EKS-SAC-00069

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EKS-SAC-00069

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
CMGC/CDK	298.8	1.2E-89	178	463	286

Status

Reviewed

Ensembl Protein

YPL042C

UniProt Accession

P39073; D6W3X1;

Protein Name

Meiotic mRNA stability protein kinase SSN3

Protein Synonyms/Alias

Cyclin-dependent kinase 8; Suppressor of RNA polymerase B SRB10;

Gene Name

SSN3

Gene Synonyms/Alias

SSN3; ARE1, CDK8, GIG2, SRB10, UME5; YPL042C;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YPL042C	YPL042C	YPL042C

Organism

Saccharomyces cerevisiae

Functional Description

Component of the SRB8-11 complex. The SRB8-11 complex isa regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II- dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II RPB1 at serines 2 and 5. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin pair may also positively and negatively regulate numerous transcriptional activators in response to changes in nutritional and physiological conditions. Phosphorylates GCN4, promoting its ubiquitin-mediated degradation, and MSN2, promoting its nuclear exclusion. Phosphorylates STE12, thereby promoting its degradation and inhibition of filamentous growth. Phosphorylates GAL4, and this phosphorylation is required for efficient galactose-inducible transcription. Also phosphorylates BDF1 and the TAF2 subunit of the TFIID complex.

Protein Length

555

Protein Sequence
(FASTA)

MYNGKDRAQN SYQPMYQRPM QVQGQQQAQS FVGKKNTIGS VHGKAPMLMA NNDVFTIGPY 60

RARKDRMRVS VLEKYEVIGY IAAGTYGKVY KAKRQINSGT NSANGSSLNG TNAKIPQFDS 120

TQPKSSSSMD MQANTNALRR NLLKDEGVTP GRIRTTREDV SPHYNSQKQT LIKKPLTVFY 180

AIKKFKTEKD GVEQLHYTGI SQSACREMAL CRELHNKHLT TLVEIFLERK CVHMVYEYAE 240

HDLLQIIHFH SHPEKRMIPP RMVRSIMWQL LDGVSYLHQN WVLHRDLKPA NIMVTIDGCV 300

KIGDLGLARK FHNMLQTLYT GDKVVVTIWY RAPELLLGAR HYTPAVDLWS VGCIFAELIG 360

LQPIFKGEEA KLDSKKTVPF QVNQLQRILE VLGTPDQKIW PYLEKYPEYD QITKFPKYRD 420

NLATWYHSAG GRDKHALSLL YHLLNYDPIK RIDAFNALEH KYFTESDIPV SENVFEGLTY 480

KYPARRIHTN DNDIMNLGSR TKNNTQASGI TAGAAANALG GLGVNRRILA AAAAAAAAVS 540

GNNASDEPSR KKNRR 555

Nucleotide Sequence
(FASTA)

