※ H11 family introduction

    H11 (HSPB8, HSP22) belongs to the family of "small heat-shock protein" and is classified into atypical protein kinase group (1). The kinase activity was first observed when H11-GST fusion protein was purified from bacteria and autophosphorylated in an in vitro kinase assay. In addition, immunoprecipitation with an anti-H11 antibody in human 293 cells revealed a phosphoprotein, which can't be detected when a predicted catalytic lysine was mutated. However, later studies show that the lysine residue may not responsible for the catalytic reaction and the kinase activity is due to a partner protein which binds H11 (2).

Reference
1. Manning, G., Whyte, D.B., Martinez, R., Hunter, T. and Sudarsanam, S. (2002) The protein kinase complement of the human genome. Science, 298, 1912-1934. PMID: 12471243
2. Wikinome annotation: Kinase Family H11