EKS-SAC-00032

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00032

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Other/WEE	320.5	2.5E-96	444	709	266

Status

Reviewed

Ensembl Protein

YJL187C

UniProt Accession

P32944; D6VW03;

Protein Name

Mitosis inhibitor protein kinase SWE1

Protein Synonyms/Alias

Wee1 homolog;

Gene Name

SWE1

Gene Synonyms/Alias

SWE1; YJL187C; J0406;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YJL187C	YJL187C	YJL187C

Organism

Saccharomyces cerevisiae

Functional Description

Protein kinase that acts as a negative regulator ofentry into mitosis (G2 to M transition) by phosphorylating and inhibiting the mitosis-promoting cyclin B-bound CDC28 at 'Tyr-19'. SWE1-mediated inhibition of CDC28 acts in a cell size or morphogenesis checkpoint to delay mitosis in response to defects in growth, actin organization or bud formation. Inhibits the activity of B-type cyclins in replication initiation strongly for CLB2, moderately for CLB3 and CLB4, and there is no apparent inhibition for CLB5 and CLB6, correlating with the normal expression timing of those cyclins. Hyperphosphorylation and degradation of SWE1 when all checkpoint requirement are met releases CLB2-CDC28 from inhibition and allows for progression through the cell cycle. SWE1-dependent CDC28 phosphorylation is also required for pachytene arrest upon activation of the recombination checkpoint during meiosis. Also involved in the regulation of nitrogen starvation- and short chain alcohol-induced filamentous growth, or filamentous differentiation in response to slowed DNA synthesis. Can act both on serines and on tyrosines.

Protein Length

819

Protein Sequence
(FASTA)

MSSLDEDEED FEMLDTENLQ FMGKKMFGKQ AGEDESDDFA IGGSTPTNKL KFYPYSNNKL 60

TRSTGTLNLS LSNTALSEAN SKFLGKIEEE EEEEEEGKDE ESVDSRIKRW SPFHENESVT 120

TPITKRSAEK TNSPISLKQW NQRWFPKNDA RTENTSSSSS YSVAKPNQSA FTSSGLVSKM 180

SMDTSLYPAK LRIPETPVKK SPLVEGRDHK HVHLSSSKNA SSSLSVSPLN FVEDNNLQED 240

LLFSDSPSSK ALPSIHVPTI DSSPLSEAKY HAHDRHNNQT NILSPTNSLV TNSSPQTLHS 300

NKFKKIKRAR NSVILKNREL TNSLQQFKDD LYGTDENFPP PIIISSHHST RKNPQPYQFR 360

GRYDNDTDEE ISTPTRRKSI IGATSQTHRE SRPLSLSSAI VTNTTSAETH SISSTDSSPL 420

NSKRRLISSN KLSANPDSHL FEKFTNVHSI GKGQFSTVYQ VTFAQTNKKY AIKAIKPNKY 480

NSLKRILLEI KILNEVTNQI TMDQEGKEYI IDYISSWKFQ NSYYIMTELC ENGNLDGFLQ 540

EQVIAKKKRL EDWRIWKIIV ELSLALRFIH DSCHIVHLDL KPANVMITFE GNLKLGDFGM 600

ATHLPLEDKS FENEGDREYI APEIISDCTY DYKADIFSLG LMIVEIAANV VLPDNGNAWH 660

KLRSGDLSDA GRLSSTDIHS ESLFSDITKV DTNDLFDFER DNISGNSNNA GTSTVHNNSN 720

INNPNMNNGN DNNNVNTAAT KNRLILHKSS KIPAWVPKFL IDGESLERIV RWMIEPNYER 780

RPTANQILQT EECLYVEMTR NAGAIIQEDD FGPKPKFFI 819

Nucleotide Sequence
(FASTA)

