EPS-SAC-00024

Eukaryotic Protein Kinase & Protein Phosphatase Database

Tag

Content

EKPD ID

EPS-SAC-00024

Classification

Group/Family	Score	E-Value	Start	End	Domain Length
Asp-Based PTP/FCP	148.1	7.1E-44	192	339	148

Status

Reviewed

Ensembl Protein

YPL063W

UniProt Accession

Q02776; D6W3V2;

Protein Name

Mitochondrial import inner membrane translocase subunit TIM50

Protein Synonyms/Alias

Gene Name

TIM50

Gene Synonyms/Alias

TIM50; YPL063W;

Ensembl Information

Ensembl Gene ID	Ensembl Protein ID	Ensembl Transcript ID
YPL063W	YPL063W	YPL063W

Organism

Saccharomyces cerevisiae

Functional Description

Essential component of the TIM23 complex, a complex thatmediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex. Does not act as a structural subunit of the TIM23 complex but plays a dynamic role in protein import. Has no phosphatase activity.

Protein Length

476

Protein Sequence
(FASTA)

MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD KPKSILTDDM 60

LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK RQTSTDIKRE KYANWFYIFS 120

LSALTGTAIY MARDWEPQES EELKKDIDNG YTLSLMYKRF KARFNSMFTY FQEPPFPDLL 180

PPPPPPPYQR PLTLVITLED FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN 240

YMMYSDKIAE KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK 300

LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN LAEEFDHRVK 360

KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE EGQKNYLMFM KMIEEEKEKI 420

RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK IQLEKQKEVD ALFEEEKKKK KIAESK 476

Nucleotide Sequence
(FASTA)

