EKS-SAC-00011

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00011

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
STE/STE7	340.1	2.8E-102	360	623	264

Status

Reviewed

Ensembl Protein

YJL128C

UniProt Accession

P08018; D6VW57;

Protein Name

MAP kinase kinase PBS2

Protein Synonyms/Alias

Polymyxin B resistance protein 2; Suppressor of fluoride sensitivity 4;

Gene Name

PBS2

Gene Synonyms/Alias

PBS2; HOG4, SFS4, SSK4; YJL128C; J0699;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YJL128C	YJL128C	YJL128C

Organism

Saccharomyces cerevisiae

Functional Description

Kinase involved in a signal transduction pathway that isactivated by changes in the osmolarity of the extracellular environment. Seems to phosphorylate HOG1 on a tyrosine residue.

Protein Length

668

Protein Sequence
(FASTA)

MEDKFANLSL HEKTGKSSIQ LNEQTGSDNG SAVKRTSSTS SHYNNINADL HARVKAFQEQ 60

RALKRSASVG SNQSEQDKGS SQSPKHIQQI VNKPLPPLPV AGSSKVSQRM SSQVVQASSK 120

STLKNVLDNQ ETQNITDVNI NIDTTKITAT TIGVNTGLPA TDITPSVSNT ASATHKAQLL 180

NPNRRAPRRP LSTQHPTRPN VAPHKAPAII NTPKQSLSAR RGLKLPPGGM SLKMPTKTAQ 240

QPQQFAPSPS NKKHIETLSN SKVVEGKRSN PGSLINGVQS TSTSSSTEGP HDTVGTTPRT 300

GNSNNSSNSG SSGGGGLFAN FSKYVDIKSG SLNFAGKLSL SSKGIDFSNG SSSRITLDEL 360

EFLDELGHGN YGNVSKVLHK PTNVIMATKE VRLELDEAKF RQILMELEVL HKCNSPYIVD 420

FYGAFFIEGA VYMCMEYMDG GSLDKIYDES SEIGGIDEPQ LAFIANAVIH GLKELKEQHN 480

IIHRDVKPTN ILCSANQGTV KLCDFGVSGN LVASLAKTNI GCQSYMAPER IKSLNPDRAT 540

YTVQSDIWSL GLSILEMALG RYPYPPETYD NIFSQLSAIV DGPPPRLPSD KFSSDAQDFV 600

SLCLQKIPER RPTYAALTEH PWLVKYRNQD VHMSEYITER LERRNKILRE RGENGLSKNV 660

PALHMGGL 668

Nucleotide Sequence
(FASTA)

