EKS-SAC-00066

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00066

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	351.1	1.4E-105	183	469	287

Status

Reviewed

Ensembl Protein

YKL139W

UniProt Accession

Q03957; D6VX57;

Protein Name

CTD kinase subunit alpha

Protein Synonyms/Alias

CTDK-I subunit alpha; CTD kinase 58 kDa subunit; CTD kinase subunit 1;

Gene Name

CTK1

Gene Synonyms/Alias

CTK1; YKL139W;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YKL139W	YKL139W	YKL139W

Organism

Saccharomyces cerevisiae

Functional Description

Catalytic subunit of the CTDK-I complex, whichhyperphosphorylates the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit. CTDK-I phosphorylates 'Ser-5' if the CTD substrate is not phosphorylated at 'Ser-5', but will phosphorylate 'Ser-2' of a CTD substrate if 'Ser-5' is already phosphorylated. CTDK-I is also more reactive toward substrates that are prephosphorylated at 'Ser-2' or 'Ser-5' compared with an unphosphorylated CTD substrate, therefore efficiently creating doubly phosphorylated CTD repeats. Involved in RNA polymerase II transcriptional elongation, and through PTI1, pre-mRNA 3'-end processing. Participates in both positive and negative regulation of CTD phosphorylation. Required for DNA damage induced transcription, including the expression of the RNR genes, and reprogramming of gene expression upon amino acid starvation. Required for SET2 mediated H3K36 methylation. Also regulates H3K4 methylation. Controls the maintenance of suppressive chromatin in the coding regions of genes by both promoting H3K36 methylation, which leads to histone deacetylation, and catalyzing phosphorylation of the CTD required to localize H3K4 chromatin modification specifically to the 5' ends of genes, thereby creating a boundary for H3K4 methylation that prevents a mark associated with transcriptional initiation from spreading into the bodies of genes. Involved in RNA polymerase I transcription. Involved in telomere maintenance. Acts together with SNF1 to induce GSY2 transcription in response to glucose limitation. Involved in the adaptation to alternative carbon sources, including galactose, glycerol and ethanol, but not raffinose. Required for the integrity of the rDNA locus. Functions in translation elongation by enhancing decoding fidelity. Needed for translational accuracy by phosphorylating RPS2.

Protein Length

528

Protein Sequence
(FASTA)

MSYNNGNTYS KSYSRNNKRP LFGKRSPNPQ SLARPPPPKR IRTDSGYQSN MDNISSHRVN 60

SNDQPGHTKS RGNNNLSRYN DTSFQTSSRY QGSRYNNNNT SYENRPKSIK RDETKAEFLS 120

HLPKGPKSVE KSRYNNSSNT SNDIKNGYHA SKYYNHKGQE GRSVIAKKVP VSVLTQQRST 180

SVYLRIMQVG EGTYGKVYKA KNTNTEKLVA LKKLRLQGER EGFPITSIRE IKLLQSFDHP 240

NVSTIKEIMV ESQKTVYMIF EYADNDLSGL LLNKEVQISH SQCKHLFKQL LLGMEYLHDN 300

KILHRDVKGS NILIDNQGNL KITDFGLARK MNSRADYTNR VITLWYRPPE LLLGTTNYGT 360

EVDMWGCGCL LVELFNKTAI FQGSNELEQI ESIFKIMGTP TINSWPTLYD MPWFFMIMPQ 420

QTTKYVNNFS EKFKSVLPSS KCLQLAINLL CYDQTKRFSA TEALQSDYFK EEPKPEPLVL 480

DGLVSCHEYE VKLARKQKRP NILSTNTNNK GNGNSNNNNN NNNDDDDK 528

Nucleotide Sequence
(FASTA)