ATGTATAATG GCAAGGATAG AGCACAAAAC TCCTATCAGC CAATGTACCA AAGGCCTATG 60

CAGGTACAAG GACAACAGCA AGCTCAATCG TTCGTTGGAA AGAAAAACAC AATCGGAAGT 120

GTGCATGGAA AAGCCCCGAT GCTAATGGCC AATAATGATG TTTTTACTAT TGGACCTTAT 180

AGGGCAAGAA AAGATAGAAT GCGGGTATCT GTCTTAGAAA AGTACGAAGT TATTGGCTAC 240

ATTGCTGCGG GCACATATGG TAAAGTTTAC AAAGCGAAAA GACAAATCAA CTCCGGTACC 300

AATTCCGCTA ATGGTTCTAG TCTGAATGGT ACCAATGCGA AAATTCCGCA GTTTGACAGC 360

ACGCAACCAA AATCAAGCTC TTCAATGGAC ATGCAGGCAA ATACAAACGC ATTAAGAAGA 420

AACTTGTTAA AGGATGAAGG AGTGACCCCC GGAAGAATAC GAACTACGAG GGAAGATGTA 480

TCCCCGCACT ATAATTCCCA AAAACAAACC CTCATTAAAA AACCGCTGAC GGTATTTTAT 540

GCCATTAAAA AGTTCAAGAC AGAGAAGGAT GGCGTCGAAC AATTGCATTA TACGGGAATA 600

TCTCAGAGTG CCTGTAGAGA AATGGCATTA TGTCGAGAAT TGCACAACAA GCATTTAACC 660

ACATTAGTGG AAATTTTTTT GGAAAGGAAA TGTGTCCATA TGGTATACGA ATATGCGGAG 720

CATGATCTGC TACAAATTAT CCACTTCCAT TCCCATCCCG AAAAAAGGAT GATACCACCA 780

AGAATGGTTC GGTCTATTAT GTGGCAGCTT TTAGACGGCG TATCGTATCT TCATCAAAAT 840

TGGGTGCTTC ATCGAGATTT GAAACCCGCA AATATAATGG TGACCATAGA TGGATGTGTT 900

AAAATTGGTG ATTTAGGTTT GGCCAGAAAA TTTCATAATA TGCTGCAAAC CCTCTATACT 960

GGGGATAAAG TGGTTGTCAC TATATGGTAC CGTGCACCTG AGTTGCTATT GGGAGCACGG 1020

CACTATACCC CTGCGGTTGA TTTATGGTCC GTTGGCTGCA TTTTTGCAGA ACTGATAGGA 1080

TTACAGCCCA TATTTAAAGG TGAAGAAGCT AAACTAGACT CTAAAAAGAC TGTTCCATTT 1140

CAAGTGAATC AACTACAGAG AATTTTGGAA GTTCTTGGCA CTCCCGATCA AAAAATTTGG 1200

CCTTATTTGG AGAAGTATCC AGAATATGAT CAAATTACGA AGTTTCCAAA GTATAGGGAT 1260

AACCTTGCTA CATGGTATCA TTCCGCGGGA GGAAGGGACA AGCATGCTTT AAGCTTACTT 1320

TACCACTTGT TAAATTATGA TCCAATTAAA AGAATAGATG CATTTAATGC GTTGGAACAT 1380

AAGTACTTCA CAGAAAGTGA TATTCCTGTT AGTGAAAATG TATTTGAAGG TCTAACTTAC 1440

AAATACCCGG CAAGAAGAAT TCACACGAAC GATAATGACA TCATGAATCT TGGATCAAGA 1500

ACGAAAAACA ATACACAAGC TTCAGGAATC ACCGCAGGTG CCGCTGCAAA TGCGTTAGGT 1560

GGGCTTGGTG TTAACCGTAG AATTCTGGCC GCGGCAGCAG CAGCCGCTGC TGCGGTGTCA 1620

GGAAACAATG CATCAGATGA GCCATCTCGA AAGAAAAACA GAAGATAG 1668

Domain Profile

S: 25    evvAlKkirlekek......egvpitalrEisllkelkhknivkLlevvakdkkklyLvf  78

          ++A+Kk+++ek+       +g++++a+rE++l++el++k++++L+e++  ++k +++v+

Q: 178   VFYAIKKFKTEKDGveqlhyTGISQSACREMALCRELHNKHLTTLVEIFL-ERKCVHMVY  236

         469********9987667667*****************************.99*******

S: 79    efleqdLkkllkkkkkk...klsleevkslllqlleglaylHsnkilHRDlKpqNlLisk  135

         e++e+dL ++++ +++    ++++++v+s+++qll+g++ylH+n++lHRDlKp+N++++

Q: 237   EYAEHDLLQIIHFHSHPekrMIPPRMVRSIMWQLLDGVSYLHQNWVLHRDLKPANIMVTI  296

         ***********9998877779***************************************

S: 136   egklklaDfGlarafssplkt.yt..kevvTlwYraPelLlgakeYstavDiWsvgcila  192

         +g++k++D+Glar+f+++l+t yt  k+vvT+wYraPelLlga++Y+ avD+Wsvgci+a

Q: 297   DGCVKIGDLGLARKFHNMLQTlYTgdKVVVTIWYRAPELLLGARHYTPAVDLWSVGCIFA  356