ATGAGTTCTT TGGACGAGGA TGAAGAGGAC TTCGAAATGC TGGACACGGA GAACCTCCAG 60

TTTATGGGGA AGAAGATGTT TGGCAAACAG GCCGGCGAAG ACGAGAGTGA TGATTTTGCT 120

ATAGGGGGTA GCACCCCGAC CAATAAACTG AAATTTTATC CATATTCGAA CAACAAATTG 180

ACAAGAAGTA CGGGGACCTT GAACCTGTCA TTAAGTAATA CAGCTTTGTC AGAGGCTAAC 240

TCCAAATTTC TTGGGAAAAT TGAAGAAGAG GAAGAAGAGG AGGAAGAAGG CAAGGATGAG 300

GAAAGCGTGG ATTCTCGTAT TAAAAGGTGG TCTCCGTTCC ATGAAAATGA AAGTGTTACT 360

ACTCCTATTA CAAAAAGATC TGCGGAAAAA ACGAACAGTC CTATTTCTCT CAAACAATGG 420

AACCAGCGAT GGTTTCCGAA AAATGATGCT CGCACTGAAA ATACATCCTC ATCCTCTTCA 480

TATAGCGTCG CTAAACCTAA CCAATCAGCC TTTACGTCTT CGGGCCTCGT ATCTAAAATG 540

TCTATGGACA CTTCGTTATA CCCTGCGAAA TTGAGGATAC CAGAAACACC AGTGAAAAAA 600

TCACCCTTAG TGGAGGGAAG AGACCATAAG CATGTCCACC TTTCGAGTTC GAAAAATGCA 660

TCGTCTTCTC TAAGTGTTTC CCCTTTAAAT TTTGTTGAAG ACAATAATTT ACAAGAAGAC 720

CTTTTATTTT CAGATTCTCC GTCTTCGAAA GCTTTACCTT CCATCCATGT ACCAACCATA 780

GACTCATCCC CACTGAGCGA GGCAAAATAT CATGCACATG ATCGTCACAA TAACCAGACA 840

AACATCCTGT CTCCCACTAA TAGCTTGGTT ACCAACAGCT CTCCACAAAC ATTGCATTCT 900

AACAAGTTCA AAAAAATCAA AAGAGCAAGG AATTCGGTTA TTTTGAAAAA TAGAGAGCTA 960

ACAAACAGTT TACAACAATT CAAAGATGAT TTATACGGCA CGGACGAGAA TTTCCCACCT 1020

CCAATCATAA TATCAAGTCA TCATTCAACT AGAAAGAACC CTCAACCTTA TCAATTTCGT 1080

GGACGCTATG ACAATGACAC TGACGAAGAG ATCTCCACTC CAACAAGACG AAAATCTATT 1140

ATTGGGGCAA CATCTCAAAC ACATAGAGAA AGCAGACCAT TGTCACTCTC CTCTGCCATC 1200

GTGACAAACA CAACAAGTGC AGAGACGCAT TCCATATCTT CCACCGATTC TTCGCCGTTA 1260

AATTCCAAAA GGCGTCTAAT CTCTTCAAAT AAGTTATCAG CAAATCCAGA TTCCCATCTT 1320

TTCGAAAAAT TTACGAATGT GCATTCCATT GGTAAAGGCC AGTTTTCCAC GGTCTACCAG 1380

GTTACGTTTG CCCAAACAAA CAAAAAGTAT GCAATCAAAG CCATTAAACC AAACAAATAT 1440

AATTCCTTGA AACGCATATT ACTGGAAATT AAAATACTAA ACGAGGTAAC AAACCAAATT 1500

ACAATGGATC AAGAAGGGAA GGAATACATC ATCGATTACA TCAGTTCCTG GAAGTTTCAA 1560

AATTCATACT ATATTATGAC AGAATTGTGC GAAAATGGTA ATTTGGATGG ATTTTTACAA 1620

GAGCAAGTTA TCGCAAAGAA AAAAAGGTTG GAAGATTGGA GAATTTGGAA AATCATCGTG 1680

GAATTAAGCC TGGCTTTACG ATTCATCCAT GATTCTTGTC ACATTGTGCA TCTGGACTTG 1740

AAACCCGCAA ACGTCATGAT CACATTTGAA GGTAACCTAA AACTAGGTGA CTTTGGAATG 1800

GCTACTCATT TACCGTTGGA GGATAAAAGT TTTGAAAATG AAGGTGACAG AGAATATATT 1860

GCACCAGAAA TCATTTCTGA TTGTACGTAC GATTACAAGG CAGATATTTT TTCCCTGGGT 1920

CTGATGATTG TTGAAATTGC AGCGAACGTT GTGTTACCTG ACAATGGCAA CGCATGGCAT 1980

AAGTTGAGAT CGGGTGATTT ATCGGATGCA GGAAGATTAA GTTCCACAGA TATTCATTCT 2040

GAATCATTAT TTTCAGACAT TACGAAAGTA GATACAAATG ATTTATTTGA TTTTGAAAGA 2100

GACAATATCA GTGGTAATAG TAACAACGCT GGCACCTCCA CTGTTCATAA CAATAGTAAT 2160

ATCAACAACC CTAATATGAA TAATGGCAAC GATAATAATA ATGTCAATAC TGCCGCTACC 2220

AAGAATCGTC TTATTTTGCA TAAAAGTTCT AAAATTCCCG CATGGGTACC GAAATTTCTT 2280

