EKS-SAC-00102

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00102

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/MAPK	394.2	1.0E-118	23	318	296

Status

Reviewed

Ensembl Protein

YHR030C

UniProt Accession

Q00772; D3DKX7;

Protein Name

Mitogen-activated protein kinase SLT2/MPK1

Protein Synonyms/Alias

MAP kinase MPK1;

Gene Name

SLT2

Gene Synonyms/Alias

SLT2; MPK1; YHR030C;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YHR030C	YHR030C	YHR030C

Organism

Saccharomyces cerevisiae

Functional Description

Serine/threonine protein kinase involved in a signaltransduction pathway that play a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the SLT2/MPK1 kinase which itself then phosphorylates and activates the transcription factor RLM1. Directly phosphorylates BCY1 upon TOR complex 1 (TORC1) inhibition.

Protein Length

484

Protein Sequence
(FASTA)

MADKIERHTF KVFNQDFSVD KRFQLIKEIG HGAYGIVCSA RFAEAAEDTT VAIKKVTNVF 60

SKTLLCKRSL RELKLLRHFR GHKNITCLYD MDIVFYPDGS INGLYLYEEL MECDMHQIIK 120

SGQPLTDAHY QSFTYQILCG LKYIHSADVL HRDLKPGNLL VNADCQLKIC DFGLARGYSE 180

NPVENSQFLT EYVATRWYRA PEIMLSYQGY TKAIDVWSAG CILAEFLGGK PIFKGKDYVN 240

QLNQILQVLG TPPDETLRRI GSKNVQDYIH QLGFIPKVPF VNLYPNANSQ ALDLLEQMLA 300

FDPQKRITVD EALEHPYLSI WHDPADEPVC SEKFEFSFES VNDMEDLKQM VIQEVQDFRL 360

FVRQPLLEEQ RQLQLQQQQQ QQQQQQQQQQ QPSDVDNGNA AASEENYPKQ MATSNSVAPQ 420

QESFGIHSQN LPRHDADFPP RPQESMMEMR PATGNTADIP PQNDNGTLLD LEKELEFGLD 480

RKYF 484

Nucleotide Sequence
(FASTA)