         ******************9975665589********************************

S: 193   elltrkplfqgkse...........idqlerifkllgtpsekvwpevsklpeyk..ksfp  239

         el+  +p+f+g++            ++ql+ri+++lgtp++k+wp ++k+pey+  ++fp

Q: 357   ELIGLQPIFKGEEAkldskktvpfqVNQLQRILEVLGTPDQKIWPYLEKYPEYDqiTKFP  416

         ************999***************************************644666

S: 240   kkkkkklkekvkklk...ekaldllekllaldpkkRisakealqhkyf  284

         k+ + +l+++++++    ++al+ll +ll++dp kRi+a +al+hkyf

Q: 417   KY-RDNLATWYHSAGgrdKHALSLLYHLLNYDPIKRIDAFNALEHKYF  463

         63.33777777776554367***************************9

Domain Sequence
(FASTA)

VFYAIKKFKT EKDGVEQLHY TGISQSACRE MALCRELHNK HLTTLVEIFL ERKCVHMVYE 60

YAEHDLLQII HFHSHPEKRM IPPRMVRSIM WQLLDGVSYL HQNWVLHRDL KPANIMVTID 120

GCVKIGDLGL ARKFHNMLQT LYTGDKVVVT IWYRAPELLL GARHYTPAVD LWSVGCIFAE 180

LIGLQPIFKG EEAKLDSKKT VPFQVNQLQR ILEVLGTPDQ KIWPYLEKYP EYDQITKFPK 240

YRDNLATWYH SAGGRDKHAL SLLYHLLNYD PIKRIDAFNA LEHKYF 286

Keyword

Activator; ATP-binding; Complete proteome; Kinase; Magnesium; Meiosis; Metal-binding; Nucleotide-binding; Nucleus; Reference proteome; Repressor; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Ciona savignyi"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Equus caballus"; ?>Felis catus"; ?>Gadus morhua"; ?>Gallus gallus"; ?>Gasterosteus aculeatus"; ?>Gorilla gorilla"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Ochotona princeps"; ?>Oreochromis niloticus"; ?>Ornithorhynchus anatinus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Pongo abelii"; ?>Procavia capensis"; ?>Pteropus vampyrus"; ?>Rattus norvegicus"; ?>Sarcophilus harrisii"; ?>Taeniopygia guttata"; ?>Takifugu rubripes"; ?>Tarsius syrichta"; ?>Tetraodon nigroviridis"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0016592	; C:mediator complex
GO:0005524	; F:ATP binding
GO:0004693	; F:cyclin-dependent protein kinase activity
GO:0046872	; F:metal ion binding
GO:0008353	; F:RNA polymerase II carboxy-terminal domain kinase activity
GO:0007126	; P:meiosis
GO:0060258	; P:negative regulation of filamentous growth
GO:0000122	; P:negative regulation of transcription from RNA polymerase II promoter
GO:0070481	; P:nuclear-transcribed mRNA catabolic process, non-stop decay
GO:0070816	; P:phosphorylation of RNA polymerase II C-terminal domain
GO:0000435	; P:positive regulation of transcription from RNA polymerase II promoter by galactose
GO:0031648	; P:protein destabilization
GO:0006351	; P:transcription, DNA-dependent

KEGG

sce:YPL042C

;

InterPros

IPR011009	; Kinase-like_dom.
IPR000719	; Prot_kinase_cat_dom.
IPR002290	; Ser/Thr_dual-sp_kinase_dom.
IPR008271	; Ser/Thr_kinase_AS.

Pfam

PF00069

; Pkinase; 1.

SMARTs

SM00220

; S_TKc; 1.

Prosites

PS00107	; PROTEIN_KINASE_ATP; FALSE_NEG.
PS50011	; PROTEIN_KINASE_DOM; 1.
PS00108	; PROTEIN_KINASE_ST; 1.

Prints

Created Date

20-Feb-2013