ATGCTGTCCA TTTTAAGAAA TTCGGTGAGA CTAAATTCAA GGGCTCTTAG GGTTGTGCCA 60

TCTGCCGCCA ACACCTTAAC ATCGGTTCAA GCATCCAGAA GACTTTTAAC AAGTTATTCA 120

AGTTTTTTAC AAAAAGAAAC AAAAGACGAC AAGCCTAAAT CCATTCTTAC AGATGATATG 180

CTGTTCAAGG CCGGTGTTGA CGTCGATGAG AAGGGCCAAG GCAAAAACGA GGAAACATCA 240

GGAGAAGGAG GAGAGGACAA GAATGAACCT TCTTCCAAAA GTGAAAAATC TAGAAGAAAA 300

AGACAAACTT CTACAGATAT TAAAAGAGAA AAGTATGCTA ACTGGTTTTA CATTTTTTCG 360

TTGTCTGCGT TGACAGGTAC TGCAATCTAC ATGGCAAGGG ATTGGGAGCC TCAAGAGTCT 420

GAAGAATTGA AGAAAGACAT CGATAATGGC TACACTTTAT CACTTATGTA TAAAAGATTC 480

AAGGCCAGGT TCAACTCAAT GTTCACCTAC TTCCAAGAGC CACCTTTCCC TGATTTACTA 540

CCTCCACCAC CACCACCACC GTACCAAAGG CCATTAACTC TTGTTATCAC ATTGGAAGAT 600

TTTTTGGTTC ATTCTGAGTG GTCTCAAAAG CATGGTTGGA GAACGGCCAA AAGACCTGGT 660

GCTGACTACT TCTTGGGTTA CCTATCGCAG TATTACGAAA TTGTTTTGTT TTCATCCAAC 720

TATATGATGT ACTCTGACAA AATCGCTGAA AAATTAGATC CAATCCATGC ATTCGTATCT 780

TATAATTTGT TCAAGGAACA CTGTGTTTAC AAAGACGGTG TGCACATTAA GGATCTGTCA 840

AAATTGAATA GAGATTTGAG TAAAGTAATC ATTATTGACA CTGACCCTAA CAGTTACAAA 900

TTGCAACCTG AAAATGCTAT TCCAATGGAG CCATGGAATG GTGAAGCTGA TGACAAATTA 960

GTAAGATTGA TTCCATTTTT GGAGTACCTT GCCACTCAAC AAACCAAGGA TGTTAGACCA 1020

ATCTTGAACA GCTTTGAAGA CAAGAAGAAC CTAGCAGAAG AATTTGATCA TCGTGTGAAA 1080

AAATTGAAGG ATAAATTTTA CGGAGATCAT AAATCTGGTG GCAACTGGGC AATGACGGCA 1140

CTAGGTCTCG GAAATTCCCT GGGCGGCAGC ACCAAGTTCC CGCTCGATTT GATTCATGAA 1200

GAAGGACAAA AGAACTATTT AATGTTCATG AAGATGATTG AGGAAGAAAA GGAAAAAATT 1260

AGAATACAGC AGGAGCAAAT GGGCGGGCAA ACATTTACGC TGAAAGACTA TGTTGAAGGT 1320

AACTTGCCTT CGCCAGAAGA ACAAATGAAA ATACAATTGG AGAAGCAGAA GGAGGTAGAC 1380

GCCTTATTTG AAGAGGAAAA GAAAAAGAAG AAGATTGCTG AATCCAAATA A 1431

Domain Profile

S: 1     ktLVLDLDetLvhsksekdeeeektkasvkkRPgldeFLkelskayevviftasskeyad  60

         +tLV+ L++ Lvhs+++++     + ++++kRPg+d+FL +ls++ye+v+f++  ++y d

Q: 192   LTLVITLEDFLVHSEWSQK-----HGWRTAKRPGADYFLGYLSQYYEIVLFSSNYMMYSD  246

         69*************9776.....9***********************************

S: 61    avldkldpkkklfkkrlyresctlengkyvKdLsllgrdlskvvivDdsprsfelqpdnl  120

         ++ +kldp + +++++l++e+c++++g ++KdLs+l+rdlskv+i+D +p+s++lqp+n+

Q: 247   KIAEKLDPIHAFVSYNLFKEHCVYKDGVHIKDLSKLNRDLSKVIIIDTDPNSYKLQPENA  306

         ********99999**************99*******************************

S: 121   ipiepfygdsdkdtellkllpfleale.ks.ddvr  153

         ip+ep++g+   d++l +l+pfle+l+ ++ +dvr

Q: 307   IPMEPWNGE--ADDKLVRLIPFLEYLAtQQtKDVR  339

         ******998..5569************96669997

Domain Sequence
(FASTA)

LTLVITLEDF LVHSEWSQKH GWRTAKRPGA DYFLGYLSQY YEIVLFSSNY MMYSDKIAEK 60

LDPIHAFVSY NLFKEHCVYK DGVHIKDLSK LNRDLSKVII IDTDPNSYKL QPENAIPMEP 120

WNGEADDKLV RLIPFLEYLA TQQTKDVR 148

Keyword

Complete proteome; Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport; Reference proteome; Transit peptide; Translocation; Transmembrane; Transmembrane helix; Transport.

Sequence Source

Ensembl

Orthology

Ortholog group

Ailuropoda melanoleuca"; ?>Anolis carolinensis"; ?>Bos taurus"; ?>Caenorhabditis elegans"; ?>Callithrix jacchus"; ?>Canis familiaris"; ?>Cavia porcellus"; ?>Danio rerio"; ?>Drosophila melanogaster"; ?>Echinops telfairi"; ?>Equus caballus"; ?>Felis catus"; ?>Gasterosteus aculeatus"; ?>Homo sapiens"; ?>Ictidomys tridecemlineatus"; ?>Latimeria chalumnae"; ?>Loxodonta africana"; ?>Macaca mulatta"; ?>Macropus eugenii"; ?>Meleagris gallopavo"; ?>Monodelphis domestica"; ?>Mus musculus"; ?>Mustela putorius furo"; ?>Myotis lucifugus"; ?>Nomascus leucogenys"; ?>Oreochromis niloticus"; ?>Oryctolagus cuniculus"; ?>Oryzias latipes"; ?>Otolemur garnettii"; ?>Pan troglodytes"; ?>Pelodiscus sinensis"; ?>Petromyzon marinus"; ?>Rattus norvegicus"; ?>Sus scrofa"; ?>Tetraodon nigroviridis"; ?>Tursiops truncatus"; ?>Xenopus tropicalis"; ?>Xiphophorus maculatus"; ?>Schizosaccharomyces pombe"; ?>

Gene Ontology

GO:0016021	; C:integral to membrane
GO:0005744	; C:mitochondrial inner membrane presequence translocase complex
GO:0030943	; F:mitochondrion targeting sequence binding
GO:0030150	; P:protein import into mitochondrial matrix

KEGG

sce:YPL063W

;

InterPros

IPR023214	; HAD-like_dom.
IPR004274	; NIF.

Pfam

PF03031

; NIF; 1.

SMARTs

SM00577

; CPDc; 1.

Prosites

PS50969

; FCP1; 1.

Prints

Created Date

20-Feb-2013