EKS-SAC-00045

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00045

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
AGC/Akt	437.6	5.2E-132	347	602	256

Status

Reviewed

Ensembl Protein

YKL126W

UniProt Accession

P12688; D6VX69;

Protein Name

Serine/threonine-protein kinase YPK1

Protein Synonyms/Alias

Sphingosine-like immunosuppressant resistant protein 2; Yeast protein kinase 1;

Gene Name

YPK1

Gene Synonyms/Alias

YPK1; SLI2; YKL126W;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YKL126W	YKL126W	YKL126W

Organism

Saccharomyces cerevisiae

Functional Description

Plays an essential role in the proliferation of yeastcells. Involved in a signaling pathway, required for optimal cell wall integrity, that acts in parallel with the PKC1-SLT2-dependent pathway. Downstream kinase in the sphingolipid-mediated signaling pathway. Its phosphorylation is regulated by the intracellular sphingolipid concentration. Cooperates with SLI1 in mediating resistance to the sphingolipid biosynthesis inhibitor drug myriocin (ISP-1). Its kinase activity is essential for the resistance. Required for both receptor-mediated and fluid-phase endocytosis, but is not necessary for receptor phosphorylation or ubiquitination. Necessary for the internalization of plasma membrane proteins carrying different types of internalization signals. Acts downstream of the PKH kinases to control endocytosis by phosphorylating components of the endocytic machinery. Phosphorylation of residue Thr-504 in the activation loop is essential for activity. phosphorylates and down-regulates flippase activator FPK1.

Protein Length

680

Protein Sequence
(FASTA)

MYSWKSKFKF GKSKEEKEAK HSGFFHSSKK EEQQNNQATA GEHDASITRS SLDRKGTINP 60

SNSSVVPVRV SYDASSSTST VRDSNGGNSE NTNSSQNLDE TANIGSTGTP NDATSSSGMM 120

TIKVYNGDDF ILPFPITSSE QILNKLLASG VPPPHKEISK EVDALIAQLS RVQIKNQGPA 180

DEDLISSESA AKFIPSTIML PGSSTLNPLL YFTIEFDNTV ATIEAEYGTI AKPGFNKIST 240

FDVTRKLPYL KIDVFARIPS ILLPSKTWQQ EMGLQDEKLQ TIFDKINSNQ DIHLDSFHLP 300

INLSFDSAAS IRLYNHHWIT LDNGLGKINI SIDYKPSRNK PLSIDDFDLL KVIGKGSFGK 360

VMQVRKKDTQ KVYALKAIRK SYIVSKSEVT HTLAERTVLA RVDCPFIVPL KFSFQSPEKL 420

YFVLAFINGG ELFYHLQKEG RFDLSRARFY TAELLCALDN LHKLDVVYRD LKPENILLDY 480

QGHIALCDFG LCKLNMKDDD KTDTFCGTPE YLAPELLLGL GYTKAVDWWT LGVLLYEMLT 540

GLPPYYDEDV PKMYKKILQE PLVFPDGFDR DAKDLLIGLL SRDPTRRLGY NGADEIRNHP 600

FFSQLSWKRL LMKGYIPPYK PAVSNSMDTS NFDEEFTREK PIDSVVDEYL SESVQKQFGG 660

WTYVGNEQLG SSMVQGRSIR 680

Nucleotide Sequence
(FASTA)