ATGGCTGATA AGATAGAGAG GCATACTTTC AAGGTCTTCA ATCAAGATTT CAGTGTAGAT 60

AAGAGGTTTC AACTTATCAA AGAAATAGGG CATGGAGCAT ACGGCATAGT GTGTTCAGCG 120

CGGTTTGCAG AAGCTGCCGA AGATACCACA GTTGCCATCA AGAAAGTGAC AAACGTTTTT 180

TCGAAGACCT TACTATGTAA AAGATCCCTA CGTGAGCTAA AGCTTTTGAG ACATTTCAGA 240

GGCCACAAAA ATATTACATG TCTTTATGAT ATGGATATTG TTTTTTATCC AGACGGGTCT 300

ATCAATGGAC TATATCTTTA TGAGGAACTT ATGGAATGTG ATATGCACCA AATCATCAAA 360

TCCGGTCAAC CTTTGACGGA TGCTCACTAT CAAAGTTTCA CATACCAAAT ATTATGTGGT 420

TTAAAGTATA TTCATTCTGC AGATGTCTTG CATCGTGATT TGAAGCCCGG CAATTTGCTT 480

GTCAATGCAG ATTGTCAATT GAAAATCTGT GATTTTGGGT TAGCTAGAGG TTATTCGGAG 540

AATCCTGTCG AAAACAGTCA ATTTTTGACG GAGTACGTGG CCACTAGATG GTATAGAGCT 600

CCGGAAATAA TGTTGAGTTA CCAAGGATAT ACCAAGGCGA TTGACGTATG GTCAGCTGGC 660

TGTATTTTAG CGGAGTTTCT TGGTGGAAAG CCAATCTTCA AAGGAAAGGA TTACGTTAAT 720

CAATTGAATC AAATATTACA AGTTTTAGGG ACACCCCCAG ACGAAACTTT AAGAAGGATT 780

GGTTCTAAAA ATGTTCAGGA CTACATACAT CAATTAGGTT TCATTCCAAA AGTACCTTTT 840

GTCAATTTAT ACCCAAATGC CAATTCACAA GCATTAGACT TATTGGAGCA AATGCTCGCG 900

TTTGACCCTC AAAAGAGAAT TACCGTGGAT GAGGCCCTGG AGCATCCTTA CTTGTCTATA 960

TGGCATGATC CAGCTGACGA ACCTGTGTGT AGTGAAAAAT TCGAATTTAG TTTTGAATCG 1020

GTTAATGATA TGGAGGACTT AAAACAAATG GTTATACAAG AAGTGCAAGA TTTCAGGCTG 1080

TTTGTGAGAC AACCGCTATT AGAAGAGCAA AGGCAATTAC AATTACAGCA GCAGCAACAG 1140

CAGCAGCAAC AGCAACAGCA ACAGCAACAG CAGCCTTCAG ATGTGGATAA TGGCAACGCC 1200

GCAGCGAGTG AAGAAAATTA TCCAAAACAG ATGGCCACGT CTAATTCTGT TGCGCCACAA 1260

CAAGAATCAT TTGGTATTCA CTCCCAAAAT TTGCCAAGGC ATGATGCAGA TTTCCCACCT 1320

CGACCTCAAG AGAGTATGAT GGAGATGAGA CCTGCCACTG GAAATACCGC AGATATTCCG 1380

CCTCAGAATG ATAACGGCAC GCTTCTAGAC CTTGAAAAAG AGCTGGAGTT TGGATTAGAT 1440

AGAAAATATT TTTAG 1455

Domain Profile

S: 1     yeslkplgeGaygvvvsavdkrtee..rvaikklsrpfqketsakrtlRElkllkel.kh  57

         ++ +k++g+Gayg+v+sa  +++ e  +vaikk++++f+k+  +kr+lRElkll+++  h

Q: 23    FQLIKEIGHGAYGIVCSARFAEAAEdtTVAIKKVTNVFSKTLLCKRSLRELKLLRHFrGH  82

         57889*************9877655459*****************************99*

S: 58    eNiiklld...vftpeeeleelkdvYlvtelmetdLkkviksq.klsdehiklllyqilr  113

         +Ni +l+d   vf p+    +++ +Yl++elme+d++++iks   l+d h++++ yqil+

Q: 83    KNITCLYDmdiVFYPDG---SINGLYLYEELMECDMHQIIKSGqPLTDAHYQSFTYQILC  139

         *******9888899998...9*********************989***************

S: 114   glkylHsanviHrDlKPsNllvnedcelkildFGlarsadkekekk...lteyvatrwYr  170

         glky+Hsa+v+HrDlKP+Nllvn+dc+lki+dFGlar  +++  ++   lteyvatrwYr

Q: 140   GLKYIHSADVLHRDLKPGNLLVNADCQLKICDFGLARGYSENPVENsqfLTEYVATRWYR  199

         *************************************966665555579***********

S: 171   aPeillslkeytkavDiWsvGCIlaElltgkplfpgkdeidqlekilevlgtpseeflkk  230

         aPei+ls++ ytka+D+Ws+GCIlaE+l gkp+f+gkd+++ql++il+vlgtp +e+l++

Q: 200   APEIMLSYQGYTKAIDVWSAGCILAEFLGGKPIFKGKDYVNQLNQILQVLGTPPDETLRR  259

         ************************************************************

S: 231   ieseearnyikslpkkkkkdfeelfpkaseealdLleklLvldpdkRisveeaLehpYl  289

         i s+++++yi++l  ++k +f +l+p+a+++aldLle++L +dp+kRi+v+eaLehpYl

Q: 260   IGSKNVQDYIHQLGFIPKVPFVNLYPNANSQALDLLEQMLAFDPQKRITVDEALEHPYL  318

         **********************************************************7

Domain Sequence
(FASTA)

FQLIKEIGHG AYGIVCSARF AEAAEDTTVA IKKVTNVFSK TLLCKRSLRE LKLLRHFRGH 60

KNITCLYDMD IVFYPDGSIN GLYLYEELME CDMHQIIKSG QPLTDAHYQS FTYQILCGLK 120

YIHSADVLHR DLKPGNLLVN ADCQLKICDF GLARGYSENP VENSQFLTEY VATRWYRAPE 180

IMLSYQGYTK AIDVWSAGCI LAEFLGGKPI FKGKDYVNQL NQILQVLGTP PDETLRRIGS 240

KNVQDYIHQL GFIPKVPFVN LYPNANSQAL DLLEQMLAFD PQKRITVDEA LEHPYL 296

Keyword

ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Anolis carolinensis"; ?>Bos taurus"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Danio rerio"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pelodiscus sinensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005934	; C:cellular bud tip
GO:0005737	; C:cytoplasm
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0004707	; F:MAP kinase activity
GO:0000917	; P:barrier septum assembly
GO:0009272	; P:fungal-type cell wall biogenesis
GO:0030242	; P:peroxisome degradation
GO:0008361	; P:regulation of cell size
GO:0042990	; P:regulation of transcription factor import into nucleus
GO:0001101	; P:response to acid
GO:0030969	; P:UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response

KEGG

sce:YHR030C

;

InterPros

IPR011009	; Kinase-like_dom.
IPR003527	; MAP_kinase_CS.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS01351	; MAPK; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013