| Tag | Content |
|---|
| EKPD ID | EKS-SAC-00099 |
| Classification | | Group/Family | Score | E-Value | Start | End | Domain Length |
|---|
| CMGC/MAPK | 399.5 | 2.5E-120 | 23 | 301 | 279 |
|
| Status | Reviewed |
| Ensembl Protein | YLR113W |
| UniProt Accession | P32485; D6VYB1; Q06232; Q12294; |
| Protein Name | Mitogen-activated protein kinase HOG1 |
| Protein Synonyms/Alias | MAP kinase HOG1; High osmolarity glycerol response protein 1; |
| Gene Name | HOG1 |
| Gene Synonyms/Alias | HOG1; SSK3; YLR113W; L2931, L9354.2; |
| Ensembl Information | |
| Organism | Saccharomyces cerevisiae |
| Functional Description | Mitogen-activated protein kinase involved in a signaltransduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription via the stress response element (STRE) in promoters of target genes. Upon osmotic shock, associates with the SKO1-SSN6-TUP1 complex, phosphorylates SKO1, and converts it into an activator that subsequently recruits Swi/Snf and SAGA complexes. Activates the SMP1 transcription factor and the RCK2 kinase, both also involved in the regulation of the expression of a subset of osmotic stress-related genes. Phosphorylation of HSL1 by HOG1 leads to a G2 arrest essential for cell survival at high osmolarity. Mediates also cell-cycle arrest in G1 phase by the dual targeting of SIC1. Regulates MFA2 ARE-mediated translation in response to carbon source. Targets RDP3 histone deacetylase to osmoresponsive promoters to induce gene expression on stress. Plays an essential role in maintaining water homeostasis, arsenite detoxification, copper-resistance, hydrogen peroxite response, adaptation to citric acid stress, and repression of the mating pathway activity. Required for the Golgi apparatus localization of MNN1. |
| Protein Length | 435 |
Protein Sequence
(FASTA) | | MTTNEEFIRT QIFGTVFEIT NRYNDLNPVG MGAFGLVCSA TDTLTSQPVA IKKIMKPFST 60 | | AVLAKRTYRE LKLLKHLRHE NLICLQDIFL SPLEDIYFVT ELQGTDLHRL LQTRPLEKQF 120 | | VQYFLYQILR GLKYVHSAGV IHRDLKPSNI LINENCDLKI CDFGLARIQD PQMTGYVSTR 180 | | YYRAPEIMLT WQKYDVEVDI WSAGCIFAEM IEGKPLFPGK DHVHQFSIIT DLLGSPPKDV 240 | | INTICSENTL KFVTSLPHRD PIPFSERFKT VEPDAVDLLE KMLVFDPKKR ITAADALAHP 300 | | YSAPYHDPTD EPVADAKFDW HFNDADLPVD TWRVMMYSEI LDFHKIGGSD GQIDISATFD 360 | | DQVAAATAAA AQAQAQAQAQ VQLNMAAHSH NGAGTTGNDH SDIAGGNKVS DHVAANDTIT 420 | | DYGNQAIQYA NEFQQ 435 |
|
Nucleotide Sequence
(FASTA) | | ATGACCACTA ACGAGGAATT CATTAGGACA CAGATATTCG GTACAGTTTT CGAGATCACA 60 | | AATAGATACA ATGATTTAAA CCCCGTTGGG ATGGGGGCAT TTGGGTTGGT TTGCTCAGCC 120 | | ACGGACACTT TGACATCTCA GCCAGTTGCC ATTAAGAAAA TCATGAAACC TTTTTCCACT 180 | | GCAGTGCTGG CCAAAAGGAC ATATCGTGAA CTAAAACTAC TAAAACATCT AAGACACGAG 240 | | AACTTGATTT GCCTTCAGGA CATATTTCTT TCTCCATTGG AAGATATATA TTTTGTCACG 300 | | GAATTACAAG GAACAGATTT ACATAGACTC TTGCAAACAA GACCCTTGGA AAAGCAATTT 360 | | GTTCAGTATT TCCTATACCA AATTCTAAGG GGTTTAAAAT ACGTTCACTC CGCGGGCGTC 420 | | ATTCATAGAG ATTTGAAACC GAGCAACATT CTGATTAATG AAAACTGTGA TTTGAAGATT 480 | | TGCGATTTCG GTCTAGCAAG