ATGTCCTACA ATAATGGCAA TACTTATTCA AAGAGTTATA GCAGAAATAA TAAGAGACCC 60

TTGTTCGGAA AGAGGTCGCC AAATCCTCAG TCCCTAGCGA GACCACCGCC ACCAAAGAGA 120

ATACGGACTG ATAGTGGTTA CCAGTCGAAT ATGGACAATA TATCTTCTCA TAGGGTAAAT 180

TCAAATGACC AACCAGGCCA CACGAAAAGT CGTGGTAACA ATAATTTATC TCGCTATAAC 240

GATACATCCT TTCAAACAAG TTCCAGATAT CAAGGTTCAA GATACAACAA TAATAATACG 300

TCGTATGAAA ATAGGCCTAA AAGCATAAAA AGGGATGAAA CAAAGGCTGA ATTCCTGTCT 360

CATTTGCCAA AGGGGCCCAA ATCTGTTGAG AAATCAAGAT ATAATAATTC ATCCAATACT 420

AGTAATGATA TAAAAAATGG CTATCATGCT TCAAAATACT ATAACCACAA AGGCCAGGAA 480

GGGCGGTCTG TCATAGCTAA GAAAGTTCCA GTTTCGGTTC TAACGCAGCA AAGGAGCACG 540

TCAGTTTACC TAAGGATAAT GCAAGTCGGA GAAGGAACAT ATGGTAAAGT TTACAAGGCA 600

AAAAACACGA ATACAGAGAA GTTAGTAGCG CTGAAAAAAT TGAGATTACA AGGAGAGAGG 660

GAAGGCTTTC CCATAACCTC TATACGAGAG ATTAAACTAC TACAAAGTTT TGATCATCCA 720

AATGTCTCTA CTATAAAAGA AATAATGGTC GAATCTCAAA AAACTGTATA TATGATATTT 780

GAATATGCTG ACAACGACTT AAGTGGATTA CTATTAAATA AGGAAGTCCA AATTTCTCAC 840

TCACAATGCA AACATCTCTT CAAACAATTG CTATTAGGAA TGGAATATTT ACATGATAAT 900

AAGATTTTAC ACCGTGATGT TAAAGGCTCA AACATCTTAA TCGATAACCA GGGGAATCTA 960

AAAATAACAG ATTTTGGACT AGCGAGGAAA ATGAATTCGC GAGCTGATTA CACTAACCGT 1020

GTCATTACGT TGTGGTACAG ACCACCAGAA CTATTGTTGG GAACTACAAA TTATGGAACA 1080

GAAGTTGACA TGTGGGGTTG TGGCTGCCTC CTGGTGGAAT TATTCAATAA AACTGCAATT 1140

TTCCAGGGCT CTAATGAATT AGAGCAAATA GAATCAATTT TCAAGATTAT GGGAACTCCT 1200

ACAATAAATA GCTGGCCAAC GCTTTACGAT ATGCCGTGGT TTTTTATGAT TATGCCACAG 1260

CAAACTACCA AGTATGTCAA CAATTTTTCT GAGAAATTTA AAAGCGTTTT ACCATCTTCA 1320

AAATGTCTGC AATTGGCGAT TAATTTGTTA TGTTATGATC AGACGAAAAG GTTTAGTGCA 1380

ACCGAAGCTT TACAAAGCGA CTATTTCAAG GAAGAACCAA AGCCTGAACC TTTAGTTCTT 1440

GATGGATTGG TAAGTTGCCA CGAATATGAA GTCAAATTGG CAAGAAAACA AAAGCGTCCT 1500

AACATACTAT CCACCAACAC AAACAATAAG GGTAATGGTA ATAGTAATAA TAATAATAAT 1560

AATAATAATG ACGATGATGA TAAATAA 1587

Domain Profile

S: 1     yeklekigeGtygkvykakdketgevvAlKkirlekekegvpitalrEisllkelkhkni  60

         y ++ ++geGtygkvykak+++t+++vAlKk+rl+ e+eg+pit++rEi+ll++++h+n+

Q: 183   YLRIMQVGEGTYGKVYKAKNTNTEKLVALKKLRLQGEREGFPITSIREIKLLQSFDHPNV  242

         567889******************************************************

S: 61    vkLlevvakdkkklyLvfefleqdLkkllkkkkkkklsleevkslllqlleglaylHsnk  120

          +++e++++++k++y++fe++++dL+ ll +k+  ++s++++k+l++qll g++ylH+nk

Q: 243   STIKEIMVESQKTVYMIFEYADNDLSGLLLNKEV-QISHSQCKHLFKQLLLGMEYLHDNK  301

         ********999**********************9.*************************

S: 121   ilHRDlKpqNlLiskegklklaDfGlarafssplktytkevvTlwYraPelLlgakeYst  180

         ilHRD+K +N+Li+++g+lk++DfGlar+++s+ + yt++v+TlwYr+PelLlg+++Y+t

Q: 302   ILHRDVKGSNILIDNQGNLKITDFGLARKMNSRAD-YTNRVITLWYRPPELLLGTTNYGT  360