ATGTATTCTT GGAAGTCAAA GTTTAAGTTT GGAAAATCTA AAGAAGAAAA AGAAGCCAAA 60

CATAGTGGGT TTTTTCACTC CTCTAAAAAG GAAGAACAGC AGAATAATCA AGCAACTGCG 120

GGGGAACATG ATGCTTCAAT TACTCGTTCA TCTTTAGACA GGAAGGGCAC GATAAATCCA 180

TCAAATTCAT CAGTGGTCCC GGTTCGTGTT TCATACGATG CATCCTCATC GACCTCTACT 240

GTACGAGATT CAAATGGTGG TAATTCAGAA AACACGAACT CATCTCAAAA TTTAGATGAA 300

ACAGCAAATA TTGGTTCTAC AGGGACACCC AATGATGCCA CCTCAAGTTC AGGAATGATG 360

ACTATCAAAG TATATAATGG TGATGACTTT ATTTTACCTT TTCCGATTAC CTCAAGCGAG 420

CAAATATTGA ATAAACTATT GGCCTCGGGT GTTCCACCGC CACATAAGGA GATTAGTAAA 480

GAAGTAGATG CTTTAATTGC ACAACTGTCC CGTGTTCAAA TAAAGAACCA AGGTCCAGCT 540

GATGAAGATT TGATCTCTAG CGAGTCAGCA GCAAAGTTCA TCCCATCTAC TATCATGTTA 600

CCAGGGTCTT CGACCTTAAA TCCCTTACTA TATTTCACCA TTGAATTTGA TAATACAGTT 660

GCAACTATTG AAGCAGAGTA TGGTACGATC GCTAAACCTG GTTTCAATAA GATATCTACC 720

TTTGATGTGA CAAGGAAATT ACCTTATTTA AAGATTGATG TATTTGCAAG AATTCCGTCC 780

ATTCTTTTGC CCTCGAAGAC ATGGCAACAG GAGATGGGTT TGCAAGACGA GAAGCTACAG 840

ACCATTTTTG ATAAAATAAA TTCCAATCAA GATATACATT TAGATTCTTT CCATTTGCCC 900

ATCAACTTAA GTTTTGACTC TGCAGCTAGT ATTAGACTAT ATAATCACCA CTGGATCACA 960

TTGGATAATG GCTTGGGTAA GATCAATATT AGTATTGATT ATAAACCTTC CAGAAATAAA 1020

CCTTTGTCCA TCGATGATTT CGATCTTTTG AAAGTTATCG GTAAGGGTTC GTTTGGTAAA 1080

GTGATGCAAG TCAGAAAGAA AGATACACAA AAAGTATACG CTTTGAAGGC AATCAGAAAA 1140

TCATACATTG TCTCTAAATC CGAAGTCACG CATACTTTGG CAGAAAGAAC CGTTCTAGCA 1200

CGTGTTGATT GTCCATTTAT TGTACCTTTG AAGTTTTCTT TCCAATCACC GGAAAAATTA 1260

TACTTTGTTT TAGCGTTTAT CAATGGTGGT GAGTTGTTTT ATCATCTACA AAAGGAAGGA 1320

AGGTTTGATT TATCACGTGC CAGATTTTAT ACCGCAGAAT TGTTATGTGC GCTAGACAAC 1380

TTGCATAAAC TAGATGTTGT CTATCGTGAT TTGAAACCAG AGAATATTTT ATTGGATTAT 1440

CAAGGCCACA TTGCCCTTTG TGATTTCGGG CTATGCAAAT TGAATATGAA GGATGATGAT 1500

AAGACAGATA CTTTTTGTGG GACCCCAGAA TACTTGGCAC CAGAACTATT GCTAGGTTTA 1560

GGCTATACAA AGGCAGTAGA TTGGTGGACA TTGGGAGTCT TGTTATACGA GATGCTCACA 1620

GGTCTTCCTC CTTATTATGA TGAAGATGTT CCAAAAATGT ATAAGAAGAT TCTACAGGAG 1680

CCACTAGTTT TCCCAGATGG ATTTGATAGA GATGCAAAGG ATCTATTGAT TGGATTATTG 1740

AGCCGTGATC CGACAAGAAG ATTGGGCTAC AATGGTGCCG ACGAAATTCG GAACCATCCT 1800

TTTTTCAGCC AATTATCATG GAAGCGCCTG TTGATGAAGG GTTATATTCC ACCATATAAA 1860

CCAGCTGTTA GTAATTCCAT GGATACTAGT AATTTCGATG AGGAATTCAC TAGGGAGAAG 1920

CCAATTGATA GTGTGGTAGA TGAATACTTG AGTGAAAGTG TTCAAAAGCA ATTTGGTGGC 1980

TGGACATACG TTGGAAATGA ACAGCTAGGT AGCTCAATGG TGCAAGGTAG AAGCATTAGA 2040

TAG 2043

Domain Profile

S: 1     fdllkllGkGtfGkvilvrekatqklyalkilkkevivakdevahtlterrvlkrtkhpf  60

         fdllk++GkG+fGkv++vr+k+tqk+yalk+++k++iv+k+ev+htl+er+vl+r+++pf

Q: 347   FDLLKVIGKGSFGKVMQVRKKDTQKVYALKAIRKSYIVSKSEVTHTLAERTVLARVDCPF  406