AATTCAAGAC CCTCAAATGA CAGGCTATGT TTCCACTAGA 540 | | TACTACAGGG CACCTGAAAT CATGCTAACG TGGCAAAAAT ATGACGTCGA GGTCGACATT 600 | | TGGTCCGCTG GTTGTATTTT TGCCGAAATG ATTGAAGGTA AGCCTTTGTT CCCTGGGAAA 660 | | GATCATGTTC ACCAATTTTC GATCATCACT GACTTGTTGG GATCTCCGCC AAAGGATGTG 720 | | ATAAATACTA TTTGTTCCGA AAATACTCTA AAATTTGTTA CTTCGTTACC ACACAGAGAT 780 | | CCAATTCCAT TTTCTGAAAG ATTTAAAACA GTCGAACCTG ATGCCGTAGA CCTTTTGGAA 840 | | AAAATGCTGG TTTTTGATCC TAAGAAGAGA ATCACTGCGG CGGATGCCTT GGCTCATCCT 900 | | TATTCGGCTC CTTACCACGA TCCAACGGAT GAACCAGTAG CCGATGCCAA GTTCGATTGG 960 | | CACTTTAATG ACGCTGATCT GCCTGTCGAT ACCTGGCGTG TTATGATGTA CTCAGAAATC 1020 | | CTAGACTTCC ATAAGATTGG TGGCAGTGAT GGACAGATTG ATATATCTGC CACGTTTGAT 1080 | | GACCAAGTTG CTGCAGCCAC CGCTGCCGCG GCGCAGGCAC AGGCTCAGGC TCAGGCTCAA 1140 | | GTTCAGTTAA ACATGGCTGC GCATTCGCAT AATGGCGCTG GCACTACTGG AAATGATCAC 1200 | | TCAGATATAG CTGGTGGAAA CAAAGTCAGC GATCATGTAG CTGCAAATGA CACCATTACG 1260 | | GACTACGGTA ACCAGGCCAT ACAGTACGCT AATGAGTTCC AACAGTAA 1308 |
|
| Domain Profile | S: 1 yeslkplgeGaygvvvsavdkrteervaikklsrpfqketsakrtlRElkllkelkheNi 60 | y++l+p+g Ga+g+v+sa+d+ t++ vaikk+++pf++++ akrt+RElkllk+l+heN+ | Q: 23 YNDLNPVGMGAFGLVCSATDTLTSQPVAIKKIMKPFSTAVLAKRTYRELKLLKHLRHENL 82 | 8999******************************************************** |
| S: 61 iklldvftpeeeleelkdvYlvtelmetdLkkviksqklsdehiklllyqilrglkylHs 120 | i+l d+f + l+d+Y+vtel +tdL+++++++ l+++ ++++lyqilrglky+Hs | Q: 83 ICLQDIFLSP-----LEDIYFVTELQGTDLHRLLQTRPLEKQFVQYFLYQILRGLKYVHS 137 | *******655.....59******************************************* |
| S: 121 anviHrDlKPsNllvnedcelkildFGlarsadkekekklteyvatrwYraPeillslke 180 | a+viHrDlKPsN+l+ne+c+lki+dFGlar++d++ +t+yv+tr+YraPei+l++++ | Q: 138 AGVIHRDLKPSNILINENCDLKICDFGLARIQDPQ----MTGYVSTRYYRAPEIMLTWQK 193 | ***********************************....********************* |
| S: 181 ytkavDiWsvGCIlaElltgkplfpgkdeidqlekilevlgtpseeflkkieseearnyi 240 | y+ +vDiWs+GCI+aE+++gkplfpgkd+++q++ i+++lg+p ++++++i+se++ +++ | Q: 194 YDVEVDIWSAGCIFAEMIEGKPLFPGKDHVHQFSIITDLLGSPPKDVINTICSENTLKFV 253 | ************************************************************ |
| S: 241 kslpkkkkkdfeelfpkaseealdLleklLvldpdkRisveeaLehpY 288 | +slp+++ +f+e f++ +++a+dLlek+Lv+dp+kRi++++aL+hpY | Q: 254 TSLPHRDPIPFSERFKTVEPDAVDLLEKMLVFDPKKRITAADALAHPY 301 | ************************************************ |
|
Domain Sequence
(FASTA) | | YNDLNPVGMG AFGLVCSATD TLTSQPVAIK KIMKPFSTAV LAKRTYRELK LLKHLRHENL 60 | | ICLQDIFLSP LEDIYFVTEL QGTDLHRLLQ TRPLEKQFVQ YFLYQILRGL KYVHSAGVIH 120 | | RDLKPSNILI NENCDLKICD FGLARIQDPQ MTGYVSTRYY RAPEIMLTWQ KYDVEVDIWS 180 | | AGCIFAEMIE GKPLFPGKDH VHQFSIITDL LGSPPKDVIN TICSENTLKF VTSLPHRDPI 240 | | PFSERFKTVE PDAVDLLEKM LVFDPKKRIT AADALAHPY 279 |
|
| Keyword | Activator; ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase. |
| Sequence Source | Ensembl |
| Orthology | |
| Gene Ontology | |
| KEGG | |
| InterPros | |
| Pfam | |
| SMARTs | |
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| Prints | |
| Created Date | 20-Feb-2013 |