ATGGAAGACA AGTTTGCTAA CCTCAGTCTC CATGAGAAAA CTGGTAAGTC ATCTATCCAA 60

TTAAACGAGC AAACAGGCTC AGATAATGGC TCTGCTGTCA AGAGAACATC TTCGACGTCC 120

TCGCACTACA ATAACATCAA CGCTGACCTT CATGCTCGTG TAAAAGCTTT TCAAGAACAA 180

CGTGCATTGA AAAGGTCTGC CAGCGTGGGC AGTAATCAAA GCGAGCAAGA CAAAGGCAGT 240

TCACAATCAC CTAAACATAT TCAGCAGATT GTTAATAAGC CATTGCCGCC TCTTCCCGTA 300

GCAGGAAGTT CTAAGGTTTC ACAAAGAATG AGTAGCCAAG TCGTGCAAGC GTCCTCCAAG 360

AGCACTCTTA AGAACGTTCT GGACAATCAA GAAACACAAA ACATTACCGA CGTAAATATT 420

AACATCGATA CAACCAAAAT TACCGCCACA ACAATTGGTG TAAATACTGG CCTACCTGCT 480

ACTGACATTA CGCCGTCAGT TTCTAATACT GCATCAGCAA CACATAAGGC GCAATTGCTG 540

AATCCTAACA GAAGGGCACC AAGAAGGCCG CTTTCTACCC AGCACCCTAC AAGACCAAAT 600

GTTGCCCCGC ATAAGGCCCC TGCTATAATC AACACACCAA AACAAAGTTT AAGTGCCCGT 660

CGAGGGCTCA AATTACCACC AGGAGGAATG TCATTAAAAA TGCCCACTAA AACAGCTCAA 720

CAGCCGCAGC AGTTTGCCCC AAGCCCTTCA AACAAAAAAC ATATAGAAAC CTTATCAAAC 780

AGCAAAGTTG TTGAAGGGAA AAGATCGAAT CCGGGTTCTT TGATAAATGG TGTGCAAAGC 840

ACATCCACCT CATCAAGTAC CGAAGGCCCA CATGACACTG TAGGCACTAC ACCCAGAACT 900

GGAAACAGCA ACAACTCTTC AAATTCTGGT AGTAGTGGTG GTGGTGGTCT TTTCGCAAAT 960

TTCTCGAAAT ACGTGGATAT CAAATCCGGC TCTTTGAATT TTGCAGGCAA ACTATCGCTA 1020

TCCTCTAAAG GAATAGATTT CAGCAATGGT TCTAGTTCGA GAATTACATT GGACGAACTA 1080

GAATTTTTGG ATGAACTGGG TCATGGTAAC TATGGTAACG TCTCAAAGGT ACTGCATAAG 1140

CCCACAAATG TTATTATGGC GACGAAGGAA GTCCGTTTGG AGCTAGATGA GGCTAAATTT 1200

AGACAAATTT TAATGGAACT AGAAGTTTTG CATAAATGCA ATTCTCCCTA TATTGTGGAT 1260

TTTTATGGTG CATTCTTTAT TGAGGGCGCC GTCTACATGT GTATGGAATA CATGGATGGT 1320

GGTTCCTTGG ATAAAATATA CGACGAATCA TCTGAAATCG GCGGCATTGA TGAACCTCAG 1380

CTAGCGTTTA TTGCCAATGC TGTCATTCAT GGACTAAAAG AACTCAAAGA GCAGCATAAT 1440

ATCATACACA GAGATGTCAA ACCAACAAAT ATTTTATGTT CAGCCAACCA AGGCACCGTA 1500

AAGCTGTGCG ATTTCGGTGT TTCTGGTAAT TTGGTGGCAT CTTTAGCGAA GACTAATATT 1560

GGTTGTCAGT CATACATGGC ACCTGAACGA ATCAAATCGT TGAATCCAGA TAGAGCCACC 1620

TATACCGTAC AGTCAGACAT CTGGTCTTTA GGTTTAAGCA TTCTGGAAAT GGCACTAGGT 1680

AGATATCCGT ATCCACCAGA AACATACGAC AACATTTTCT CTCAATTGAG CGCTATTGTT 1740

GATGGGCCGC CACCGAGATT ACCTTCAGAT AAATTCAGTT CTGACGCACA AGATTTTGTT 1800

TCTTTATGTC TACAAAAGAT TCCGGAAAGA AGACCTACAT ACGCAGCTTT AACAGAGCAT 1860

CCTTGGTTAG TAAAATACAG AAACCAGGAT GTCCACATGA GTGAGTATAT CACTGAACGA 1920

TTAGAAAGGC GCAACAAAAT CTTACGGGAA CGTGGTGAGA ATGGTTTATC TAAAAATGTA 1980

CCGGCATTAC ATATGGGTGG TTTATAG 2007

Domain Profile

S: 1     lekleelGaGnggvVekvlhkksgkilavKtirleikkeeqkqilrelevlkkssnspyi  60

         le l+elG+Gn+g+V+kvlhk++++i+a+K++rle ++++ +qil+elevl+k+ nspyi

Q: 360   LEFLDELGHGNYGNVSKVLHKPTNVIMATKEVRLELDEAKFRQILMELEVLHKC-NSPYI  418

         67789*************************************************.*****

S: 61    vkfyGafvsegdvsicmelmdgtsldkvlkkvlekkkripekvlgkiavavvkaleylke  120

         v+fyGaf+ eg v++cme+mdg+sldk++ ++ ++ + i+e  l+ ia av+++l+ lke

Q: 419   VDFYGAFFIEGAVYMCMEYMDGGSLDKIYDES-SEIGGIDEPQLAFIANAVIHGLKELKE  477

         ******************************95.66899**********************

S: 121   klkiiHRDvKPsNiLvn.skGevKlCDFGvsgqlvdsiAksavGtksYmaPErieg....  175

         +++iiHRDvKP+NiL+  ++G+vKlCDFGvsg+lv s+Ak+++G++sYmaPEri++

Q: 478   QHNIIHRDVKPTNILCSaNQGTVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIKSlnpd  537

         ****************8579***********************************99999

S: 176   kkkYsvksdvWslGltlvelatgrypyeklke..ilellkaivneeppklpeeefseefq  233

         +++Y+v+sd+WslGl+++e+a grypy++++   i+++l+aiv+++pp+lp+++fs++ q

Q: 538   RATYTVQSDIWSLGLSILEMALGRYPYPPETYdnIFSQLSAIVDGPPPRLPSDKFSSDAQ  597

         99*************************9765444**************************

S: 234   efvskClkkdekeRpklkellkhafi  259

         +fvs Cl+k +++Rp++  l++h+++

Q: 598   DFVSLCLQKIPERRPTYAALTEHPWL  623

         ************************96

Domain Sequence
(FASTA)

LEFLDELGHG NYGNVSKVLH KPTNVIMATK EVRLELDEAK FRQILMELEV LHKCNSPYIV 60

DFYGAFFIEG AVYMCMEYMD GGSLDKIYDE SSEIGGIDEP QLAFIANAVI HGLKELKEQH 120

NIIHRDVKPT NILCSANQGT VKLCDFGVSG NLVASLAKTN IGCQSYMAPE RIKSLNPDRA 180

TYTVQSDIWS LGLSILEMAL GRYPYPPETY DNIFSQLSAI VDGPPPRLPS DKFSSDAQDF 240

VSLCLQKIPE RRPTYAALTE HPWL 264

Keyword

3D-structure; Antibiotic resistance; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005935	; C:cellular bud neck
GO:0005934	; C:cellular bud tip
GO:0031416	; C:NatB complex
GO:0005524	; F:ATP binding
GO:0004708	; F:MAP kinase kinase activity
GO:0004596	; F:peptide alpha-N-acetyltransferase activity
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0007015	; P:actin filament organization
GO:0000169	; P:activation of MAPK activity involved in osmosensory signaling pathway
GO:0034605	; P:cellular response to heat
GO:0006972	; P:hyperosmotic response
GO:0000208	; P:MAPK import into nucleus involved in osmosensory signaling pathway
GO:0017196	; P:N-terminal peptidyl-methionine acetylation
GO:0046677	; P:response to antibiotic

KEGG

sce:YJL128C

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013