         9***********************************************************

S: 61    lvalkysfqtkeklclvleyvnGGelffhlskervfsedrarfygaeivsaldylhskdv  120

         +v lk+sfq++ekl++vl+++nGGelf+hl+ke++f+ +rarfy+ae+++ald+lh+ dv

Q: 407   IVPLKFSFQSPEKLYFVLAFINGGELFYHLQKEGRFDLSRARFYTAELLCALDNLHKLDV  466

         ************************************************************

S: 121   vyrdlklenllldkdGhikitdfGlckeeikdgdktktfcGtpeylapevlededygkav  180

         vyrdlk+en+lld++Ghi+++dfGlck ++kd+dkt+tfcGtpeylape+l + +y+kav

Q: 467   VYRDLKPENILLDYQGHIALCDFGLCKLNMKDDDKTDTFCGTPEYLAPELLLGLGYTKAV  526

         ************************************************************

S: 181   dwwglGvvlyemlcGrlpfynkdheklfelilleelkfprklseeaksllsGllkkdpkk  240

         dww+lGv+lyeml+G++p+y++d++k++++il+e+l fp+ ++++ak+ll+Gll++dp++

Q: 527   DWWTLGVLLYEMLTGLPPYYDEDVPKMYKKILQEPLVFPDGFDRDAKDLLIGLLSRDPTR  586

         ************************************************************

S: 241   rlGggkddakeireheff  258

         rlG  +++a+eir+h+ff

Q: 587   RLG--YNGADEIRNHPFF  602

         **9..8***********9

Domain Sequence
(FASTA)

FDLLKVIGKG SFGKVMQVRK KDTQKVYALK AIRKSYIVSK SEVTHTLAER TVLARVDCPF 60

IVPLKFSFQS PEKLYFVLAF INGGELFYHL QKEGRFDLSR ARFYTAELLC ALDNLHKLDV 120

VYRDLKPENI LLDYQGHIAL CDFGLCKLNM KDDDKTDTFC GTPEYLAPEL LLGLGYTKAV 180

DWWTLGVLLY EMLTGLPPYY DEDVPKMYKK ILQEPLVFPD GFDRDAKDLL IGLLSRDPTR 240

RLGYNGADEI RNHPFF 256

Keyword

ATP-binding; Cell membrane; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Endocytosis; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Choloepus hoffmanni"; ?>Danio rerio"; ?>Dasypus novemcinctus"; ?>Dipodomys ordii"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Microcebus murinus"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sorex araneus"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Tupaia belangeri"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>

Gene Ontology

GO:0005935	; C:cellular bud neck
GO:0005829	; C:cytosol
GO:0005886	; C:plasma membrane
GO:0005524	; F:ATP binding
GO:0004674	; F:protein serine/threonine kinase activity
GO:0007047	; P:cellular cell wall organization
GO:0070941	; P:eisosome assembly
GO:0006897	; P:endocytosis
GO:0061093	; P:negative regulation of phospholipid translocation
GO:0046777	; P:protein autophosphorylation

KEGG

sce:YKL126W

;

InterPros

IPR000961	; AGC-kinase_C.
IPR011009	; Kinase-like_dom.
IPR017892	; Pkinase_C.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069	; Pkinase; 1.
PF00433	; Pkinase_C; 1.

SMARTs

SM00133	; S_TK_X; 1.
SM00220	; S_TKc; 1.

Prosites

PS51285	; AGC_KINASE_CTER; 1.
PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013