         ******************************99888.************************

S: 181   avDiWsvgcilaelltrkplfqgkseidqlerifkllgtpsekvwpevsklpeykksfpk  240

         +vD+W++gc+l+el++++++fqg++e +q+e ifk++gtp+ ++wp++ ++p + + +p+

Q: 361   EVDMWGCGCLLVELFNKTAIFQGSNELEQIESIFKIMGTPTINSWPTLYDMPWFFMIMPQ  420

         ******************************************************977777

S: 241   kkkk...klkekvkklk..ekaldllekllaldpkkRisakealqhkyf  284

         +++k   +++ek+k++   +k+l+l  +ll++d++kR+sa+ealq++yf

Q: 421   QTTKyvnNFSEKFKSVLpsSKCLQLAINLLCYDQTKRFSATEALQSDYF  469

         6554235777888777664669**************************9

Domain Sequence
(FASTA)

YLRIMQVGEG TYGKVYKAKN TNTEKLVALK KLRLQGEREG FPITSIREIK LLQSFDHPNV 60

STIKEIMVES QKTVYMIFEY ADNDLSGLLL NKEVQISHSQ CKHLFKQLLL GMEYLHDNKI 120

LHRDVKGSNI LIDNQGNLKI TDFGLARKMN SRADYTNRVI TLWYRPPELL LGTTNYGTEV 180

DMWGCGCLLV ELFNKTAIFQ GSNELEQIES IFKIMGTPTI NSWPTLYDMP WFFMIMPQQT 240

TKYVNNFSEK FKSVLPSSKC LQLAINLLCY DQTKRFSATE ALQSDYF 287

Keyword

ATP-binding; Complete proteome; Cytoplasm; DNA damage; Kinase; mRNA processing; Nucleotide-binding; Nucleus; Phosphoprotein; Protein biosynthesis; Reference proteome; Serine/threonine-protein kinase; Stress response; Transcription; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona intestinalis"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Dipodomys ordii"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Erinaceus europaeus"; ?>Felis catus"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Pongo abelii"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Sus scrofa"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Vicugna pacos"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0032806	; C:carboxy-terminal domain protein kinase complex
GO:0005737	; C:cytoplasm
GO:0005730	; C:nucleolus
GO:0005654	; C:nucleoplasm
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0008353	; F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0031124	; P:mRNA 3'-end processing
GO:0018105	; P:peptidyl-serine phosphorylation
GO:0070816	; P:phosphorylation of RNA polymerase II C-terminal domain
GO:0032786	; P:positive regulation of DNA-dependent transcription, elongation
GO:0045943	; P:positive regulation of transcription from RNA polymerase I promoter
GO:0045903	; P:positive regulation of translational fidelity
GO:0006974	; P:response to DNA damage stimulus
GO:0006351	; P:transcription, DNA-dependent
GO:0006412	; P:translation

KEGG

sce:YKL139W

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR017441	; Protein_kinase_ATP_BS.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; 1.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013