTATGC CACTGTATAT 2160

CTAGCAGAAT CTAGTCAAGG ACATCTGCGA GCGTTGAAAG TAGAAAAACC TGCAAGTGTA 2220

TGGGAATATT ACATAATGAG CCAAGTGGAG TTTAGGTTGA GGAAGAGCAC AATATTAAAG 2280

TCAATAATCA ACGCTAGTGC TTTACACTTG TTCCTTGATG AGAGCTACCT TGTTTTAAAC 2340

TACGCCAGTC AAGGTACGGT CTTGGATTTA ATTAATTTAC AACGGGAAAA GGCAATTGAC 2400

GGCAACGGAA TAATGGATGA ATATTTGTGC ATGTTTATCA CTGTCGAGCT AATGAAAGTA 2460

CTTGAAAAGA TACATGAAGT GGGAATAATA CATGGCGATT TGAAACCAGA TAACTGCATG 2520

ATCAGATTAG AAAAACCAGG CGAACCCCTG GGTGCTCATT ATATGCGCAA TGGAGAGGAT 2580

GGCTGGGAAA ACAAGGGAAT CTATTTAATA GATTTCGGTA GATCATTTGA TATGACACTG 2640

TTACCACCTG GAACGAAATT CAAATCAAAT TGGAAGGCTG ATCAGCAAGA TTGCTGGGAA 2700

ATGCGTGCGG GCAAGCCATG GAGTTATGAA GCGGATTATT ATGGATTAGC AGGCGTCATA 2760

CATTCGATGC TTTTTGGAAA ATTCATAGAA ACAATCCAGC TGCAGAATGG ACGATGCAAA 2820

TTGAAGAACC CATTCAAAAG GTATTGGAAA AAAGAAATAT GGGGCGTTAT ATTTGATTTA 2880

CTGTTAAATA GCGGTCAAGC TTCCAATCAA GCTTTGCCGA TGACTGAAAA GATTGTTGAA 2940

ATAAGGAATT TGATAGAAAG CCATCTGGAA CAGCACGCAG AAAATCACTT AAGAAACGTA 3000

ATTCTAAGCA TTGAAGAGGA GTTATCACAT TTTCAATATA AGGGGAAACC GTCAAGGAGA 3060

TTTTAG 3066

Domain Profile

S: 1     leiekeigeGayakvykaehdsksgelvalKvekpansWefYIclqlkeRlkeeellksi  60

         ++i++e+geG+ya+vy ae  s++g+l+alKvekpa++We+YI++q+++Rl+++++lksi

Q: 705   YCIRGELGEGGYATVYLAE--SSQGHLRALKVEKPASVWEYYIMSQVEFRLRKSTILKSI  762

         79*****************..9**************************************

S: 61    ieissallfqdasllvleyasygtlldlinkvkevt...nkvmdeelvvllivqllkive  117

         i++s+++lf d+s+lvl+yas+gt+ldlin+++e++   n++mde+l+++++v+l+k++e

Q: 763   INASALHLFLDESYLVLNYASQGTVLDLINLQREKAidgNGIMDEYLCMFITVELMKVLE  822

         ************************************999*********************

S: 118   klHeveIiHgDlkPdnfl.........lgnrimdkadkdkeekglkliDfgrsiDmkllp  168

         k+Hev+IiHgDlkPdn++         lg+++m++ ++++e+kg++liDfgrs+Dm+llp

Q: 823   KIHEVGIIHGDLKPDNCMirlekpgepLGAHYMRNGEDGWENKGIYLIDFGRSFDMTLLP  882

         ************************************************************

S: 169   dgtkfkgkaktdafeciemlagkswsYqvDlfglaavihvllfgkyleviq.eggswklk  227

         +gtkfk+++k+d+++c+em+agk+wsY++D++gla+vih++lfgk++e+iq ++g++klk

Q: 883   PGTKFKSNWKADQQDCWEMRAGKPWSYEADYYGLAGVIHSMLFGKFIETIQlQNGRCKLK  942

         ************************************************************

S: 228   qkikrllkkelwekiferlLNisdast..lpsleelve  263

         +++kr++kke+w++if+ lLN+++as+  lp++e++ve

Q: 943   NPFKRYWKKEIWGVIFDLLLNSGQASNqaLPMTEKIVE  980

         *************************9999******997

Domain Sequence
(FASTA)

YCIRGELGEG GYATVYLAES SQGHLRALKV EKPASVWEYY IMSQVEFRLR KSTILKSIIN 60

ASALHLFLDE SYLVLNYASQ GTVLDLINLQ REKAIDGNGI MDEYLCMFIT VELMKVLEKI 120

HEVGIIHGDL KPDNCMIRLE KPGEPLGAHY MRNGEDGWEN KGIYLIDFGR SFDMTLLPPG 180

TKFKSNWKAD QQDCWEMRAG KPWSYEADYY GLAGVIHSML FGKFIETIQL QNGRCKLKNP 240

FKRYWKKEIW GVIFDLLLNS GQASNQALPM TEKIVE 276

Keyword

3D-structure; ATP-binding; Cell cycle; Complete proteome; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0000778	; C:condensed nuclear chromosome kinetochore
GO:0005524	; F:ATP binding
GO:0004672	; F:protein kinase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007094	; P:mitotic cell cycle spindle assembly checkpoint
GO:0034501	; P:protein localization to kinetochore
GO:0031134	; P:sister chromatid biorientation

KEGG

sce:YGR188C

;

InterPros

IPR011009	; Kinase-like_dom.
IPR012572	; Mad3-like.
IPR013212	; Mad3_BUB1_I.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.
IPR015661	; Ser/Thr_kinase_Bub1.

Pfam

PF08311	; Mad3_BUB1_I; 1.
PF08171	; Mad3_BUB1_II; 1.
PF00069	; Pkinase; 1.

SMARTs

SM00777

; Mad3_BUB1_I; 1.

Prosites

PS51489	; BUB1_N; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013