ATTGATGGTG AATCACTTGA GAGAATAGTA CGATGGATGA TAGAGCCCAA TTATGAGAGA 2340

AGGCCCACGG CAAATCAAAT CTTACAAACT GAGGAATGCC TGTATGTAGA AATGACACGC 2400

AATGCAGGTG CTATTATCCA GGAAGACGAC TTTGGACCTA AGCCAAAATT TTTTATATGA 2460

Domain Profile

S: 1     FeevekiGkGefgevfkvvkredgklyAikksk....kplkglskekkalrEvtahavlg  56

         F++v++iGkG+f++v++v++++++k+yAik++k    ++lk++  e+k+l+Evt++++++

Q: 444   FTNVHSIGKGQFSTVYQVTFAQTNKKYAIKAIKpnkyNSLKRILLEIKILNEVTNQITMD  503

         99******************************999999**********************

S: 57    q..henvvryisaWeeddslyiqtElCeggsLeaalqe.ceakkkaleeaelkkilldll  113

         q  +e++++yis+W++++s+yi+tElCe+g+L+ +lqe ++akkk+le+++++ki+++l+

Q: 504   QegKEYIIDYISSWKFQNSYYIMTELCENGNLDGFLQEqVIAKKKRLEDWRIWKIIVELS  563

         *999**********************************9*********************

S: 114   lglrylHss.slvHlDikpenifitkevtlklgDlGlvtelnsedkskveEGDsrylakE  172

         l+lr++H+s ++vHlD+kp+n++it+e++lklgD+G++t+l++edks+++EGD++y+a+E

Q: 564   LALRFIHDScHIVHLDLKPANVMITFEGNLKLGDFGMATHLPLEDKSFENEGDREYIAPE  623

         *********9**************************************************

S: 173   ilqeeysklkaDiFslgltilevaanvelPsnGeaWhklRkgklpd..............  218

         i++++++++kaDiFslgl+i+e+aanv lP+nG+aWhklR+g+l+d

Q: 624   IISDCTYDYKADIFSLGLMIVEIAANVVLPDNGNAWHKLRSGDLSDagrlsstdihsesl  683

         ************************************************************

S: 219   ..eilkelsedlrellklmlepdper  242

           +i+k++++dl++  +  +  + ++

Q: 684   fsDITKVDTNDLFDFERDNISGNSNN  709

         ************99877777666655

Domain Sequence
(FASTA)

FTNVHSIGKG QFSTVYQVTF AQTNKKYAIK AIKPNKYNSL KRILLEIKIL NEVTNQITMD 60

QEGKEYIIDY ISSWKFQNSY YIMTELCENG NLDGFLQEQV IAKKKRLEDW RIWKIIVELS 120

LALRFIHDSC HIVHLDLKPA NVMITFEGNL KLGDFGMATH LPLEDKSFEN EGDREYIAPE 180

IISDCTYDYK ADIFSLGLMI VEIAANVVLP DNGNAWHKLR SGDLSDAGRL SSTDIHSESL 240

FSDITKVDTN DLFDFERDNI SGNSNN 266

Keyword

ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Magnesium; Meiosis; Metal-binding; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0005935	; C:cellular bud neck
GO:0005634	; C:nucleus
GO:0005524	; F:ATP binding
GO:0046872	; F:metal ion binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0004713	; F:protein tyrosine kinase activity
GO:0051301	; P:cell division
GO:0000078	; P:cell shape checkpoint
GO:0000086	; P:G2/M transition of mitotic cell cycle
GO:0007126	; P:meiosis
GO:0007067	; P:mitosis
GO:0010697	; P:negative regulation of spindle pole body separation
GO:0000320	; P:re-entry into mitotic cell cycle
GO:0008361	; P:regulation of cell size
GO:0000079	; P:regulation of cyclin-dependent protein kinase activity
GO:0040020	; P:regulation of meiosis

KEGG

sce:YJL187C

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013